Antifungal activity of Leptadenia reticulata Wight and Arn. aerial parts


  • M.K. Mishra Bhabha Pharmacy Research Institute, Bhopal, India-462026
  • P Tiwari. Bhabha Pharmacy Research Institute, Bhopal, India-462026
  • D.K Dash. Bhabha Pharmacy Research Institute, Bhopal, India-462026
  • Rajesh.S. Jadon Bansal College of Pharmacy, Bhopal, India.
  • G. Ghosh School of Pharmaceutical Sciences, SOA University, Bhubaneswar-751030
  • B.B. Barik University Department of Pharmaceutical Sciences, Utkal University,Bhubaneswar-751004


Leptadenia reticulata Wight and Arn., Antifungal activity, Minimum inhibitory concentration, Zone of inhibition, Clotrimazole


The petroleum ether, chloroform, acetone, methanol and aqueous extracts of the aerial parts of Leptadenia reticulata Wight and Arn. (Asclepiadaceae) were studied for in vitro antifungal activity against Aspergillus flavus, Aspergillus ruantti, Candida tropicalis, Candida albicans, Trichodermata viride and Trichodermata koningii respectively. The methanolic extract exhibited prominent antifungal activity against all the selected strains. Minimum inhibitory concentration of the extracts was performed by broth dilution method and the zone of inhibition was studied by agar disc diffusion method at concentrations of 2, 5 and 10mg/ml in DMSO. Cotrimazole (25μg/ml) was used as reference control for antifungal studies. Results of MIC study revealed the antifungal activities of the extracts against the tested strains in between concentration ranges 50-400μg/ml. The present study indicates the potential usefulness of L. reticulata aerial parts as antifungal agent.


WHO, The World Health Report (Changing

history, statistical annex., death by cause, sex

and mortality stratum in WHO regions,

estimates for 2002), Geneva, 2004, 120-121.

Dean DA and Burchard KW, Fungal infection

in surgical patients, Am. J. Surg., 1996, 171,


Gonzalez CE, Venzon D and Lee S, Clin.

Infect. Dis., Clinical trials for the study of

infectious diseases, 1996, 23, 515-521.

Cordell G A, Biodiversity and drug discoverya symbiotic relationship, Phytochemistry,

, 55, 463-480.

Sieradzki K, Wu SK and Tomasz A,

Inactivation of the Mithicillin resistance gene

mecA in vancomycin- resistance

staphylococcus aureus, Micro. Drug Resist.,

, 5, 253-257.

Martin KW and Ernst E, Herbal medicines for

treatment of fungal infections- a systematic

review of controlled clinical trials, Mycoses,

, 47(3-4), 87-92.

Kirtikar KR and Basu BD, In; Blatter E, Caius

JF and Mhaskar KS (Eds.) Indian Medicinal

Plants, International Book Distributors:

Dehradun, India, 1994, 1629-1630.

Anonymous. The Wealth of India-Raw

materials, NISCOM, CSIR, New Delhi, 1998,


Rastogi RP and Mehrotra BN, Compendium

of Indian Medicinal Plants, CDRI, Lucknow;

, 243-244.

Diallo C, Samake FB, Paulsen BS, Michaelsen

TE and Keita A, Wound healing plants in

Mali, the Bamaco region. An ethnobotanical

survey and complement fixation of water

extracts from selected plants, Pharm. Biol.,

, 40(2), 117-28.

Anjaria JV, Varia MR, Janakiraman K and

Gulati OD, Anti-oxidant potential of

Leptadenia reticulata Wight, Indian J. Exp.

Biol., 1975, 13(5), 448-449.

Sharma LN, Bose IJ and Rastogi AK,

Isolation of active metabolites, Indian J. Nat.

Prod., 2003, 19(3), 11-15.

Trease GE and Evans WC, Pharmacognosy,

ELBS Publication, New Delhi, 1985, 734.

Tyler VE, Brady LR and Robbers JE,

Pharmacognosy, Lea and Febiger Publication,

Philadelphia, 1985, 21.

Cruickshank R, The Practice of Medical

Microbiology, Churchill Livingstone, London,

, 98.

Karwa VG, Sathawane PN, Kasture VS,

Kasture SB and Pal SC, Evaluation of anti

fungal activity by agar disc diffusion method,

Indian Drugs, 1997, 34(3), 174-176.

Hirano R, Sasamoto W, Matsumoto A,

Antioxidant ability of various flavonoids

against DPPH radicals LDL oxidation, J. Nutr.

Sci. Vitaminol., 2001, 47, 357.

Sengul M, Ogutcu H and Adiguzel A, Anti

microbial effects of Verbascum georgicum

Bentham extract, Turk. J Biol., 2005, 29, 105-

Ozturk S and Ercisli S, The chemical

composition of essential oil and in-vitro

antibacterial activities of essential oil and

methanol extract of Ziziphora persica Bunge,

J Ethnopharmacol., 2006, 106, 372-376.

Barnabas CG and Nagarajan S, Role of

flavonoids and tannins in some medicinal

plants: a review, Fitoterapia, 1988, 3,508-510.

Burapedjo S and Bunchoo A, Antimicrobial

activity of tannins from Terminalia citrina,

Planta Medica, 1995, 61, 365-366.




How to Cite

M.K. Mishra, P Tiwari., D.K Dash., Rajesh.S. Jadon, G. Ghosh, B.B. Barik. Antifungal activity of Leptadenia reticulata Wight and Arn. aerial parts. ijp [Internet]. 2010 Jun. 30 [cited 2024 Jun. 21];2(2):172-6. Available from:



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