Antioxidant, anti-cholinesterase, anti-α-glucosidase and prebiotic properties of beta-glucan extracted from Algerian barley

Authors

  • Saad Mebrek 1. Laboratory of Biotoxicology, Department of Biology, Faculty of Natural Sciences and Life, University of DjilaliLiabes, Sidi Bel Abbes 22000, Algeria 2. Biotechnology Research Centre, Ali Mendjli Nouvelle ville UV 03, Constantine 25000, Algeria
  • Hanene Djeghim 2. Biotechnology Research Centre, Ali Mendjli Nouvelle ville UV 03, Constantine 25000, Algeria
  • Yamina Mehdi Laboratory of Biotoxicology, Department of Biology, Faculty of Natural Sciences and Life, University of DjilaliLiabes, Sidi Bel Abbes 22000, Algeria Department of Biology, Institute of Sciences, Nourelbachir University Center, AL Bayadh 32000, Algeria
  • Asma Meghezzi Biotechnology Research Centre, Ali Mendjli Nouvelle ville UV 03, Constantine 25000, Algeria
  • Sirajudheen Anwar Department of Medicinal Chemistry, Pharmacology and Toxicology unit, College of Clinical Pharmacy Albaha University Saudi Arabia
  • Nasser A Ali Awadh Department of Pharmacognosy, College of Clinical Pharmacy, Albaha University, Albaha, Kingdom of SaudiArabia.
  • Mohammed Benali Laboratory of Biotoxicology, Department of Biology, Faculty of Natural Sciences and Life, University of DjilaliLiabes, Sidi Bel Abbes 22000, Algeria

Keywords:

β Glucan, antioxidant, anti-cholinesterase, anti-α-glucosidase and prebiotic

Abstract

Beta-glucan, such as barley-derived beta-glucan (BBG), are homopolysaccharides that have attracted attention by their nutritional and therapeutic properties. The aim of this study was to evaluate the antioxidant power of BBG extracted from local Algerian variety of barley (SAIDA 183), and its acetylcholinesterase, alpha glucosidase inhibitory activity as well as its prebiotic potential by fermentation with lactic acid bacteria isolated from camel’s milk, namely lactococcuslactisssplactis (Lc.l.l) and leuconostocmesenteroidesspmesenteroides (Ln.m.m). The results revealed that BBG exhibited low activity against DPPH and ferric-reducing power (IC50 4018.61 ± 656.69 and A0.5 at 359.88 ±63.64 µg/mL respectively), in contrast to other antioxidant tests (ABTS, Beta-carotene and CUPRAC) where BBG demonstrated a moderate activity (IC50 529.91 ±26.37, IC50 161.013±13.322, A0.5 529.79 ± 48.65 µg/mL). The scavenging ability of hydroxyl radical and superoxide radical by BBG with an IC50 at 2268.38±101.57 µg/mL and IC50 345.26± 62.32 µg/mL, respectively, while enzymatic inhibition by BBG exhibited for AChE at IC50 859.164 ±64.46 μg/mL , BChE at IC50 at 725.470 ±30.95 , α-Amylase inhibitory activity at IC50 2986.785 ± 37.046 . The bacterial growth of the two strains used in this study is favorably affected by the use of BBG as the only carbon source, in comparison with glucose as a control. In light of these findings, it can be concluded that BBG have shown moderate antioxidant and enzyme inhibitory activities and can be used as a prebiotic by acting synergistically with probiotics in functional food matrices.

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Published

31-03-2018

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1.
Saad Mebrek, Hanene Djeghim, Yamina Mehdi, Asma Meghezzi, Sirajudheen Anwar, Nasser A Ali Awadh, Mohammed Benali. Antioxidant, anti-cholinesterase, anti-α-glucosidase and prebiotic properties of beta-glucan extracted from Algerian barley. ijp [Internet]. 2018 Mar. 31 [cited 2024 May 19];10(1):58-67. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/590

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