Antioxidative and antidiabetic effects of naringin and curcumin in vitro and in vivo


  • Damir Sirovina Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, HR-10000 Zagreb, Croatia
  • Nada Oršolić Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, HR-10000 Zagreb, Croatia
  • Ivan Ivić University of Pécs, Medical School, Department of Pathophysiology and Gerontology, Pécs, Hungary Germany
  • Sanja Novak Department of Physiology and Immunology, Medical faculty Osijek, J.J. Strossmayer University Osijek, Osijek, Croatia.
  • Goran Gajski Mutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000 Zagreb, Croatia
  • Vera Garaj-Vrhovac Mutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000 Zagreb, Croatia
  • Marijana Zovko Končić Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, HR-10000 Zagreb, Croatia.


diabetes, naringin, curcumin, antioxidant activity, DNA damage


The aim of the present study was to assess whether naringin or curcumin can influence oxidative stress induced DNA damage in mice with alloxan-induced diabetes. Naringin or curcumin preparations (50 mg kg-1) were given intraperitoneally for 7 days. The antioxidant capacity of curcumin and naringin in vitro was evaluated using three assays which cover different aspects of antioxidant activity. In order to evaluate the effectiveness of naringin and curcumin in vivo we observed changes in body weight and survival of diabetic mice and used the comet and micronucleus assays. In vitro curcumin showed appreciable antioxidant properties, while naringin was much less effective. Naringin or curcumin administration to diabetic mice resulted in decreased DNA damage in lymphocytes and increased level of DNA damage in liver, kidney and reticulocytes. Administration of naringin and curcumin resulted in significant increase of the body weight and 100% survival of mice. Results suggests that antioxidant activity of naringin and curcumin leads to long time survival of diabetic mice and possible prevention of further oxidative damage, so they could be candidates for antidiabetic agent, but the precise targets of naringin and curcumin in diabetic mice are still to be clarified.

Author Biography

Nada Oršolić, Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, HR-10000 Zagreb, Croatia



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How to Cite

Damir Sirovina, Nada Oršolić, Ivan Ivić, Sanja Novak, Goran Gajski, Vera Garaj-Vrhovac, Marijana Zovko Končić. Antioxidative and antidiabetic effects of naringin and curcumin in vitro and in vivo. ijp [Internet]. 2015 Jun. 30 [cited 2024 Jun. 17];7(2):176-84. Available from:



Original Research Articles