Identification of alkaloids in methanol extract from leaves of Semenovia suffruticosa when humic substances were added to its root soil in the primary growth
Keywords:Crude alkaloid solution, Sapropel-solvent water, GC-MS technique, Pyrimido [1, 2-a] quinoxalinone, , 3, 8-triisopropyl-6-methyl-2, 7-naphthyridine
Alkaloids are a group of natural compounds originally found in plants. Suffruticosa is a species of the genus Semenovia, the Apiaceous family, a native herb in Iran. In this family, alkaloids are found rarely. Humic substances application to the root soil at the beginning of plant growth improves quality, productivity and physical properties of the soil. Accordingly, humic substances were added separately to the root soil of the samples and other samples were selected as the blank plant sample in the distance of 50 cm. Methanol extracts were prepared from dried and powdered leaf samples. The crude alkaloid solution was extracted from methanol extract with 2 % sulfuric acid, dense ammonia and chloroform. The crude alkaloid solution was passed gradiently with mixture solvents of methanol and chloroform through a chromatography column. The output samples of the chromatography column were analyzed by FT-IR, UV-Vis, and TLC and GC-MS techniques. Pyrimido [1, 2-a] quinoxalinone and 1, 3,8-triisopropyl-6-methyl-2,7-naphthyridine alkaloids were identified in both samples and the extracted humic acid (0.513% and 0.979% respectively) and the Sapropel solution (0.449% and 0.678% respectively )were added to their plants soil. Also another alkaloid named 9-benzyloxypyrido [1,2-a]pyrimidin-4-one(9.538%) was identified in the sample and Sapropel was sprayed onto its root soil. No alkaloid was found in the blank plant sample. The results showed that humic substances had considerable effects on the effective substances of the plant Semenovia.suffruticosa.
.Rechinger KH .Semenvia, Johreniopsis, Bunium, Smyrnium. In: Flora Ironical Umbellifera. 241 Edits Austria.1987;162:456-486.
.Mozaffarian V.Flora of Iran; family Apiaceae, Research Institute of Forests and Rangelands, Tehran.2007:p. 477.
.Pfeifer T, Klaus U,Hoffmann R,Spiteller M.Characterisation of humic substances using atmospheric pressure chemical ionisation and electrospray ionisation mass spectrometry combined with size-exclusion chromatography, Journal of Chromatography A.2001;926:151–159.
.Mayhew L. Humic substances in biological agriculture. journal of ACRES .2004;Vol. 34, No. 1:2.
.Bernal MP,Paredes C, Sanchez-Monedero MA and Cegarra J.Maturity and stability parameters of composts prepared with a wide range of organic wastes. Bioresource
.Gadimov A, Ahmaedova N and Alieva RC. Symbiosis nodules bacteria Rhizobium legominosarum with peas (Pisum sativum) nitrate reductase, salinification and potassium humate. Azerbaijan National Academy of Sciences, Azerbaijan. 2007.
.Yang CM,Wang MH, Lu YF, Chang IF and Chou CH.Humic substances affect the activity of chlorophyllase. J. Chem. Ecol.2004; 30(5): 1057-1065.
.Vaughan D, Ord BG.Uptake and incorporation of 14C-labelled soil organic matter by roots of Pisum sativum L. J Exp Bot.1981; 32:679-87.
.Carletti P, Masi A, Spolaore B,Polverino De.Laureto P,De Zorzi M. Protein Expression Changes in Maize Roots in Response to Humic Substances. J Chem Ecol.2008;34:804-18.
.Schmidt W, Cesco S,Santi S,Pinton R, Varanini Z .Water-extractable humic substances as nutrient acquisition signals for root hairs development in Arabidopsis. In: Hartmann A, Schmid M, Wenzel W, Hinnsinger P eds. Rizosphere 2004-Perspectives and Challenges. Neuherberg: GSF-Berich,2005: 71.
.Zandonadi DB, Canellas LP, Façanha AR.Indolacetic and humic acids induce lateral root development through a concerted plasma lemma and tonoplast H+ pumps activation. Planta,2007; 225:1583-95.
.Nardi S,Muscolo A, Vaccaro S,Baiano S, Spaccini R, Piccolo A .Relationship between molecular characteristics of soil humic fractions and glycolytic pathway and Krebs cycle in maize seedlings. Soil Biol Biochem.2007; 39:3138-46.
.Wang S,Mulligan CN.Enhanced mobilization of arsenic and heavy metals from mine tailings by humic acid. Chemosphere.2009; 74:274-9.
.Janos P, Hula V, Bradnová P, Pilarová V, Sedlbauer V .J.Reduction and immobilization of hexavalent chromium with coal- and humate-based sorbent. Chemospher .2009; 75:732-8.
.Nardi S, Pizzeghello D,Muscolo A, Vianello A.Physiological effects of humic substances on higher plants. Soil Biol Biochem.2002; 34, 1527-36.
.Trevisan S,Francioso O,Quaggiotti S,Nardi S .Humic substances biological activity at the plant-soil interface. Plant Signaling & Behavior.2010;5, 6:635-643.
.Simpson AJ,Kingery WL,Hayes MH, Spraul M, Humpfer E,Dvortsak P. Molecular structures and associations of humic substances in the terrestrial environment. Naturwissenschaften.2002;89:84-8.
.Stevenson, FJ.Humus chemistry. Genesis, composition, reactions. 2nd ed. New York: John Wiley & Sons.1994.
.Schulten HR, Leinweber P.New insights into organicmineral particles: composition, properties, and models of molecular structure. Biol Fertil Soils.2002; 30:399-432.
.Senesi N, Loffredo E.Soil humic substances, In: Biopolymers. Lignin, humic substances and coal Vol 1. (Hofrichter, M. and Steinbuchel, A., ed.). 2001:247–99.
.Schulten H.R,Schnitzer M.A state-of-the-art structural concept for humic substances. Naturwissenchaften.1993; 80:29-30.
.Alvarez-Puebla RA, Goulet PJG, Garrido JJ.Characterization of the porous structure of different humic. Colloids Surf A, Physicochem Eng Asp.2005; 256:129-35.
.Eladia M, Méndez P, Havel J, Patočka J.Humic substances - compounds of still unknown structure: applications in agriculture, industry, environment, and biomedicine. Journal of Applied Biomedicen.2005; 3, 13: 42.
.Piccolo A, Celano G, Pietramellara G.Effects of fractions of coal-derived humic substances on seed germination and growth of seedlings (Lactuga sativa and Lycopersicum esculentum). Biol Fertil, Soil.1993; 16: 11-5.
.Quaggiotti S, Ruperti B, Pizzeghello D,Francioso O, Tugnoli V,Nardi S.Effect of low molecular size humic substances on the expression of genes involved in nitrate transport and reduction in maize (Zea mays L.). J Exp Bot.2004;5:803-13.
.Varanini Z,Pinton R.Direct versus indirect effects of soil humic substances on plant growth and nutrition. In: Pinton R, Varanini Z, Nannipieri P, eds. The Rizosphere. Basel: Marcel Dekker.2001:141-58.
.Hayat R,Ali S, Amara U, Khalid R, Ahmed I.Soil beneficial bacteria and their role in plant growth promotion, Ann Microbiol.2010;60:579–598.
.Galloway JN,Dentener FJ, Capone DG, Boyer EW, Howarth RW, Seitzinger SP.Nitrogen cycles: past, present, and future. Biogeochemistry.2004; 70,153–226.
.UmiKalsom MS,Nur H,Norlea AA and Ngaspan S.Characterization of humic acid from humification of oil palm empty fruit bunch fibre using Trichoderma viride, J. Trop. Agric. and Fd. Sc .2006;34(1): 165–172.
.Sardashti Alireza, Sangtarash Mohmamd Hossien,Asadi Amir.Defining the metal ions contaminants in the leaves and growing place soil of cultivated A.triphylla plant with addition of humic substances to root.IJACS.2015;8(2): 142-148.
.Sardashti Alireza, Gangali Amin,Kordi, Ali. Effect of humic substance on the qulity of essential oil of medicinal plants, Journal of medicinal plants Research.2012; Vol. 6(13): 2644-2645.
.Shekhghasemi AR, Shahryari R, Mollasadeghi V.Evaluation of different treatments of Humic on Pumpkin-naked seeds seeds in terms of the number of germination components. J. Farm. & Alli. Sci ,2013;2 (1): 18-22.
.Manske RHF,Holmes HL.The Alkaloids Chemistry and Physiology, Academic Press, INC., New York.1950: pp 1.
.Davies G, Ghabbour E,Steelink C.Humic Acids: Marvelous products of soil chemistry. J. Chem. Edu.2001;78(12): 1609-1613.
.Sardashti AR,Pourgharib Shahi T.Identification of Akaloids in Methanol Extract of Datura Stramonium Plant from Iran.American Journal of PharmTech
.Van Den, H., Kratz, PD.A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J. Chromatogr.1963;11:463-471.
.Adams RP.Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy. Allured Publ. Corp, Carol Stream, IL, USA. 1995.