Determination of cellular protection and leukemic cell inhibition by herbal extracts in cow urine.
Keywords:
AML-Acute myeloid leukemia ALL-Acute lymphoblastic leukemia CML-chronic myelogenous leukemiaAbstract
Cow urine is used as a therapeutic agent and a most valuable secretion of animal origin and herbs have been used as a antioxidant and chemotherapeutic agents,therefore we evaluated the cell protective and cell inhibitory activity of different herbs/spices which was prepared in cow urine( in order to enhance the activity) by MTT assay, and performed the above assay in blood cancer cells viz.ALL, AML, CML type of cancers.The results suggest that revealed that the drugs tested Ajwain, garlic, Dhania, Satavar, and Guduchi prepared in cow urine showed significant antioxidant activity or cell proliferative activity whereas, the extracts such as Dalchini, evaporated cow urine showed an excellent cell inhibition activity in all the three types of cancers viz. ALL, AML and CML. Kalmegha is showing cell inhibitory property for ALL cells but not for AML and CML cells, similarly Garlic showed cell inhibition property for AML but not for ALL and CML cells.
References
. Tulassay Z. Herszenyi L.,‰Gastric
mucosal defense and
cytoprotection.‰Best pract Res Clin
Gastroenterol. 2010;24 (2):99-108.
. Wallace J L.,ÊÊProstaglandins,NSAIDs
and cytoprotection.‰ Gastroenterol. Clin
North America,1992; 21 (3):631-41.
. Seirifried H E and Anderson D
E.,Areview of the interaction among
dietry antioxidants and reactive oxygen
species.,Journal of Nutritional
Biochemistry,2007.
. Bernas T, and Dobrucki
J.,‰Mitochondrial and non-mitochondrial
reduction of MTT:Interaction of MTT
with TMRE,JC-1 and NAO
mitochondrial fluorescent
probes,2010;47 (4):236-242.
. Kumar A, Kumar P, Singh L K and
Agrawal DK. Pathogenic effects of free
radicals and their prevention through
cowpathy. The Indian Cow,2004;6:27-
. Prabhar K,Structural dynamics of
apoptosis in avian
lymphocytes.M.V.Sc.thesis submitted
to college of veternery sciences.GB
plant University of agriculture and
technology ,Panta nagar,India.
. Krishnamurthy K, Dutta D and
Chakrabarti T. Protective effects of
distillate and redistillate of cowÊs urine
in human polymorphonuclear
leukocytes challenged with established
genotoxic chemicals.,Biomed Environ
Sci,2004;3 (6):123-126.
. Vijayalakshmi R and Sarayna V T
K.,‰Effects of Go-Mutra on plant growth
and its Antifungal and Antimicrobial
activity, International Journal of
Pharmaceutical Sciences and
research, 2010;6:1-11.
. Kong Y C and Chen D S,Elucidation of
Islamic drugs in hui hui yao fang:a
linguistic and pharmaceutical
approach, journal of
ethnopharmacology,1996;54:85-102.
. Krishnamurthy K, Dutta D and
Chakrabarti T. Protective effects of
distillate and redistillate of cowÊs urine
in human polymorphonuclear
leukocytes challenged with established
genotoxic chemicals.,Biomed Environ
Sci,2004;3 (6):123-126.
. Pathak Ak, Agrawal A, Jain DC,
Howarth OW :NMR studies of 20
hydroxyedysome,a steroid isolated
from T.cardifolia:Indian journal of
chemistry.,1995;34:674-678.
