Effect of a Proprietary Commiphora mukul Gum Resin Extract and Medium-Chain Triglyceride Preparation (GU-MCT810) on hypoxia-inducible factor-1 pathway in HepG2 cell line


  • Cheppail Ramachandran Chief Science Officer and Sr. Research Scientist, Nicklaus Children's Hospital Miami Childrens Health System Miami, FL 33155, USA United States.
  • Gilda M. Portalatin Dharma Biomedical LLC, Miami, FL 33156 United States
  • Karl-W Quirin Flavex Naturextrakte, GmbH, Rehlingen, Germany.
  • Enrique Escalon Miami Children's Hospital, Miami, FL 33155 United States.
  • Steven J. Melnick Miami Children's Hospital, Miami, FL 33155 Dharma Biomedical LLC, Miami, FL 33156 United States.


Commiphora, hypoxia-inducible factor-1, HepG2 cell


The heterodimeric transcription factor HIF-1 is responsible for the regulation of genes that facilitate adaptation and survival of cells under hypoxic conditions. HIF-1 gene expression is also associated with angiogenesis, glucose transport, nitric oxide synthase activity and cell proliferation through the regulation of hundreds of genes associated with HIF-1 pathway. GU-MCT810 is a nutraceutical ingredient complex that includes a Commiphora mukul (guggul) extract prepared by a supercritical CO2-co-solvent extraction with ethanol and medium chain triglyceride (MCT) oil composed of C8 and C10 fatty acids. Since cancer cells use glycolytic pathway, 2-deoxyglucose (2-DG) has been reported to inhibit the glycolysis. We have investigated the anticancer potential of GU-MCT810 with and without 2-DG in HepG2 human hepatoma cell line. Even though GU-MCT810 and 2-DG are individually weakly cytotoxic, the combination is synergistic with combination index (CI) values of 0.21, 0.22 and 0.88 at IC50, IC75 and IC90 levels, respectively. The combination also showed a synergistic inhibitory effect on ATP-synthesis in HepG2 cells. GU-MCT810 inhibits CoCl2-induced HIF-1α expression significantly in a dose-dependent manner with complete inhibition at 50 µg/ml concentration. GU-MCT810 upregulates Bax and p21 genes and down regulates Bcl-2, BNIP3 and mutant p53 genes associated with apoptosis. It also down regulates proteins associated angiogenesis (VEGF, VEGF-R), cell proliferation (IGF-2), glucose transport (GLUT1) and adaptogenesis (HSP70 and HSP90). These results indicate that GU-MCT810 can be combined with 2-DG for inhibition of HIF-1 pathway genes which would be useful for elimination of refractory cancer cells present in the hypoxic region of human tumors.


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How to Cite

Cheppail Ramachandran, Gilda M. Portalatin, Karl-W Quirin, Enrique Escalon, Steven J. Melnick. Effect of a Proprietary Commiphora mukul Gum Resin Extract and Medium-Chain Triglyceride Preparation (GU-MCT810) on hypoxia-inducible factor-1 pathway in HepG2 cell line. ijp [Internet]. 2015 Sep. 30 [cited 2024 Apr. 24];7(3):324-36. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/421



Original Research Articles