In vitro antioxidant activities of n-butanol extract of Helianthemum confertum
Keywords:
Helianthemum confertum, antioxidants, DPPH, Lipid peroxidation, Free radical scavenging capacityAbstract
Antioxidants play an important role to protect damage caused by oxidative stress. Plants having phenolic contents are reported to possess antioxidant properties. The basic aim of this research was to investigate the antioxidant properties of n-butanol extract of Helianthemum confertum, Cistaceae family. The antioxidant activities and phenolic contents of n-butanol extract were evaluated in vitro using spectrophotometer methods. There antioxidant activities were determined by DPPH (1,1-diphenyl-2-picrylhydrazine) radical scavenging assay and lipid peroxidation inhibition by TBARS method. Antioxidant activities were compared to ascorbic acid. Measurement of total phenolic compounds and total flavonoids content of the n-butanol extract of Helianthemum confertum were achieved using Folin-Ciocalteau reagent and AlCl3 respectively. The results showed that this extract containing 263.33±19.85µg of gallic acid equivalents/mg extract of total phenolic and 25.35±3.15µg of quercetin equivalents/mg extract total flavonoids.
References
Mohd A, Ahsanullah K, Mohd M, Ajaz A, Sheeba U, Mohd A. Phytochemical Analysis and in vitro Antioxidant Activity of Zingiber officinale. Free Radicals and Antioxidants 2011; 1.
Deepa B, Prema G, Sai Krishna B, Cherian KM. Antioxidant and free radical scavenging activity of triphala determined by using different in vitro models. Journal of Medicinal Plant Research 2013 ; 7(39):2898-2905.
Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complementary and Alternative Medicine 2012; 12:221.
Amrani A, Boubekri N, Benaissa O, Zama D, Benayache F, Benayache Samir.In vitro antioxidant, antibacterial and membrane stabilizing activity of plant extract from chrysanthemum fontansii.International journal of phytomedcine 2013;5 :493-498.
Rahiman S, Tantry B.A, Kumar A.Variation of antioxidant activity and phenolic content of some common home remedies with storage time. Afr J Tradit Complement Altern Med 2013 ; 10(1):124-127.
Ashafa AOT, Grierson DS, Afolayan AJ. In vitro antioxidant activity of extracts from the leaves of felicia muricata thunb. An underutilized medicinal plant in the Eastern Cape province, South Africa. Afr J Tradit Complement Altern Med 2010 ; 7(4):296-302.
Khan MA, Abdur Rahman A, Shafiqul I, Khandokhar P, Parvin S,Md Badrul I, Mosharrof H, Mamunur R, Golam S, Shamima N,M Nurul Haque Mollah, AHM Khurshid Alam. A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L. (Moraceae). BMC Research Notes 2013;6:24.
Bushra A, Khan MR, Naseer AS, Rahmat AK. In vitro antioxidant potential of dicliptera roxburghiana. BMC Complementary and Alternative Medicine 2013; 13:140.
Quezel P. and Santa S. Nouvelle flore de l’Algérie et des régions désertiques méridionales. Editions du C.N.R.S, Paris. 1963, Tome II p. 714.
Singleton VL, Orthofer R, Lamuela- Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. In: Packer L, editor. Methods in enzymol: oxidant and antioxidants (part A), 299. San Diego, CA: Academic Pres 1999; 152-78.
Wang H, Dong Gao X, Zhou GC, Cai L, Yao WB.Food Chem 2008;106:888-895.
Bicha S, Amrani A, Benaissa O, León F, Zama D, Brouard I, Benayache S, Bentamene A, Benayache F. A flavonoid with high antioxidant effect from Centaurea acaulis L. Der Pharmacia Lettre 2013; 5 (6):24-30.
Braca A, Tommasi N De, Bari L Di, Pizza C, Politi M, Morelli I, J. Nat. Prod., 2001 ; 64 :892-895.
Cao Y, Ikeda I, Int. J. Biol. Macromol.2009; 45: 231-235.
Gul MZ, Farhan A, Anand KK, Qureshi IA, Ghazi IA.Antioxidant and antiproliferative activities of Abrus precatorius leaf extracts - an in vitro study. BMC Complementary and Alternative Medicine 2013; 13: 53.
Mehar Darukhshan K, Dipto B, Anindita B, Sharmila C. Oxidative DNA damage preventive activity and antioxidant potential of plants used in Unani system of medicine. BMC Complementary and Alternative Medicine 2010 ; 10: 77.
Boubekri N, Belloum Z, Boukaabache R, Amrani A, Kahoul N, Hamama W, Zama D, Boumaza O, Bouriche H, Benayache F, Benayache S. In vivo anti-inflammatory and in vitro antioxidant activities of Genista quadriflora Munby extracts. Der Pharmacia Lettre, 2014; 6(1):1-7.
