Preliminary phytochemical screening and antimicrobial activity of fresh plant extract of Indian folk medicinal plant, Gnaphalium polycaulon
Keywords:
Phytochemicals, phenols, flavonoids, steroids, alkaloids, antimicrobial activityAbstract
Nature has been a source of medicinal agents for thousands of years. Plants produce a diverse array of bioactive molecules, making them a rich source of diverse type of medicines. Thus, natural products with pharmacological or biological activities still play a very important role in medicine. The objective of the present study deals with the exploration of specific species of medicinal plants found in Western Ghats, India and was to investigate the presence of phytochemicals constituents and to determine antimicrobial analysis of the Gnaphalium polycaulon in hexane solvents. Fresh G. polycaulon was air dried and coarsely powdered. Then all the extracts obtained were subjected for phytochemical screening using standard procedure. Bacterial and fungal cultures were used for screening antimicrobial study by agar well diffusion method. It reported the presence of major phytochemicals in all solvent extracts. The phytochemical of the plant extract showed that the leaf and stem were rich in major phytochemicals such as alkaloids, flavonoids, tannins and saponins, than flower extract. The resource base of the traditional folk medical practices is more prevalent in rural and tribal villages of India. Our findings provided evidence that crude hexane extracts of selected plants contain medicinally important bioactive compounds and their use in the traditional folk medicines practices.
References
Hemraj Vashist, Anil Jindal. Antimicrobial Activities of Medicinal Plants–Review. Int J Res Pharm Biomed Sci. 2012; 3 (1):222-230.
Bhore NV, Pishawikar SA, More, HN. Phytochemical Screening and Antioxidant Activity of Flowers (inflorescence) of Saccharum Officinarum Linn. Int J Res Pharm Biomed Sci. 2012; 3 (2): 620-624.
Subashkumar R, Sureshkumar M, Babu S, Thayumanavan Tha. Antibacterial effect of crude aqueous extract of Piper beetle L. against pathogenic bacteria. Int J Res Pharm Biomed Sci. 2013;4(1):42-46.
Shanmugapriya K, Thayumanavan Tha. Phytochemical and antimicrobial activities of Gnaphalium polycaulon. In: Scientific Basis of Herbal Medicine; (ed.): Parimelazhagan Thangaraj. Bharathiar University, Coimbatore (pp 47-59). Abstract presented at the National Conference on Phytomedicine, at Bharathiar University, Coimbatore on 04-05, October 2012. ISBN: 9788170358732: 47-59, (2013).
Shyma TB, Deepa Shree CL, Devi Prasad AG, Shubha Gopal, Komal Kumar J. Preliminary phytochemical screening and bioactivity of selected Indian medicinal plants. Int J phytomed.2013; 5(1):1-6.
Bharti D,Gupta S, Arora chugh C. Antimicrobial activity of medicinal plants against some pathogenic microbial skin. Int J phytomed. 2013; 5(2):154-158.
Thenmozhi S, Rajan S. Screening of antibacterial and phytochemical activity of Acalypha indica Linn against isolated respiratory pathogens. Res Plant Biology. 2012; 2(1): 01-06.
Ramesh Londonker, Asha Tukappa NK. pharmacognosticsal evaluation and comparative phytochemical screening of Rumex vesicarius L. Int J phytomed. 2013; 5(2):146-153.
Lakshman Raju Badugu. Phytochemical Screening, Quantitative Estimation Total Phenolics and Total Flavonoids, Anti Microbial Evaluation of Cyamopsis tetragonoloba. Int J Res Pharm Biomedical Sci. 2012; 3 (3):1139-1142.
Parekh J, Karathia N, Chanda S. Evaluation of antibacterial activity and phytochemical analysis of Bauhinia variegata L. bark. Afr J Biomed Res. 2006; 9: 53-56.
Shanmugapriya K, Saravana PS, Harsha Payal, Peer Mohammed, Williams Binnie. A comparative study of antimicrobial potential and phytochemical analysis of Artocarpus heterophyllus and Manilkara zapota seed extracts. J Pharm Res. 2011; 4(8): 2587-2589.
Rehana kouser, Rahmatullah Qureshi. Antimicrobial activity of Fagonia indica from Thai desert, Punjab, Pakistan. Int J phytomed. 2013; 5(1):113-118.
Ahmad J, Mehmood Z, Mohammad F. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol. 1998; 62: 183-193.
Joseph M, Charles A, Alex Ramani V. phytochemical studies of Phyllanthus wightianus leaf extract. Int J phytomed.2012; 4(4); 456-460.
Bhupendra S Adhikari, Mani M Babu. Floral diversity of Baanganga Wetland, Uttarakhand, India. Check List .2008; 4(3): 279-290.
Sandeep acharya. Presage biology: lesson from nature in weather forecasting. Ind J trad knowl. 2011; 10(1): 114-124.
Marjorie C. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999; 12: 564-582.
Odebiyi EO, Ramstard AH. Investigation photochemical screening and antimicrobial screening of extracts of Tetracarpidium conophorum. J Agric Chem Soc. 1978; 26: 1-7.
Harborne, 1998 Phytochemical Methods: A guide to Modern Techniques of Plant Analysis, Chapman and Hall, London, UK, 2nd edition.
Buvaneswari K, Ramamoorthy D, Velanganni J. Preliminary Phytochemical and Antimicrobial Activity Studies on the Leaves of the Indian Plant Thevetia neriifolia Juss. World J Agric Sci. 2011; 7(6):659-666.
Sibaram Paria, Subhasish Maity, Musfiqua Mookerjee Phytochemial Investigation and Evaluation of Anthelmintic activities of V.Negundo Leaf Extract. Int J Res Pharm Biomed Sci. 2012; 3 (3): 1143-1146.
Sardashti AR, Ganjali A. Chemical composition and antimicrobial activity of essential oil from Artemisia lehmanniana. Int J of phytomed. 2013; 5(2): 85-88.
Yadav RNS, Munin Agarwala. Phytochemical analysis of some medicinal plants. J Phytol. 2011; 3(12): 10-14.
Shanmugapriya K, Saravana PS, Harsha Payal, Peer Mohammed, Williams Binnie. Antioxidant potential of pepper (Piper nigrum Linn.) leaves and its antimicrobial potential against some pathogenic microbes. Ind J Nat Prod Res. 2012; 3 (4): 570-577.
Aguinaldo AM, El-Espeso, Guovara BQ, Nanoto MG. Phytochemistry. In: Guevara B.Q (ed.) A Guide book to plant screening phytochemical and biological. University of Santo Tomas, Manila, Philippines. 2005.
Mandal P, Sinha Babu SP, Mandal NC. Antimicrobial activity of Saponins from Acacia auriculiformis. Fitoterapia. 2005; 76(5):462-565.
Okwu DE. Phytochemical and Vitamin content of indigenous species of South Eastern Nigeria. J Sustain Agric Environ. 2004; 6:30-34.
Verma RK, Chaurasia L,Kumar M. Antifungal activity of essential oils against selected building fungi. Ind J Nat Prod Res. 2011; 2(4): 448-451.
Arora Chugh C, Bharti D. Phytochemical analysis and screening of antibacterial activity of some selected Indian medicinal plants. Int J phytomed.2012; 4(2): 261-265.
