In-vitro antioxidant, Xanthine oxidase-inhibitory and in-vivo Anti-inflammatory, analgesic, antipyretic activity of Onopordum acanthium

Authors

  • Sofiane Habibatni Laboratory of Development of Natural Resources and Synthesis of Bioactive Substances, Mentouri University, Constantine, Algeria 2, Department of Biology. University of Khenchel Abbas Laghrour, Kenchela , Algeria
  • Abdessalam Fatma Zohra Department of Biology. University of Khenchel Abbas Laghrour, Algeria
  • Hani Khalida Department of Biology. University of Khenchel Abbas Laghrour, Algeria
  • Sirajudheen Anwar Department of Pharmacology and Toxicology College of Clinical Pharmacy Albaha University Saudi Arabia
  • Iman Mansi Department of Clinical pharmacy and Pharmacy Practice, Faculty of Pharmaceutical sciences, The Hashemite University, P.O.Box 330127 Zarqa 13133 Jordan
  • Nasser A Awadh Ali Department of Pharmacognosy, College of Clinical Pharmacy, Albaha University, Albaha, KSA

Keywords:

Onopordum acanthium, Flavonoids, Xanthine oxidase inhibition, antinflammation, analgesic and antipyretic

Abstract

Onopordum acanthium (Scotch thistle) belong to Asteraceae (Compositae). O. acanthium is a flowering biennial plant native to Europe and Western Asia with coarse spiny leaves 20-50 cm in width with conspicuous and spiny-winged stems. We have previously reported pro-apoptotic and cytotoxic effect of Onopordum acanthium crude extract against glioblastoma U-373 cells. The present study was designed to evaluate the cytotoxicity, antioxidant, xanthine oxidase inhibition, anti-inflammatory, analgesic, antipyretic activity of butanolic extract of Onopordum acanthium. Cytotoxicity of different solvent (methanolic, butanol, chloroform and petroleum ether) extract studied by brine shrimp lethality bioassay, total flavonoid and phenolic, antioxidant, xanthine oxidase inhibition activity was studied by in-vitro whereas anti- inflammation studied by carrageenan-induced paw edema model, antipyretic with 20 % brew yeast injection induced pyretic model, analgesic with 1 % acetic acid induced analgesic model investigated in in-vivo in wistar rats. Good antioxidant activity was found with IC50 = 134.4 µg/ml with considerable amount of total phenolic and flavonoid content. Xanthine oxidase inhibition effect was weak with IC50 = 572.9 µg/ml. Oral administration of O. Acanthium butanolic extract (OA) showed minimum lethality of brine shrimp nauplii henceforth OA butanolic phases was selected for further in-vivo studies. OA 200 and 400 mg/kg body weight decreased the oedema by 37.78 % and 40.52 %, respectively; standard aspirin 100 mg/kg decreased 42.62 % at 5th hour of Carrageenan injection. OA 200 and 400 mg/kg significantly decreased acetic acid-induced abdominal writhes when compared to standard aspirin. OA have shown dose and time dependent decrease in body temperature in yeast induced pyrexia, comparable to standard, aspirin. The present results demonstrate that OA has notable anti-inflammatory, antipyretic, analgesic activity related to presence of phenolic compounds as from literature it has been demonstrated that isolated compounds from aerial parts of Onopordum acanthium had strong activity in in-vitro assay.

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Published

31-03-2017

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1.
Sofiane Habibatni, Abdessalam Fatma Zohra, Hani Khalida, Sirajudheen Anwar, Iman Mansi, Nasser A Awadh Ali. In-vitro antioxidant, Xanthine oxidase-inhibitory and in-vivo Anti-inflammatory, analgesic, antipyretic activity of Onopordum acanthium. ijp [Internet]. 2017 Mar. 31 [cited 2024 Dec. 5];9(1):92-100. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/517

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