Evaluation of anti-inflammatory activity of ethanolic whole plant extract of Desmodium gangeticum L.
Keywords:
Desmodiumgangeticum, anti-inflammatory activity, Carrageenan, Diclofenac SodiumAbstract
The present study was designed to investigate the anti-inflammatory activity of the ethanolic extract of whole plant of Desmodium gangeticum. Investigations were done by Carrageenan-induced paw oedema method in rats. Here the extract at the 100 & 200mg/kg dose level showed 36.68% (p<0.001) inhibition of edema volume at the end of 4hr .The extract reduced significantly the formation of oedema induced by carrageenan. Inflammatory diseases including different types of rheumatic diseases are very common throughout the world. Therefore the search for a better tolerated anti-inflammatory agent appears to be a necessity. Desmodium gangeticum is used as a folk medicine for the treatment of inflammation in India. Present study revealed that the plant Desmodium gangeticum possesses a significant anti-inflammatory activity as evidenced in carrageenan induced paw edema method, which supports the folkloric claim of the anti-inflammatory activity of the plant.
References
. Bohlin L. Structure-activity studies of
natural products with anti-inflammatory
effects. In: Hostettmann, K. (Ed.),
Phytochemistry of Plants used in
Traditional Medicine. Clarendon Press,
Oxford, pp. (1995). 137-161.
. Yesilada E, Ustun O, Sezik E, Takishi
Y, Ono Y. Honda G. Inhibitory effects of
Turkish folk remedies on inflammatory
cytokines: interleukin-1đ, interleukin- 1ß
and tumor necrosis factor-đ. J
Ethnopharmacol (1997). 58, 59-73.
. Li RW, Myers SP, Leach DN, Lin G D,
Leach G. A cross-cultural study: antiinflammatory activity of Australian and
Chinese plants. J Ethnopharmacol
(2003). 85, 25-32.
. Rocha e Silva M. A brief survey of the
history of inflammation. 1978. Agents
Actions1994;43:86ă90
. Sujit K.Chaudhary Quitessence Of
Medical Pharmacology,New
CentralBook Agency (P) Ltd Kolkata,
(2001) 400.
. Denko CW. A role of neuropeptides in
inflammation. In: Whicher JT,
EvansSW, editors. Biochemistry
ofInflammation, London: Kluwer Pub,
(1992) 177-181.
. Henson PM, Murphy RC. Mediators of
the inflammatory process.6th ed.
Amsterdam: Elsevier(1989).
. Gambhire MN, Wankhede SS, Juvekar
AR. Journal of Young Pharmacists,
(2009) 1, 3; 220-224.
. Ogunyemi AO. The origin of herbal cure
and its spread; proceedings of a
Conference on African Medicinal
Plants. Sofowora A. (Ed.) University
Press, Ile-Ife, (1979) 20-22.
. Chattopadhyay RR, RN, Banerjee, SK,
Sarkar S, Ganguly TK. Basu, Antiinflammatory and acute toxicity studies
with leaves of Vinca rosea Linn in
experimental animals. Indian J. Physiol.
Pharmacol. 1992, 36: 291-292.
. Chopra RN, Nayar SL, Chopra I C.
„Glossary of Indian Medicinal Plants,‰
Council of Scientific and Industrial
Research, New Delhi, 1956, p. 94.
. Ghosh D, Anandkumar A.
Antiinflammatory and analgesic
activities of gangetinăapterocarpenoid
from Desmodium gangeticum, Indian J.
Pharmacol. 15 (1981) 391ă402
. Purushothman KK, Kishore VM,
Narayanaswamy V. The structure and
stereochemistry of gangetin. A new
pterocarpan from Desmodium
gangeticum, J. Chem. Soc. 1971,
ă2422.
. OECD/ OCD. 425 OECD Guidelines for
testing of chemicals acute oral toxicity
Up And Down procedure, (2001) 26; 1-
. Turner RA. Screening method in
pharmacolog New York: Academic
Press, 152. (1965)
. Winter EA, Risley EA, Nuss GW.
Journal of Pharmacology and
Experimental Therapeutics, (1963)
; 369-373.
. Garcialeme J, Nakamura L, Leite MP,
Rochae SM. Pharmacological analysis
of the acute inflammatory process
induced in ratÊs paw by local injection of
carrgeenan and by heating. British J.
Pharmacol. 1973. 48, 88-96.
. Vane J, Booting R. Inflammation and
mechanism of action of antiinflammatory drugs FASEB J, 1987. 1:
-96.
. Vinegar R, Schriber W, Hugo R. Journal
of Pharmacology and Experimental
Therapeutics, (1969) 166; 96-103.
. Alcatraz MJ, Jimenez MJ. Flavonoids
as anti-inflammatory agents.
Fitoterapia, 1998. 59: 25-38.
. Ozaki Y. Anti-inflammatory effect of
Curcuma xanthorrhiza Roxb, and its
active principles. Chem. Pharm. Bull.
(Tokyo), (1990). 38(4): 1045-1048.
. Silva GN, Martins FR, Matheus ME,
Leitão SG, Fernandes PD. Investigation
of anti- inflammatory and
antinociceptive activities of Lantana
trifolia. J. Ethnopharmacol. (2005).
(3): 254-259.
. Perianayagam, JB, Sharma SK, Pillai
KK. Antiinflammatory activity
of Trichodesma indicum root extract in
experimental animals. J.
Ethnopharmacol. 2006.104: 410-414.
. Mossai JS, Rafatullah S, Gala AM, AlYahya MA. Pharmacological studies of
Rhus retinorrhaea. Int. J. Pharmacog.
33, 242-246.
. Sawadogo WR, Boly R, Lompo M,
Some N. Anti-inflammatory, analgesic
and antipyretic activities of Dicliptera
verticillata. Int. J. Pharmacol. 2006.2,
-273.
. Bohlin L. Structure-activity studies of
natural products with anti- inflammatory
effects, 1995.137-161. In: Hostettmann,
K. (Ed.). Photochemistry of plants used
in traditional medicine. Clarendon.
Oxford, UK.
. Brooks PM, Hill W, Geddes R.
Diclofenac and ibuprofen in rheumatoid
arthritis and osteoarthritis. Med. J. Aust,
(1980). 1: 29-30.
