Cytotoxic Effect of Ethanolic Extract Fractions of Indonesia Plant Ficus septica Burm. F. on Human Breast Cancer T47D cell lines

Authors

  • Agung Endro Nugroho Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta, Indonesia.
  • Muthi Ikawati Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta, Indonesia. 55281
  • Adam Hermawan Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta, Indonesia. 55281
  • Dyaningtyas Dewi Pamungkas Putri Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta, Indonesia. 55281
  • Edy Meiyanto Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta, Indonesia. 55281

Keywords:

Ficus septica Burm. F., cancer, T47D cells, cytotoxic

Abstract

Ficus septica Burm. F. (Moraceae) is a plant which grows widely in some areas of Indonesia and other Southeast Asia countries. Previously, ethanolic extract of the plant leaves exhibit cytotoxic effect on several cell lines including T47D cells. The extract also showed chemoprevention effect in vivo. In the study, the ethanolic extract was fractionated gradually by n-hexane and ethyl acetate to yield four fractions including n-hexane soluble fraction, n-hexane insoluble fraction, ethyl acetate soluble fraction and ethyl acetate insoluble fraction. These fractions were then investigated for their cytotoxic effect on T47D cells. The cell viability were assessed using MTT colorimetric assay. The results showed that the cytotoxic effect of nhexane insoluble fraction (IC50 : 9.3 µg/mL) was much more potent than this of n-hexane soluble fraction (IC50 : 331.0 µg/mL). Hexane insoluble fraction was then fractionated with ethyl acetate to provide ethyl acetate soluble fraction and ethyl acetate insoluble fraction. The cytotoxic effect of ethyl acetate soluble fraction (IC50 : 13.7 µg/mL) was much more potent than this of ethyl acetate insoluble fraction (IC50: >700 µg/mL). In conclusion, n-hexane insoluble fraction and ethyl acetate soluble fraction exhibited potent cytotoxic on breasr cancer T47D cell lines. The fractions are potential to be developed as anticancer agent in breast cancer therapy.

References

Lullmann H, Mohr K, Ziegler A, Bieger

D. Color Atlas of Pharmacology. 2nd Ed.

Georg Thieme Verlag, Stuttgart,

Germany. 2000;pp. 296-299.

Rang HP, Dale MM, Ritter JM, Moore

PK. Pharmacology. 5th Ed. Churchill

Livingstone, Sydney. 2003;pp.693-698.

WHO 2011, Cancer, World Health

Organization, Switzerland. 2011.

http://www.who.int/mediacentre/factsheet

s/fs297/en/

Jemal A, Siegel R, Xu J, Ward E. Cancer

statistics, Ca Cancer J Clin. 2010;60:277–

Tjindarbumi D, Mangunkusumo R,

Cancer in Indonesia, Present and Future,

Jpn J Clin Oncol. 2002;32 (Supplement

:S17-S21.

Pratama RH, Ikhtiarsyah YG, Fitriasari A,

Anindyajati, Ikawati M, Meiyanto E.

Awar-awar Leaves Ehanolic Extract

Sinergistically Enhances Cytotoxic of

Doxorubicin on T47D Breast Cancer

Cells, Indo J Pharm Sci. 2011;9(1):67-71.

Sekti DA, Mubarok MF, Armandani I,

Junedy S, Meiyanto E. Awar–Awar (Ficus

Septica Burm. F.) Leaves Ethanolic

Extract Induced Apoptosis of MCF-7

Cells By Downregulation of Bcl-2, J Trad

Med 2010;15(3).

Septhea DB, Anindyajati, Darma A,

Nurzijah I, Nugroho AE. Ficus septica

burm. F. Leaves Ethanolic Extract

Induces Apoptosis in 7,12-

dimethylbenz[a]nthracene-induced Rat

Liver Cancer Quantitatively, Indo J

Cancer Chemoprev. 2010;2(2):242-248.

Kirana C, Record IR, McIntosh GH, Jones

GP. Screening for Antitumor Activity of

Species of Indonesian Zingiberaceae

Using Human MCF-7 and HT-29 Cancer

Cells, Pharmaceut Biol. 2003;41(4):271-

Sugiyanto, Sudarto B, Meiyanto E,

Nugroho AE. The Anticarcinogenic

Activity of Plants Compounds, Indo J

Pharm. 2003;14(3):132-141.

Kumala S, Septiseptyani EP, Meiyanto E.

Cytotoxic effect of secondary metabolites

produced by endopytic fungi 1.3.11, 1.1.6

and 1.2.6 isolated from the fruit of

“Tanaman Buah Makassar” (Brucea

janvanica (l.) Merr) on in-vitro T47D and

MCF-7 intact cells and identification of

the fungus 1.3.11 by ribosomal DNA

sequence analysis, Int J Pharm

Pharmaceut Sci. 2010;2(2):80-83.

Nurrochmad A, Lukitaningsih E,

Meiyanto E. Anti cancer activity of rodent

tuber (Thyphonium flagelliforme (lodd.)

Blume) on human breast cancer T47D

cells, Int J Phytomedicine. 2011;3:138-

Meiyanto E, Handayani S, Susidarti RA,

Jenie RI. Chloroform fraction of Areca

(Areca catechu L.,) Induces apoptosis an

decreases Bcl-2 expression on MCF-7

cells, Mal J Pharm 2009;1(7):290-294.

Meiyanto E, Handayani S, Jenie RI.

Snake beans (Vigna sinensis (L) Savi ex

Hassk) extract increases breast epithelial

cells proliferation, Indo J Pharm.

;19(4):191-197.

Sarmoko Ratri ID, Febriansah R,

Romadhon AF, Nugroho APA, Meiyanto

E, Susidarti RA and Sudarmanto BA.

Cytotoxic effect of ethanolic extract of

Temu Kunci (Kaempferia pandurata) and

Sirihan (Piper aduncum L.) on breast

cancer line, Proceeding The International

symposium on Molecular targeted

Therapy. Faculty of Pharmacy UGM.

;pp. 94-102.

Pebriana RB, Wardhani BWK, Widayanti

E, Wijayanti NLS, Wijayanti TR, Riyanto

S, Meiyanto E. Apoptotic Effect of

Kenikir Leaves (Cosmos caudatus Kunth.)

Methanolic Extract on Breast Cancer Cell

Line, Pharmacon. 2008;9(1);21-26.

Wu PL, Rao KV, Su CH, Kuoh CS, Wu

TS. Phenanthroindolizidine Alkaloids and

Their Cytotoxicity from the Leaves of

Ficus septica, Heterocycles.

;57:2401–2408.

Ueda JY, Takagi M, Shinya K.

Aminocaprophenone- and pyrrolidinetype alkaloids from the leaves of Ficus

septica, J Nat Prod. 2009;72(12):2181-

Damu AG, Kuo PC, Shi LS, Li CY, Kuoh

CS, Wu PL, Wu TS.

Phenanthroindolizidine alkaloids from the

stems of Ficus septica, J Nat Prod.

;68(7):1071-1075.

Damu AG, Kuo PC, Shi LS, Li CY, Su

CR, Wu TS. Cytotoxic

phenanthroindolizidine alkaloids from the

roots of Ficus septica, Planta Med.

;75(10):1152-1156.

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Published

30-06-2011

How to Cite

1.
Agung Endro Nugroho, Muthi Ikawati, Adam Hermawan, Dyaningtyas Dewi Pamungkas Putri, Edy Meiyanto. Cytotoxic Effect of Ethanolic Extract Fractions of Indonesia Plant Ficus septica Burm. F. on Human Breast Cancer T47D cell lines. ijp [Internet]. 2011 Jun. 30 [cited 2024 Dec. 3];3(2):216-2. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/99

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Original Research Articles