Ethanolic Extracts Of A. Paniculata (burm. f.) Nees And Its Active Compound, Andrographolide, Decrease The Expression Of Glucose Transporters (glut 4) In High Fuctose-Fat Fed Rats

Authors

  • Agung Endro Nugroho Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281.
  • Mohamad Andrie Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281.
  • Rina Susilowati Department of Histology and Cell Biology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281.
  • Arief Nurrochmad Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281.
  • Endang Lukitaningsih Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281
  • Suwijiyo Pramono Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281.

Keywords:

Andrographis paniculata (Burm. f.) Nees, andrographolide, insulin resistance, GLUT-4

Abstract

Andrographolide is a major compound (> 4%) of Andrographis paniculata (Burm. f.) Nees. The compound is most active than the other compounds contained in the plant. Previously, the compound was reported showing hypoglycemic activity in type 1 diabetes mellitus (DM) rats. The compound also improved the uptake of glucose in isolated soleus muscle of type 1 DM rats by increasing the GLUT4 expression. The disruption of GLUT4 translocation is a major factor of insulin resistance. In the study, we investigated the effect of andrografolid and ethanolic extract of A. paniculata (Burm. f.) Nees on the GLUT4 expression in high fuctose-fat fed rats, a model of insulin resistant rats. Insulin resistance in rats was induced by high fructose-fat containing 36% fructose, 15% lard and 5% egg yolks in 0.36 g/200g BW for 55 days. In the study, andrographolide and ethanolic extract of A. paniculata (Burm. f.) showed potent hypoglycaemic effects in insulin resistant rats. In addition, these treatments and metformin could restore the depleted GLUT-4 expression in insulin resistant rats. In conclusion, andrographolide and ethanolic extract of A. paniculata (Burm. f.) decreased the blood glucose levels by increasing the GLUT-4 expression in insulin resistant rats.

References

Greenspan FS, Strewler GJ. Basic and Clinical

Endocrinology. 5th Ed. Prentice-Hall

International Inc. Sydney. 1997;605-609.

Rang HP, Dale MM, Ritte JM. Pharmacology.

th Ed. Churchill Livingstone. Melbourne.

;385-340.

Tisch R, McDevitt H. Insulin-Dependent

Diabetes Mellitus. Cell, 1996;85:291-297.

Bastaki S. Diabetes Mellitus and Its

Treatment. Int J Diabetes & Metabolism.

;13:111-134

Bailey CJ, Day C. Traditional plant medicines

as treatments for diabetes. Diabetes Care,

;12(8):553-564.

6.Srinivasan K. Plant foods in the

management of diabetes mellitus: spices as

beneficial antidiabetic food adjuncts. Int J

Food Sci Nutr. 2005;56(6):399-414.

Niranjan A, Tewari S, Lehri A. Biological

activities of Kalmegh (Andrograpis paniculata

Nees. Indian J Nat Proc Resour,

;1(2):125-135.

Zhang XF, Tan BK, Anti-diabetic property of

ethanolic extract of Andrographis paniculata in

streptozotocin-diabetic rats. Acta Pharmacol

Sin, 2000;1(12):1157-1164.

Dandu AM, Inamdar NM. Evaluation of

beneficial effects of antioxidant properties of

aqueous leaf extract of Andrographis

paniculata in STZ-induced diabetes. Pak J

Pharm Sci, 2009;22(1):49-52.

Cheung HY, Cheung CS, Kong CK.

Determination of bioactive diterpenoids from

Andrographis paniculata by micellar

electrokinetic chromatography. J Chromatogr

A 2001;930(1-2):171-176.

Pholphana N, Rangkadilok N, Thongnest S,

Ruchirawat S, Ruchirawat M, Satayavivad J.

Determination and variation of three active

diterpenoids in Andrographis paniculata

(Burm.f.) Nees. Phytochem Anal,

;15(6):365-371.

Burgos RA, Caballero EE, Sánchez NS,

Schroeder RA, Wikman GK, Hancke JL.

Testicular toxicity assessment of Andrographis

paniculata dried extract in rats. J

Ethnopharmacol, 1997;58(3):219-224.

Yu BC, Hung CR, Chen WC, Cheng JT.

Antihyperglycemic effect of andrographolide in

streptozotocin-induced diabetic rats. Planta

Med, 2003;69(12):1075-1079.

Subramanian R, Asmawi MZ, Sadikun A. In

vitro alpha-glucosidase and alpha-amylase

enzyme inhibitory effects of Andrographis

paniculata extract and andrographolide. Acta

Biochim Pol, 2008;55(2):391-398.

Suga A, Hirano T, Kageyama H, Osaka T,

Namba Y, Tsuji M, Miura M, Adachi M, Inoue.

Effects of fructose and glucose on plasma

leptin, insulin, and insulin resistance in lean

and VMH-lesioned obese rats. Am J Physiol

Endocrinol Metab, 2000;278:E677-E683.

Pooranaperundevi M, Sumiyabanu MS,

Viswanathan P, Sundarapandiyan R,

Anuradha CV. Insulin resistance induced by a

high- fructose diet potentiates thioacetamide

hepatotoxicity. Singapore Med,

;J51(5):389-398.

Chi TC, Liu IM, Cheng JT. Less of insulin

desensitization in sympathetic nerve terminals

from wistar rats with insulin resistance. J

Auton Nerv Syst, 2000;80(1-2):80-84.

Katja CZ, Mario S, Artur-Aron W, Franz B,

Helmut EG, Karsten S. Cyclooxygenase-2

expression in human esophageal carcinoma.

Cancer Res, 1999;59:98-204.

Akiba T, Yaguchi K, Tsutsumi K, Nishioka T,

Koyama I, Nomura M, Yokogawa K, Moritani

S, Miyamoto K. Inhibitory mechanism of

caffeine on insulin-stimulated glucose uptake

in adipose cells. Biochem Pharmacol, 2004;

(10):1929-1937.

Bessesen DH. The role of carbohydrates in

insulin resistance. J Nutr,

;131(10):2782S-2786S.

Malinauskas BM, Aeby VG, Overton RF,

Carpenter-Aeby T, Barber-Heidal K. A survey

of energy drink consumption patterns among

college students. Nutr J, 2007;6:35.

Patel JV, Cummings DE, Girod JP,

Mascarenhas AV, Hughes EA, Gupta M, Lip

GY, Reddy S, Brotman DJ. Role of

metabolically active hormones in the insulin

resistance associated with short-term

glucocorticoid treatment. J Negat Results

Biomed, 2006;5:4.

Shulman GI. Cellular mechanisms of insulin

resistance. J Clin Invest, 2000;106(2):171-176.

Marette A, Burdett E, Douen A, Vranic M, Klip

A. Insulin induces the translocation of GLUT4

from a unique intracellular organelle to

transverse tubules in rat skeletal muscle.

Diabetes, 1992;41(12):1562-1569.

Watson RT, Pessin JE. Intracellular

organization of insulin signaling and GLUT4

translocation. Recent Prog Horm Res,

;56:175-193.

Mueckler M. Insulin resistance and the

disruption of Glut4 trafficking in skeletal

muscle. J Clin Invest, 2001;107(10):1211-

Fernandez-Veledo S, Nieto-Vazquez I, de

Castro J, Ramos MP, Brüderlein S, Möller P,

Lorenzo M. Hyperinsulinemia induces insulin

resistance on glucose and lipid metabolism in

a human adipocytic cell line: paracrine

interaction with myocytes. J Clin Endocrinol

Metab, 2008;93(7):2866-2876.

D’Angelo G, Elmarakby AA, Pollock DM,

Stepp DW. Fructose Feeding Increases Insulin

Resistance but Not Blood Pressure in

Sprague-Dawley Rats. Hypertension,

;46:806-811.

Elliott SS, Keim NL, Stern JS, Teff K, Havel

PJ. Fructose, weight gain, and the insulin

resistance syndrome. Am J Clin Nutr,

;76:911–922.

Henquin JC. Pathways in b-Cell StimulusSecretion Coupling as Targets for Therapeutic

Insulin Secretagogues. Diabetes, 2004;53

(Suppl. 3):S48–S58.

Klip A, Leiter L. Cellular Mechanism of Action

of Metformin. Diabetes Care, 1990;13(6):696-

Widyawati T. Aspek Farmakologi Sambiloto

(Andrographis paniculata Nees). Majalah

Kedokteran Nusantara, 2007;40(3):216-222.

Rao YK, Vimalamma G, Rao CV, Tzeng YM.

Flavonoids and andrographolides from

Andrographis paniculata. Phytochemistry,

;65:2317–2321.

Chao WW, Lin BF. Isolation and identification

of bioactive compounds in Andrographis

paniculata (Chuanxinlian). Chinese Medicine,

;5:17

Yu BC, Chang CK, Su CF, Cheng JT.

Mediation of beta-endorphin in

andrographolide-induced plasma glucoselowering action in type I diabetes-like animals.

Naunyn Schmiedebergs Arch Pharmacol,

;377(4-6):529-540.

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Published

31-12-2011

How to Cite

1.
Agung Endro Nugroho, Mohamad Andrie, Rina Susilowati, Arief Nurrochmad, Endang Lukitaningsih, Suwijiyo Pramono. Ethanolic Extracts Of A. Paniculata (burm. f.) Nees And Its Active Compound, Andrographolide, Decrease The Expression Of Glucose Transporters (glut 4) In High Fuctose-Fat Fed Rats. ijp [Internet]. 2011 Dec. 31 [cited 2024 Feb. 29];3(4):486-97. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/130

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