In-vitro antioxidant, Xanthine oxidase-inhibitory and in-vivo Anti-inflammatory, analgesic, antipyretic activity of Onopordum acanthium


  • Sofiane Habibatni Laboratory of Development of Natural Resources and Synthesis of Bioactive Substances, Mentouri University, Constantine, Algeria 2, Department of Biology. University of Khenchel Abbas Laghrour, Kenchela , Algeria
  • Abdessalam Fatma Zohra Department of Biology. University of Khenchel Abbas Laghrour, Algeria
  • Hani Khalida Department of Biology. University of Khenchel Abbas Laghrour, Algeria
  • Sirajudheen Anwar Department of Pharmacology and Toxicology College of Clinical Pharmacy Albaha University Saudi Arabia
  • Iman Mansi Department of Clinical pharmacy and Pharmacy Practice, Faculty of Pharmaceutical sciences, The Hashemite University, P.O.Box 330127 Zarqa 13133 Jordan
  • Nasser A Awadh Ali Department of Pharmacognosy, College of Clinical Pharmacy, Albaha University, Albaha, KSA


Onopordum acanthium, Flavonoids, Xanthine oxidase inhibition, antinflammation, analgesic and antipyretic


Onopordum acanthium (Scotch thistle) belong to Asteraceae (Compositae). O. acanthium is a flowering biennial plant native to Europe and Western Asia with coarse spiny leaves 20-50 cm in width with conspicuous and spiny-winged stems. We have previously reported pro-apoptotic and cytotoxic effect of Onopordum acanthium crude extract against glioblastoma U-373 cells. The present study was designed to evaluate the cytotoxicity, antioxidant, xanthine oxidase inhibition, anti-inflammatory, analgesic, antipyretic activity of butanolic extract of Onopordum acanthium. Cytotoxicity of different solvent (methanolic, butanol, chloroform and petroleum ether) extract studied by brine shrimp lethality bioassay, total flavonoid and phenolic, antioxidant, xanthine oxidase inhibition activity was studied by in-vitro whereas anti- inflammation studied by carrageenan-induced paw edema model, antipyretic with 20 % brew yeast injection induced pyretic model, analgesic with 1 % acetic acid induced analgesic model investigated in in-vivo in wistar rats. Good antioxidant activity was found with IC50 = 134.4 µg/ml with considerable amount of total phenolic and flavonoid content. Xanthine oxidase inhibition effect was weak with IC50 = 572.9 µg/ml. Oral administration of O. Acanthium butanolic extract (OA) showed minimum lethality of brine shrimp nauplii henceforth OA butanolic phases was selected for further in-vivo studies. OA 200 and 400 mg/kg body weight decreased the oedema by 37.78 % and 40.52 %, respectively; standard aspirin 100 mg/kg decreased 42.62 % at 5th hour of Carrageenan injection. OA 200 and 400 mg/kg significantly decreased acetic acid-induced abdominal writhes when compared to standard aspirin. OA have shown dose and time dependent decrease in body temperature in yeast induced pyrexia, comparable to standard, aspirin. The present results demonstrate that OA has notable anti-inflammatory, antipyretic, analgesic activity related to presence of phenolic compounds as from literature it has been demonstrated that isolated compounds from aerial parts of Onopordum acanthium had strong activity in in-vitro assay.


. Rainsford KD, Whitehouse MW. Antiinflammatory/antipyretic salicylic acid esters with low gastric activity. Agents Action1980;10:451–5.

. Fiebich BL, Chrubasik S. Effects of an ethanolic Salix extract on the release of selected inflammatory mediators in vitro. Phytomedicine 2004;11:135–8.

. Khalilova AZ, Litvinov IA, Beskrovnyi DV, Gubaidullin AT, Shakurova ER, Nuriev IR, Khalilov LM, Dzhemilev UM. Isolation and crystal structure of taraxasteryl acetate from Onopordum acanthium. Chem Nat Comp 2004; 40: 254

. Eisenman SW, Zaurov DE, Struwe L. Medicinal Plants of Central Asia: Uzbekistan and Kyrgyzstan. New York: Springer; 2013: 178.

. Bruno M, Maggio A, Rosselli S, Safder M, Bancheva S. The metabolites of the genus Onopordum (Asteraceae): Chemistry and biological properties. Curr Org Chem 2011; 15: 888–927.

. Kiselova Y, Ivanova D, Chervenkov T, Gerova D, Galunska B, Yankova T. Correlation between the in vitro antioxidant activity and polyphenol content of aqueous extracts from Bulgarian herbs. Phytother Res 2006; 20: 961–965

. Abuharfeil NM, Maraqa A, Von Kleist S. Augmentation of natural killer cell activity in vitro against tumor cells by wild plants from Jordan. J Ethnopharmacol 2000; 71: 55–63.

. Csupor-Löffler B, Hajdú Z, Rethy B, Zupkó I, Máthé I, Rédei T, Falkay G, Hohmann J. Antiproliferative activity of Hungarian Asteraceae species against human cancer cell lines, Part II. Phytother Res 2009; 23:1109–1115.

. Csupor-Löffler B, Zupkó I, Molnár J, Forgo P, Hohmann J. Bioactivity guided isolation of antiproliferative compounds from the roots of Onopordum acanthium. Nat Prod Commun 2014; 9: 337–340.

. Abusamra YA, 2015. Evaluation of putative cytotoxic activity of crude extracts from Onopordum acanthium leaves and Spartium junceum flowers against the U-373 glioblastoma cell line. Pak J Pharm Sci 2015;28(4):1225-32.

. Lajter I, Pan SP, Nikles S, Ortmann S, Vasas A, Csupor-Löffler B, Forgó et al. Inhibition of COX-2 and NF-κB1 Gene Expression, NO Production, 5-LOX, and COX-1 and COX-2 Enzymes by Extracts and Constituents of Onopordum acanthium. Planta Med 2015; 81(14):1270-6.

. Anwar S , Desai S, Mandlik R. Exploring Antidiabetic Mechanisms of Action of Galactomannan: A Carbohydrate Isolated from Fenugreek Seeds. J Complement Integrat Med 2009;6:16.

.Anwar S, Desai S, Eidi M, Eidi A. (2011). Antidiabetic activitiesof Fenugreek (Trigonella foenum-graecum) seeds. In: V. R. Preedy, R. R. Watson, V.- 2 -B. Patel. Nuts and Seeds in Health and Disease Prevention. p. 461-478, London: Elsevier, ISBN/ISSN: 9780123756886.

. Anwar S, Crouch RA, Awadh Ali NA, Al-Fatimi MA, Setzer WN, Wessjohann L. Hierarchical cluster analysis and chemical characterisation of Myrtus communis L. essential oil from Yemen region and its antimicrobial, antioxidant and anti-colorectal adenocarcinoma properties. Nat Prod Res 2017 ;1-6.

. Meyer BN, Ferrigni NR, Putnam JE, Jacobson LB, Nichols DE, McLaughlin JL. Brine shrimp: Aconvenient general bioassay for active plant constituents. Planta Med. 1982; 45 (5): 31-34.

. Habibatni S, Miceli N, Ginestra G, Maameri Z, Bisignano C, Cacciola F, Utczas M, Mondello L, S Anwar, Benayache S, Atrouz D, Benayache F, Taviano MF. Antioxidant and antibacterial activity of extract and phases from stems of Spartium Junceum L. growing in Algeria. Int J of Phytomed 2016; 8: 37-46.

. Jothy SL, Aziz A., Chen Y., and Sasidharan S., Antioxidant Activity and Hepatoprotective Potential of Polyalthia longifolia and Cassia spectabilis Leaves against Paracetamol-Induced Liver Injury, Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 561284, 10 pages, 2012. doi:10.1155/2012/561284

. Singleton, V.L.; Orthofer, R.; Lamuela-Raventos, R.M. 1999, Analysis of total phenols and other, oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Meth. Enzymol, 299, 152–178.

. Samaha HAM, Ali NAA, Mansi I, Abu-El-Halawa R . Antimicrobial, antiradical and xanthinoxidase inhibitory activities of Juniperus procera plant extracts from Albaha. World J. of pharmacy and pharmaceutical sciences 2017; 6: 232-242.

.Adeyemi OO, Okpo SO, and Ogunti OO. Analgesic and anti-inflammatory effects of the aqueous extract of leaves of Persea americana Mill (Lauraceae). Fitoterapia 2002;7: 375–380.

.Fezai M, Senovilla L, Jemaa M, Ben-Attia M. Analgesic, anti-inflammatory and anticancer activities of extra virgin olive oil. J Lipids. 2013;2013:129736

.Afsar T, Khan MR, Razak S, Ullah S, Mirza B. Antipyretic, anti-inflammatory and analgesic activity of Acacia hydaspica R. Parker and its phytochemical analysis. BMC Complement Altern Med. 2015 Apr 29;15:136.

. M.A. Soobrattee, V.S. Neergheen, A. Luximon-Ramma, O.I. Aruoma, O.T. Bahorun, Phenolics as potential antioxidant therapeutic agents: mechanism and actions, Mutat. Res. Fundam. Mol 2005; 579:200–213.

. Speciale, A., Anwar, S., Ricciardi, E., Chirafisi, J. et al., Cellular adaptive response to glutathione depletion modulates endothelial dysfunction triggered by TNF-α. Toxicol. Lett. 2011;207:291–297.

. Speciale A1, Anwar S, Canali R, Chirafisi J, Saija A, Virgili F, Cimino F. Cyanidin-3-O-glucoside counters the response to TNF-alpha of endothelial cells by activating Nrf2 pathway. Mol Nutr Food Res. 2013 ;57(11):1979-87. doi: 10.1002/mnfr.201300102.

. Manandhar S, You A, Lee ES, Kim JA, Kwak MK. Activation of the Nrf2-antioxidant system by a novel cyclooxygenase-2 inhibitor furan-2-yl-3-pyridin-2-yl-propenone: implication in anti-inflammatory function by Nrf2 activator. J Pharm Pharmacol. 2008; 60(7):879-87. doi: 10.1211/jpp.60.7.0009.

.Anwar S, Fratantonio D, Ferrari D, Saija A, Cimino F, Speciale A. Berry anthocyanins reduce proliferation of human colorectal carcinoma cells by inducing caspase-3 activation and p21 upregulation. Mol Med Rep. 2016;14(2):1397-403. doi: 10.3892/mmr.2016.5397.

. Anwar S, Speciale A, Fratantonio D, Cristani M, Saija A, Virgili F, Cimino F. Cyanidin-3-O-glucoside modulates intracellular redox status and prevents HIF-1 stabilization in endothelial cells in vitro exposed to chronic hypoxia. Toxicol Lett. 2014;226(2):206-13. doi: 10.1016/j.toxlet.2014.01.048.

.Richette P, Bardin T . Gout. Lancet 2010; 375 (9711): 318–28.

. Blokhina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot. 2003;91(2):179–94.

. Granger DN.Role of xanthine oxidase and granulocytes in ischemia reperfusion injury. Am J Physiol Heart Circ Physiol 1988; 255(6):H1269–75.

.Duarte I, Nakamura M, Ferreira S. Participation of the sympathetic system in acetic acid-induced writhing in mice. Braz J Med Biol Res 1987;21(2):341–3.

. Fenton H. LXXIII. —Oxidation of tartaric acid in presence of iron. Transactions. 1894; 65:899–910.

.Scandalios JG. Genomic responses to oxidative stress. Molecular Medicine: Encyclopedia of Molecular Cell Biology. 2004;5(2):489–512.

. Muhammad N, Saeed M, Khan H. Antipyretic, analgesic and anti-inflammatory activity of Viola betonicifolia whole plant. BMC Complement Altern Med. 2012;12(1):59.

. Khan MA, Khan H, Tariq SA, Pervez S. Urease Inhibitory Activity of Aerial Parts of Artemisia scoparia: Exploration in an In Vitro Study. Ulcers. 2014:184736.




How to Cite

Sofiane Habibatni, Abdessalam Fatma Zohra, Hani Khalida, Sirajudheen Anwar, Iman Mansi, Nasser A Awadh Ali. In-vitro antioxidant, Xanthine oxidase-inhibitory and in-vivo Anti-inflammatory, analgesic, antipyretic activity of Onopordum acanthium. ijp [Internet]. 2017 Mar. 31 [cited 2024 Jun. 23];9(1):92-100. Available from:



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