Anti-diabetic and acute toxicity studies of Annona squamosa L. ethanolic leaves extract.


  • Farah - Saeed Department of Pharmacognosy, Dow College of Pharmacy, Dow University of Health Sciences, Ojha Campus, Karachi-Pakistan
  • Mansoor Ahmad Research Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Karachi-Pakistan


Alloxan, Glibenclaimide, custard apple, hypoglycaemic, toxic effects


Aim of this research work was to explore the anti-diabetic activity and acute toxicity of Annona squamosa L. leaves ethanolic extract in albino rats and mice respectively. Diabetes was induced by Alloxan (120 mg/kg). Seven rats were taken in each group. Glibenclaimide (0.25 mg/kg) was taken as the standard drug. A. squamosa was administered orally in 100mg, 200mg and 400mg doses in three different groups of diabetes- induced rats. A. squamosa leaves extracts were found to have significant anti-diabetic activity. Acute toxicity study was carried out on administration of 800mg/kg, 1600mg/kg and 5000 mg/kg body weight. No acute toxicity was observed at 800mg/kg and 1600mg/kg doses. At 5000mg/kg body weight dose 100% fatality was recorded within 24 hours. Our research work revealed the safe and effective anti-diabetic activity of Annona squamosa ethanolic leaves extract.


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How to Cite

Farah - Saeed, Mansoor Ahmad. Anti-diabetic and acute toxicity studies of Annona squamosa L. ethanolic leaves extract. ijp [Internet]. 2017 Dec. 31 [cited 2023 Nov. 30];9(4):642-7. Available from:



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