Anti-allergic effects of Marmin, a coumarine isolated from Aegle marmelos Correa: In vitro study

Authors

  • Agung Endro Nugroho Dept of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta, Indonesia 55281
  • Sugeng Riyanto Dept of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada. University, Jogjakarta 55281, Indonesia.
  • Mohamad Aspollah Sukari Department ofChemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia.
  • Kazutaka Maeyama Dept of Pharmacology, Informational Biomedicine, Ehime University Graduate School of Medicine, Shitsugawa, Toon-shi, Ehime 791-0295, Japan.

Keywords:

Aegle marmelos Correa, marmin, histamine release, mast cell

Abstract

Marmin or (7-(6’,7’-dihydroxygeranyl-oxy)coumarin is an active compound isolated from Aegle marmelos Correa. The study aimed to investigate the effects of marmin on the histamine release from rat mast cell. The study was performed by using rat basophilic leukemia (RBL- 2H3) cell line and rat peritoneal mast cells (RPMCs). The histamine release from these cells was determined by using HPLC fluorometric method. In this study, marmin succeeded to inhibit the histamine release from RBL-2H3 cell line induced by DNP24-BSA, thapsigargin or ionomycin. In addition, marmin suppressed 45Ca2+ influx on RBL-2H3 cell line induced by thapsigargin. Marmin also succeeded to inhibit the histamine release from RPMCs induced by thapsigargin. However, marmin showed weak inhibitory effects on the histamine release from RPMCs induced by compound 48/80, PMA or ionomycin. Based on the results, the inhibitory effect of marmin on the histamine release from mast cells high depends on the type of mast cell and also involves mechanisms related to intracellular Ca2+ signaling events by blocking Ca2+ influx into mast cells.

References

Bienenstock J, Tomioka M, Stead R, Ernst

P, Jordana M, Gauldie J, Dolovich J,

Denburg J. Modulation of human basophil

histamine release by protein kinase C

inhibitors differs with secretagogue and

with inhibitor. Am Rev Respir Dis.

;135(6 Pt 2):S5-8.

Galli SJ. New Concepts about the Mast

Cell. N Engl J Med. 1993;328:257–265 .

Rang HP, Dale MM, Ritter JM, Moore PK.

Pharmacology. 5th Ed. Churchill

Livingstone, Sydney. 2003;pp. 227-229.

Metcalfe DD, Baram D, Mekori YA. Mast

Cells. Physiol Rev. 1997;77(4):1033-1064.

Clark AM. Natural products as a resource

for new drugs. Pharm Res.

;13(8):1133-1144.

Schuster BG. A new integrated program for

natural product development and the value

of an ethnomedical approach. J Altern

Complement Med. 2001;7 Suppl 1:S61-72.

Gullo VP, McAlpine J, Lam KS, Baker D,

Petersen F. Drug discovery from natural

products. J Ind Microbiol Biotechnol.

;33(7):523-531.

Lampronti I, Martello D, Bianchi N,

Borgatti M, Lambertini E, Piva R, Jabbar S,

Choudhuri MS, Khan MT, Gambari R. In

vitro antiproliferative effects on human

tumor cell lines of extracts from the

Bangladeshi medicinal plant Aegle

marmelos Correa. Phytomedicine.

;10(4):300-308.

Arul V, Miyazaki S. Dhananjayan RJ.

Studies on the anti-inflammatory,

antipyretic and analgesic properties of the

leaves of Aegle marmelos Corr.

Ethnopharmacol. 2005;96(1-2):159-163.

Upadhya S, Shanbhag KK, Suneetha G,

Balachandra Naidu M, Upadhya S. A study

of hypoglycemic and antioxidant activity of

Aegle marmelos in alloxan induced diabetic

rats. Indian J Physiol Pharmacol.

;48(4):476-480.

Rana BK, Singh UP, Taneja V. Antifungal

activity and kinetics of inhibition by

essential oil isolated from leaves of Aegle

marmelos. J Ethnopharmacol.

;57(1):29-34.

Badam L, Bedekar SS, Sonawane KB,

Joshi SP. In vitro antiviral activity of bael

(Aegle marmelos, Corr) upon human

coxsackie viruses B1-B6. J Commun Dis.

;34(2):88-99.

Sachdewa A, Raina D, Srivastava AK,

Khemani LD. Effect of Aegle marmelos

and Hibiscus rosa-sinensis leaf extract on

glucose tolerance in glucose induced

hyperglycemic rats (Charles foster). J

Environ Biol. 2001;22(1):53-75.

Sabu MC, Kuttan R. Antidiabetic activity

of Aegle marmelos and its relationship with

its antioxidant properties. Indian J Physiol

Pharmacol. 2004;48(1):81-88.

Shoeb A, Kapil RS, Popli SP. Antidiabetic

activity of Aegle marmelos and its

relationship with its antioxidant properties.

Phytochemistry. 1973;12:2071-2073.

Basu D, Sen R. Alkaloids and Coumarins

from Root-Bark of Aegle marmelos.

Phytochemistry. 1974;13:2330-2330.

Srivastava SD, Srivastava S, Srivastava SK.

New Anthraquinones from The Heartwood

of Aegle marmelos. Fitoterapia.

;67(1):83-84.

Riyanto S. Phytochemical Studies and

Bioactivity Tests of Murraya paniculata

Jack, Aegle marmelos Correa, and Zingiber

amaricans Blume. Dissertation. Universiti

Putra Malaysia, 2003.

Barsumian EL, Isersky C, Petrino MG,

Siraganian RP. IgE-induced histamine

release from rat basophilic leukemia cell

lines. Eur J Immunol. 1981;11(4): 317-323.

Yamatodani A, Fukuda H, Wada H, Iwaeda

T, Watanabe T. High-performance liquid

chromatographic determination of plasma

and brain histamine without previous

purification of biological samples : cationexchange chromatography coupled with

post-column derivatization fluorometry. J

Chromatogr. 1985;344:115-123.

Patkar SA, Rasmussen U, Diamant B. On

the mechanism of histamine release induced

by thapsigargin from Thapsia garganica L.

Agents Actions. 1979;9(1):53-57.

Brayden DJ, Hanley MR, Thastrup O,

Cuthbert AW. Thapsigargin, a new

calcium-dependent epithelial anion

secretagogue. Br J Pharmacol.

;98(3):809-816.

Assem ES, Chong EK. Profiles of Different

Anti-allergy Effects of a New Histamine

Antagonist, BM 15,100 [proceedings]. Br J

Pharmacol. 1976;57(3):437-438.

Ryu SY, Kou NY, Choi HS, Ryu H, Kim

TS, Kim KM. Cnidicin, a Coumarin, from

the Root of Angelica koreana, Inhibits the

Degranulation of Mast Cell and the NO

Generation in RAW 264.7 Cells, Planta

Med. 2001;67(2):172-174.

Watanabe J, Shinmoto H, Tsushida T.

Coumarin and flavone derivatives from

estragon and thyme as inhibitors of

chemical mediator release from RBL-2H3

cells, Biosci. Biotechnol. Biochem.

;69(1):1-6.

Takase H, Yamamoto K, Hirano H, Saito

Y, Yamashita A. Pharmacological profile of

gastric mucosal protection by marmin and

nobiletin from a traditional herbal medicine,

Aurantii fructus immaturus. Jpn J

Pharmacol. 1994;66(1):139-147.

Liu FT, Bohn JW, Ferry EL, Yamamoto H,

Molinaro CA, Sherman LA, Klinman NR,

Katz DH. Monoclonal dinitrophenylspecific murine IgE antibody: preparation,

isolation, and characterization. J Immunol.

;124(6):2728-2737.

Orida N, Feldman JD, Katz DH, Liu FT.

IgE-mediated chemotaxis of rat basophilic

leukemia cells towards specific antigen. J

Exp Med. 1983;157(6):2166-2171.

Huang Y, Putney JW Jr. Relationship

between intracellular calcium store

depletion and calcium release-activated

calcium current in a mast cell line (RBL-1).

J Biol Chem. 1998;273(31):1954-1959.

Ali H, Maeyama K, Sagi-Eisenberg R,

Beaven MA. Antigen and thapsigargin

promote influx of Ca2+ in rat basophilic

RBL-2H3 cells by ostensibly similar

mechanisms that allow filling of inositol

,4,5-trisphosphate-sensitive and

mitochondrial Ca2+ stores. Biochem J.

;304(Pt 2):431-440.

Truneh A, Ennis M, Pearce FL. Some

characteristics of histamine secretion from

mast cells treated with ionomycin. Int Arch

Allergy Appl Immunol. 1982;69(1):86-88.

Barrett KE, Pearce FL. A comparison of

histamine secretion from isolated peritoneal

mast cells of the mouse and rat, Int Arch

Allergy Appl Immunol. 1983;72(3):234-

Ennis M, Truneh A, White JR, Pearce FL.

Calcium pools involved in histamine release

from rat mast cells. Int Arch Allergy Appl

Immunol. 1980;62(4):467-471.

Mousli M, Bronner C, Landry Y, Bockaert

J, Rouot B. Direct activation of GTPbinding regulatory proteins (G-proteins) by

substance P and compound 48/80. FEBS

Lett. 1990;259(2):260-262.

Palomaki VA, Laitinen JT. The basic

secretagogue compound 48/80 activates G

proteins indirectly via stimulation of

phospholipase D-lysophosphatidic acid

receptor axis and 5-HT1A receptors in rat

brain sections. Br J Pharmacol.

;147(6):596-606.

Bergstrand H, Lundquist B, Karabelas K,

Michelsen P. Modulation of human

basophil histamine release by protein kinase

C inhibitors differs with secretagogue and

with inhibitor. J Pharmacol Exp Ther.

;260:1028-1037.

Okano Y, Yamada K, Takagi H, Nozawa

Y. Biphasic effect of phorbol myristate

acetate on histamine secretion induced by

compound 48/80 in rat peritoneal mast

cells. Biochem Biophys Res Commun.

;137:1112-1118.

Okano Y, Takagi H, Nakashima S,

Tohmatsu T, Nozawa Y. Inhibitory action

of phorbol myristate acetate on histamine

secretion and polyphosphoinositide

turnover induced by compound 48/80 in

mast cells. Biochem Biophys Res Commun.

;132:110-117.

Katakami Y, Kaibuchi K, Sawamura M,

Takai Y, Nishizuka Y. Biochem Biophys

Res Commun. 1984;121:573-578.

Chakravarty N. The role of protein kinase

C in the histamine secretion from mast cell.

Acta Physiol Scand. 1990;139:319-331.

Shin TY. Stachys riederi inhibits mast cellmediated acute and chronic allergic

reactions. Immunopharmacol

Immunotoxicol. 2004;26:621-630.

Shin TY, Kim SH, Suk K, Ha JH, Kim I,

Lee MG, Jun CD, Kim SY, Lim JP, Eun JS,

Shin HY, Kim HM. Anti-allergic effects of

Lycopus lucidus on mast cell-mediated

allergy model. Toxicol Appl Pharmacol.

;209:255-262.

Kim SH, Choi CH, Kim SY, Eun JS, Shin

TY. Anti-allergic effects of Artemisia

iwayomogi on mast cell-mediated allergy

model. Exp Biol Med. 2005;230:82-88.

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Published

31-03-2011

How to Cite

1.
Agung Endro Nugroho, Sugeng Riyanto, Mohamad Aspollah Sukari, Kazutaka Maeyama. Anti-allergic effects of Marmin, a coumarine isolated from Aegle marmelos Correa: In vitro study. ijp [Internet]. 2011 Mar. 31 [cited 2024 Dec. 6];3(1):84-97. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/83

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