HPLC-UV profile of Genista ulicina Spach. (Fabaceae) extracts and in vitro antioxidant activity

Authors

  • Kaoutar Chebbah Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et AnalysePhysico-chimiques et Biologiques (VARENBIOMOL), Université Frères Mentouri Constantine, Algérie.
  • Eric Marchioni Equipe de Chimie Analytique des Molécules Bioactives (IPHC-LC4, UMR 7178), Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin, 67400 Illkirch, France.
  • Djamel Sarri Département de Biologie, Université de M’sila, 28000 M’sila, Algérie.
  • Ahmed Menad Laboratoire de Biologie et Environnement, Université Frères Mentouri, Constantine. Route de Ain El Bey, 25 000 Constantine, Algérie.
  • Ratiba Mekkiou Department of Chemistry, Unit research: Valorisation des Ressources Naturelles et Analyses Physico-Chimiques et Biologiques, Université Constantine1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Ramdane Seghiri Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et AnalysePhysico-chimiques et Biologiques (VARENBIOMOL), Université Frères Mentouri Constantine, Algérie.
  • Ouahiba Boumaza Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et AnalysePhysico-chimiques et Biologiques (VARENBIOMOL), Université Frères Mentouri Constantine, Algérie.
  • Samir Benayache Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et AnalysePhysico-chimiques et Biologiques (VARENBIOMOL), Université Frères Mentouri Constantine, Algérie.
  • Fadila Benayache 1Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et AnalysePhysico-chimiques et Biologiques (VARENBIOMOL), Université Frères Mentouri Constantine, Algérie.

Keywords:

HPLC-DAD, Phenolic compounds, Antioxidant activity, Genista ulicina, Fabaceae

Abstract

To perform a qualitative and quantitative analysis of the phenolic and flavonoid contents and evaluate the antioxidant activity of ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts of the aerial parts of Genista ulicina Spach. from Algeria. The qualitative analysis of plant extracts was carried out by RP-HPLC using UV detector, whereas the quantification of total phenolic and flavonoid contents was completed according to the Folin-Ciocalteu procedure and aluminium chloride colorimetric method respectively. To evaluate the extract's antioxidant activity, Two in vitro antioxidant tests were employed: DPPH and β-carotene bleaching assay. The HPLC/DAD chromatogram showed several peaks indicating the presence of phenolic acids, flavonoids and isoflavonoids in both extracts. The total phenolic content (TPC) ranged from 62.56 and 50.45 mgGAE/g extract, while the total flavonoids content varied between 53.1 and 48.4 mgQE/g extract for EtOAC and n-BuOH respectively. EtOAc extract showed a maximum inhibition value (78.15%) at 150µg/mL using DPPH test and highest antioxidative power (82.42%) using β-carotene bleaching assay comparing with standards. The HPLC-UV analysis showed the richeness of both extracts in phenolic and flavonoid contents. The EtOAc extract exhibited good antioxidant activities comparing to the n-BuOH extract. Thus Genista ulicina could be indicated as a plant of phytopharmaceutical importance.

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Published

30-09-2016

How to Cite

1.
Kaoutar Chebbah, Eric Marchioni, Djamel Sarri, Ahmed Menad, Ratiba Mekkiou, Ramdane Seghiri, Ouahiba Boumaza, Samir Benayache, Fadila Benayache. HPLC-UV profile of Genista ulicina Spach. (Fabaceae) extracts and in vitro antioxidant activity. ijp [Internet]. 2016 Sep. 30 [cited 2024 Nov. 23];8(3):398-403. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/484

Issue

Vol. 8 No. 3 (2016): Jul- Sep

Section

Original Research Articles

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