Nano-curcumin: A Potent Enhancer of Body Antioxidant System in Diabetic Mice.


  • Nitin Dadasaheb Potphode Department of Zoology, Shivaji University, Kolhapur, (MS), India-416004
  • Jitesh Ankush Daunde Department of Zoology, Shivaji University, Kolhapur, (MS), India-416004
  • Sneha Sampatrao Desai Department of Zoology, Shivaji University, Kolhapur, (MS), India-416004
  • Madhuri Vasant Walvekar Department of Zoology, Shivaji University, Kolhapur, (MS), India-416004


Nano-curcumin, Antioxidative enzymes, Pancreas, oxidative stress, ROS


Nano preparation of drug to be helpful in targeted delivery, which avoids any unwanted damage of adjacent healthy tissues. Antidiabetic compounds from natural and synthetic sources have been found to successful management of diabetes. Antioxidants are compound that protect cell against the damaging effects of reactive oxygen species (ROS). Curcumin has many beneficial effects against health problems; it has limited use due to its poor bioavailability as concluded by number of its pharmacokinetic studies. Since the aim of this study was to investigate the effects of curcumin nanoparticles (Nano-curcumin) on antioxidative enzymes i.e Glutathione peroxidase (GPx), Superoxide dismutase (SOD) and Catalase (CAT) in pancreas of diabetic mice. For the present investigation mice (Mus musculus) used as experimental animal. Mice were divided into four groups viz, a) Control group b) Diabetic group c) Recovery group I- Diabetic mice treated with curcumin d) Recovery group II - Diabetic mice treated with curcumin and nano-curcumin. The activity of antioxidative enzymes in the pancreas was recorded at the end of experiment. There was decrease in antioxidative enzymes in pancreas of diabetic mice compared to control. After the treatment of curcumin and curcumin nanoparticles significant increase in levels of antioxidative enzymes in recovery group I and II was observed. Moreover as compare to free curcumin nano-curcumin showed better results in enhancement of antioxidative enzymes. Thus it proves that nano-curcumin found to be potent antioxidative compound to reduced oxidative stress induced during the diabetes.


Bansal S, Goel M, Aqil F, Vadhanam M, Gupta R. Advanced drug delivery systems of curcumin for cancer chemoprevention. Cancer Prev Res. 2011(4); 1158-1171.

Pandey M, Kumar S, Thimmulappa R, Parmar V, Biswal S, Watterson A. Design synthesis and evaluation of novel PEGylated curcumin analogs as potent Nrf2 activators in human bronchial epithelial cells. Eur J Pharm Sci. 2011(43); 16-24.

Tolman K, Fonseca V, Dalpiaz A. Tan M Spectrum of Liver Disease in Type 2 Diabetes and Management of Patients with Diabetes and Liver Disease. Diabetes care. 2007; 30(3); 734-743.

Makadia S, Siegel. Poly lactic-co-glycolic acid (PLGA) as biodegradable controlled drug delivery carrier. Polymer 2011(3); 1377-1397.

Sah E, Sah H. Recent trends in preparation of poly (lactide-co-glycolide) nanoparticles by mixing polymeric organic solution with antisolvent. J. Nanomater. 2015; 16(61).

Gouaref I, Detaille D, Wiernsperger N, Khan N, Leverve X, Koceir E. The desert gerbil Psammomys obesus as a model for metformin-sensitive nutritional type 2 diabetes to protect hepatocellular metabolic damage: Impact of mitochondrial redox state. PLoS ONE. 2017; 12(2).

King H, Aubert R, Herman W. Global Burden of Diabetes 1995-2025: prevalence numerical estimates and projections. Diabetes Care. 1998; 21(9); 1414-1431.

Ihara S, Toyokuni K, Uchida H, Odaka T, Tanaka H, Ikeda H, Hiai Y, Seino, Yamada Y. Hyperglycemia causes oxidative stress in pancreatic beta-cells of GK rats, a model of type 2 diabetes. Diabetes. 1999(48); 927–932.

Baynes JW. Role of oxidative stress in development of complications in diabetes. Diabetes 1991; 40(4); 405–412.

Nishikawa T, Edelstein D, Du XL, Yamagishi S, Matsumura T, Kaneda Y, Yorek MA, Beebe D, Oates PJ, Hammes HP, Giardino I, and Brownlee M. Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature. 2000(404); 787–790.

Tiedge M, Lortz S, Drinkgern J, Lenzen S. Relation between antioxidant enzyme gene expression and Antioxidative defense status of insulin-producing cells. Diabetes. 1997(46); 1733–1742.

Hunt T, Smith, Wolff S. Autoxidative glycosylation and possible involvement of peroxides and free radicals in LDL modification by glucose. Diabetes. 1990; 39(11); 1420–1424.

Forbes J, Coughlan M, Cooper M. Oxidative stress as a major culprit in kidney disease in diabetes. Diabetes. 2008; 57(6); 446–1454.

Wolff S, Dean R. Glucose autoxidation and protein modification: the potential role of ‘autoxidative glycosylation’ in diabetes. Biochemical Journal. 1987; 245(1); 243–250.

American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2006; 29(1); 43–48.

Mansour H, Newairy A, Yousef M, Sheweita S. Biochemical study on the effects of some Egyptianherbs in alloxan-induced diabetic rats. Toxicology. 2002; 170(3); 221–228.

Cade WT. Diabetes-related microvascular and macrovascular diseases in the physical therapy setting. Phys. Ther. 2008; 88(11); 1322– 1335.

Lupachyk S, Watcho P, Stavniichuk R, Shevalye H, Obrosova IG. Endoplasmic reticulum stress plays a key role in the pathogenesis of diabetic peripheral neuropathy. Diabetes. 2013; 62(3); 944–952.

Cameron NE, Cotter MA. The relationship of vascular changes to metabolic factors in diabetes mellitus and their role in the development of peripheral nerve complications. Diabetes. 1994(10);189–224.

Soto C, Mena R, Luna J, Cerbon M, Larrieta E, Vital P, Uria E, Sanchez M, Recoba R, Barron H, Favri L, Lara A. Silymarin induces recovery of pancreatic function after alloxan damage in rats. Life Sci. 2004; 75(18); 2167–2180.

Winterbourn C, Munday R. Glutathione-mediated redox cycling of alloxan. Mechanisms of superoxide dismutase inhibition and of metal-catalyzed OH formation. Biochem. Pharmacol. 1989(38); 271–277.

Gorus FK, Malaisse WJ, Pipeleers DG. Alloxan selectively and rapidly accumulates in b-cell. Selective uptake of alloxan in pancreatic beta-cells. Biochemistry. 1982(208); 513–515.

Carroll PB, Moura AS, Rojas E, Atwater I. The diabetogenic agent alloxan increases K permeability by a mechanism involving action of ATP-sensitive K-channels in mouse pancreatic beta-cells. Mol. Cell. Biochem. 1994(140); 127–136.

Jacob RA. The integrated antioxidant system. Nutrition Research. 1995(15);755.

Pepato MT, Baviera AM, Vendramini RC, Perez MPMS, Kettelhut IC, Brunetti IL. Cissus Sicyoides (Princess Vine) in the long term treatment of streptozotocin diabetic rats. Biotechnol Appl Biochem. 2003(37); 15-20.

Loew D, Kaszkin M. Approaching the problem of bioequivalence of herbal medicinal products. Phytother Res. 2002(16); 705- 711.

Marles RJ, Farnsworth NR. Antidiabetic plants and their active Constituents. Phytomedicine. 1995(2); 133-189.

Braga ME, Leal PF, Carvalho JE, Meireles MAA. Comparison of yield composition and antioxidant activity of turmeric (Curcuma longa L.) extractsobtained using various techniques. J Agric Food Chem. 2003(51); 6604-6611.

Aggarwal BB, Anand P, Kunnumakkara AB, Newman RA. Bioavailability of curcumin: Problems and Promises.” Mol. Pharmaceutics. 2007 (4); 807-818.

Jaiswal J, Gupta S, Kretuter J. Preparation of biodegradable cyclosporine nanoparticles by high pressure emulsification solvent evaporation process. J. Control Release. 2004(96); 169-178.

Beauchamp C, Fridovich I. Superoxide dismutase improved assay and assay applicable to acrylamide gels. Anal. Biochem. 1971(44); 276.

Beers R, Sizer I. A spectrophotometer method for measuring the breakdown of hydrogen peroxide by GPx. J. Bio. Chem. 1952; 195(133).

Luck H. Catalase. In “Methods in enzymatic Analysis” 2 edited by Gergmeyer. Academic press New York. 1974; 885-894.

Wills ED. Mechanisms of lipid peroxide formation in animal tissue. J.Biochem. 1966(99); 667-676.

Kakkar R, Mantha S, Radhi J, Prasad K, Kalra J. Increased oxidative stress in rat liver and pancreas during progression of streptozotocin induced diabetes. Clin. Sci. 1998(94); 623–632.

Mohamed A, Bierhaus A, Schiekofer S. The role of oxidative stress and NF (B) activation in late diabetic complications. Biofactors. 1999(10);175–179.

Lenzen S. The mechanisms of alloxan- and streptozotocine induced diabetes. Diabetologia. 2008; 51(2); 216–226.

Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB. Biological activities of curcumin and its analogues (Congeners) made by man and mother Nature. Biochem pharmacol. 2008(76); 1590-1611.

Setthacheewakul S, Mahattanadul S, Phadoongsombut N, Pichayakorn W, Wiwattanapatapee R. Development and evaluation of self-microemulsifying liquid and pellet formulations of curcumin and absorption studies in rats. Eur J Pharm Biopharm. 2010(76); 475-485.

Aksoy N, Vural H, Sabuncu T, Arslan O, Aksoy S. Beneficial effects of vitamins C and E against oxidative stress in diabetic rats. Nutr. Re. 2005(25); 625–630.

Yu J, Cui P, Zeng W, Xie X, Liang W, Lin G, Zeng L. Protective effect of selenium-polysaccharides from the mycelia of Coprinus comatus on alloxan-induced oxidative stress in mice. Food Che. 2009(117); 42–47.

Daunde JA, Desai SS, Desai PJ, Kamble PS, Bhoi AV, Gaikwad PR, Walvekar MV. Nano-scaling of Trigonelline Improves Antioxidative Status of hfd-stz Induced Diabetic Mice. IJRASET. 2018; 6(2); 2547-2552.

Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic vascular complications. Diabetes Care. 1996(19); 257–267.

Goel A, Kunnumakkara AB, Aggarwal BB. Curcumin as "Curecumin": From kitchen to clinic. Biochemical Pharmacology. 2008; 75(4); 787-809.




How to Cite

Nitin Dadasaheb Potphode, Jitesh Ankush Daunde, Sneha Sampatrao Desai, Madhuri Vasant Walvekar. Nano-curcumin: A Potent Enhancer of Body Antioxidant System in Diabetic Mice. ijp [Internet]. 2018 Sep. 30 [cited 2023 Dec. 11];10(3):162-7. Available from:



Original Research Articles