Anti-Helicobacter pylori, proton pump inhibitory and antioxidant properties of selected dietary/medicinal plants
Keywords:
Helicobacter pylori, H , K -ATPase, Antioxidant, Gastric ulcer, Antiulcer indexAbstract
In recent days, emphasis has been on the use of edible sources for maintenance of health. H. pylori being a main causative organism of gastric cancer and ulcers, many researchers are working in this area to overcome the problem. The plants selected for the current study have application in traditional medicine for the treatment of gastrointestinal diseases. The studies provide the scientific evidence to antiulcer potential of medicinal/dietary plants of ethnopharmacological importance and categorize them based on multi-potent antiulcer activity. In the current study anti-Helicobacter pylori, H+, K+ -ATPase inhibitory and antioxidant activity of aqueous extracts of 18 different plants were investigated. The overall antiulcer potency of selected plants was calculated as antiulcer index (AUI) based on these in vitro antiulcer activities and categorized the plants into 4 groups based on their AUI. Overall study revealed Decalepis hamiltonii as very good (AUI 81) followed by Nigella sativa, Coscinium fenestratum and Zingiber officinale as good source (AUI 61-68) for antiulcer therapy. Among the rest, 9 plants were categorized as moderate with AUI of 41 to 59 and 6 plants as poor sources with AUI of 20 to 37. Thus the results of the current study contribute towards validation of the traditional use of selected plant sources against gastrointestinal disorders.
References
. Lam SK. Differences in peptic ulcer between East and West. Baillieres Best Pract Res Clin Gastroenterol. 2000;14:41-52.
. Das D, Banerjee RK. Effect of stress on the antioxidant enzymes and gastric ulceration. Mol Cell Biochem.1993;125:115-125.
. Okabe S, Amagase K. An overview of acetic acid ulcer models – the history and state of the art of peptic ulcer research. Biol Pharm Bull. 2005;28:1321-1341.
. Tarnawski AS. Cellular and molecular mechanisms of gastrointestinal ulcer healing. Dig Dis Sci. 2005;50:S24–S33.
. Demir S, Yilmaz M, Köseoğlu M, Akalin N, Aslan D, Aydin A. Role of free radicals in peptic ulcer and gastritis. Turk J Gastroenterol. 2003;14:39-43.
. Phull PS, Green CJ, Jacyna MR. A radical view of the stomach: the role of oxygen-derived free radicals and anti-oxidants in gastroduodenal disease. Eur J Gastroenterol Hepatol. 1995;7:265-274.
. Srikanta BM, Siddaraju MN, Dharmesh SM. A novel phenol-bound pectic polysaccharide from Decalepis hamiltonii with multi-step ulcer preventive activity. World J Gastroenterol. 2007;13:5196-5207.
. Srikanta BM, Sathisha UV, Dharmesh SM. Alterations of matrix metalloproteinases, gastric mucin and prostaglandin E2 levels by pectic polysaccharide of swallow root (Decalepis hamiltonii) during ulcer healing. Biochimie. 2010;92:194-203.
. Schraufstatter I, Hyslop PA, Jackson JH, Cochrane CG. Oxidant induced DNA damage of target cells. The Journal of Clinical Investigation. 1988;82:1040-1050.
. Sachs G, Chang HH, Rabon E, Schackman R, Lewin M, Saccomani G. A non electrogenic H+ pump in plasma membranes of hog stomach. J Biol Chem. 1976;251:7960-7968.
. Salas M, Ward A, Caro J. Are proton pump inhibitors the first choice for acute treatment of gastric ulcers? A meta analysis of randomized clinical trials. BMC Gastroenterol. 2002;2:17-23.
. Vestergaard P, Rejnmark L, Mosekilde L. Proton pump inhibitors, histamine H2 receptor antagonists, and other antacid medications and the risk of fracture. Calcif Tissue Int. 2006;79:76-83.
. Marshall BJ, Warren JR. Unidentified curved bacillus on gastric epithelium in active chronic gastritis. Lancet. 1983;1:1273-1275.
. Hansson LE, Engstrand L, Nyren O, Evans DJ, Lindgren A, Bergstrom R, Anderson B, Athlin L, Bendston O, Tracz P. Helicobacter pylori infection: independent risk factor of gastric adenocarcinoma. Gastroenterol. 1993;105:1091-103.
. Parsonnet J, Hansen S, Rodriguez L, Gelb AB, Warnke RA, Jellum E, Orentreich N, Vogelman JH, Freidman GD. Helicobacter pylori infection and gastric lymphoma. N Engl J Med. 1994;330:1267-1271.
. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12:564–582.
. Reyes-Chilpa R, Baggio CH, Alavez-Solano D, Estrada-Mu˜niz E, Kauffman FC, Sanchez RI, Mesia-Vela S. Inhibition of gastric H+,K+-ATPase activity by flavonoids, coumarins and xanthones isolated from Mexican medicinal plants. J Ethnopharmacol. 2006;105:167–172.
. Friedman M, Henika PR, Mandrell RE. Antibacterial Activities of Phenolic Benzaldehydes and Benzoic Acids against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enteric. J Food Prot. 2003;66: 1811-1821.
. Friedman M. Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas. Mol Nutr Food Res. 2007;51:116–134.
. Traci LT, David JMG, Harry LTM. Helicobacter pylori growth and urease detection in the chemically defined medium Ham’s F-12 nutrient mixture. J Clin Microbiol. 2001;39:3842–3850.
. Siddaraju MN, Dharmesh SM. Inhibition of gastric H+, K+-ATPase and Helicobacter pylori growth by phenolic antioxidants of Zingiber officinale. Mol Nutr Food Res. 2007;51:324–332.
. Srikanta BM, Harish Nayaka MA, Dharmesh SM. Inhibition of Helicobacter pylori growth and its cytotoxicity by 2-hydroxy 4-methoxy benzaldehyde of Decalepis hamiltonii (Wight & Arn); a new functional attribute. Biochimie. 2011;93:678-688.
. Gamble JS, Fisher CEC. Flora of the presidency of Madras, Reprinted Edition, BSI, Culcutta, India, 1957;Vol. I-III.
. Singleton VL, Rossi JAJr. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic reagents. Am J Enol Vitic. 1965;16:144–158.
. Braca A, Fico G, Morelli I, De Simone F, Tomè F, De Tommasi N. Antioxidant and free radical scavenging activity of flavonol glycosides from different Aconitum species. J Ethnopharmacol. 2003;86:63-67.
. Yen GC, Chen HY. Antioxidant activity of various tea extracts in relation to their antimutagenecity. J Agric Food Chem. 1995;43:27-32.
. O’Mahony R, Al-Khtheeri H, Weerasekera D, Fernando N, Vaira D, Holton J, Basset C. Bactericidal and anti-adhesive properties of culinary and medicinal plants against Helicobacter pylori. World J Gastroenterol. 2005;11:7499-7507.
. Junko K, Masaru S, Kazuo T. A new method for preparing electron microscopic specimens of Helicobacter pylori. Med Electron Microsc. 1999;32:, 62-65.
. Puttaraju NG, Venkateshaiah SU, Dharmesh SM, Nanjaraj Urs SM, Somasundaram R. Antioxidant activity of indigenous edible mushrooms. J Agric Food Chem. 2006;54:9764–9772.
. Naik Y, Jayaram S, Harish Nayaka MA, Lakshman, Dharmesh SM. Gastroprotective effect of swallow root (Decalepis hamiltonii) extract: possible involvement of H(+)-K(+)-ATPase inhibition and antioxidative mechanism. J Ethnopharmacol. 2007;112:173-179.
. Siddaraju MN, Dharmesh SM. Inhibition of gastric H+, K+-ATPase and Helicobacter pylori growth by phenolic antioxidants of Curcuma amada. J Agric Food Chem. 2007;55:7377-7386.
. Cipriani TR, Mellinger CG, de Souza LM, Baggio CH, Freitas CS, Marques MC, Gorin PA, Sassaki GL, Iacomini M. Polysaccharide from a tea (infusion) of Maytenus ilicifolia leaves with anti-ulcer protective effects. J Nat Prod. 2006;69: 1018-1021.
. Nergard CS, Kiyohara H, Reynolds JC, Oates JET, Matsumoto T, Yamada H, Patel T, Petersen D, Michaelsen TE, Diallo D, Paulsen BS. Structures and structure-activity relationships of three mitogenic and complement fixing pectic arabinogalactans from the malian antiulcer plants Cochlospermum tinctorium A.Rich and Vernonia kotschyana Sch. Bip. ex Walp. Biomacromoles. 2006;7:71-79.
. Yang M, Sun H, Yu DW, Cui ZY, Tian J. Effects of the polysaccharide isolated from Ganoderma applanatum (PGA) on the level of PGE2 and gastric mucosal blood flow (GMBF) and gastric mucus secretion of rats with gastric mucosa injury. Zhongguo Zhong Yao Za Zhi. 2005;30:1176-1178.
