Phytochemical Screening and Biological Studies of Shilajit (Asphaltum)


  • Shahid Aziz Department of Chemistry, Mirpur University of Science & Technology (MUST), Mirpur, Mirpur-10250 (AJK), Pakistan
  • Sidra Khaliq Department of Chemistry, Mirpur University of Science & Technology (MUST), Mirpur, Mirpur-10250 (AJK), Pakistan
  • Habib ur-Rehman Department of Chemistry, Mirpur University of Science & Technology (MUST), Mirpur, Mirpur-10250 (AJK), Pakistan
  • Kh. Shakeel Ghani Department of Chemistry, Mirpur University of Science & Technology (MUST), Mirpur, Mirpur-10250 (AJK), Pakistan
  • Muhammad Irshad Mohtarama Benazir Bhutto Shaheed Medical College, Pirpur, AJk, Pakistan
  • Ivan R Green Department of Chemistry and Polymer Science, University of Stellenbosch, P/Bag X1 Matieland 7602, South Africa
  • Hidayat Hussain UoN Chair of Oman’s Medicinal Plants and Marine Natural Products University of Nizwa P.O Box 33, Postal Code 616 Birkat Al Mauz, Nizwa, Sultanate of Oman


Shilajit, phytochemical screening, anti-microbial activity


Shilajit (asphaltum) is produced by the long term humification of dead plant material and organic vegetable matter by different micro-organisms and has great potential for the treatment of a variety of human conditions. This treatise reviews its origin, sources, chemical composition, biological and commercial importance. Phytochemical analysis was done by standard methods to evaluate different Shilajit (asphaltum) classes of compounds in different samples of shilajit which are responsible for their biological activity. Shilajit`s anti-microbial activity has been evaluated against four different bacterial strains viz., Escherichia coli, Psuedomonas aeuroginosa, Klebisella pneumonia and Staphylococcus aureus. Phytochemical analysis illustrated that shilajit contains terpenoids, cardiac glycosides, saponins and reducing sugars. Surprisingly, some classes of compounds are absent in shilajit viz., alkaloids, flavonoids, tannins and anthraquinones. . Shilajit showed no response towards halophytic bacteria and negligible activity was shown towards other strains of bacteria. Since anti-microbial activity is based on environmental factors its activity varied between locations.


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How to Cite

Shahid Aziz, Sidra Khaliq, Habib ur-Rehman, Kh. Shakeel Ghani, Muhammad Irshad, Ivan R Green, Hidayat Hussain. Phytochemical Screening and Biological Studies of Shilajit (Asphaltum). ijp [Internet]. 2017 Mar. 31 [cited 2024 Jun. 23];9(1):15-9. Available from:



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