Antidiabetic and antihyperlipidemic effects of alcoholic and aqueous stem bark extracts of Limonia acidissima, linn in alloxan induced diabetic rats

Authors

  • Bhupendra Kumar Kumawat Punjab College of Pharmacy, Bathinda-151001, Punjab, India
  • Tara Chand Regional College of Pharmacy, Jaipur-302022, Rajasthan, India.
  • Yogendra Singh Shanti Niketan College of Pharmacy, Mandi-175008, Himachal Pradesh, India.

Keywords:

Limonia acidissima Linn, Antidiabetic activity, Antihyperlipidemic activity, Alloxan, Histopathology

Abstract

The present study was designed to investigate the antidiabetic and antihyperlipidemic effects of the alcoholic and aqueous extract obtained from the stem bark of Limonia acidissimaLinn (Rutaceae) using alloxan induced diabetic model in rats for 14 days. The alcoholic extract (200mg/kg) showed significant activity (P<0.01) in lowering blood glucose level than the aqueous extract (200mg/kg) which was comparable to glibenclamide (5mg/kg), a standard antidiabetic drug. Different biochemical parameters i.e. TGL(triglycerides), HDL, LDL, VLDL and total cholesterol level were also carried out which show that both alcoholic and aqueous extracts has significantly (p<0.001) reversed the diabetes-included hyperlipidemia compared to standard drug. Concurrent histological studies of the pancreas of these animals showed comparable regeneration by alcoholic and aqueous extracts which were earlier, necrosed by alloxan. The data suggests that the alcoholic extracts (200 mg/kg) may be effectively utilized as antidiabetic and antihyperlipidemic agents and supports its traditional usage in the control of diabetes. However, further pharmacological evaluations are required to isolate and identify the active antidiabetic and antihyperlipidemic principles in the plant as well as elucidating their mechanisms of action.

References

. The wealth of India, Institute of

science communication; Council of

Scientific & Industrial Research, New

Delhi, India, Ist edition, 1956;Reprint

:Vol-IV:18-20.

. Vaidaya ratnam P.S. Varier, Arya

Vaidya sala, Kottakkal, Indian

medicinal plants, Orient Longman

Ltd., Madras, Vol-III, 1995:327-332.

. Chopra RN, Nayar SL, Chopra IC.,

Glossary of Indian medicinal plants,

PID, New Delhi, 1956:117.

. Gupta VC, Hussain SJ, Imam S,

Important folk-medicinal plants and

traditional knowledge of tribals of

Aurangabad and Nasik forest divisions

of Maharashtra, India. Hamdard

Medicus, 1997;40:59-61.

. Morton J, Wood-Apple. In: Fruits of

warm climates. Julia F. Morton, Miami,

FL, 1987;190-191.

. FRLHT’s Amruth, jan.-fab. Vol.-8,

Issue-1,2004;14.

. Smith Albert C, Flora Vitiensis nova: a

new flora of Fiji, National Tropical

Botanical Garden, Lawai, Kauai,

Hawaii, 1985;3:526,527,758.

. Kirtikar KR, Basu BD, Indian medicinal

plants, Vol.-I, Published by

International book distributors,

Dehradun, 2005;496-499.

. Dr. Duke’s phytochemical and

ethanobotenical Databases.

. Macleod JK, Moeller DR, Bandara

BMR, Gunatilaka AAL, Wijeratne

EMK, Acidissimin: a new limonoid

from Limonia acidissima, Journal of

natural products, 1989;52(4):882-885.

. Kumawat BK, Rajkumar M, singh Y,

Antidiarrhoeal activity of alcoholic and

aqueous extracts of Limonia

acidissima, Linn. stem bark, Indian J.

Nat. Prod, 2010;26(4):23-27.

. Harborne JB. Phytochemical methods,

A guide to modern techniques of plant

analysis, III edition, 2005;4-8,72-

,134-135.

. Furis BS, Hannaford AJ, Rajesh V,

Suilap NG and Taichan AR. Vogels

Text Book of Practical Organic

Chemistry, Longman group

publication, 1978;400-402.

. Donald J. Ecobiscon, The basis of

toxicity testing, 2nd Edition. CRC

Press, 1997;43-49.

. Organization for Economic Cooperation and Development,

Guidance document on Acute Oral

Toxocity Testing, Environment

Directorate, OECD, Peris, 2001;1-24.

. Ragava NB. and krishnakumari S,

antidiabetic effect of t. arjuna bark

extract in alloxan induced diabetic

rats, Indian Journal of Clinical

Biochemistry, 2006;21(2):123-128.

. Deore SL, Khadabadi SS., Daulatkar

VD, Deokate UA, Farooqui IU.

Evaluation of hypoglycemic and

antidiabetic activity of bark of Butea

monosperma, Phcog Mag.,

;4(Suppl 13):16,18

. Staley P, Kammakakannan N,

Venugopal P. Menon. Anti diabetic

and hyper lipedemic effect of alcoholic

Syzigium cumini seeds in alloxan

induced diabetic albino rats, Journal of

Ethano pharmacology, 2004;91:209-

. Triender P, Determination of glucose

using glucose oxidase with an

alternative oxygen acceptor, Annual

Clinical Biochemistry,1969;6:24–27.

. Vogel H. Gerhard, Drug discovery and

Evaluation pharmacological Assays,

Springer-Verlag Berlin Heidelberg,

New York, 2002;947-1051.

. Snedeeor GW, Cochran WG, in:

Statistical Methods, Oxford and IBII

Publishing Co., New Delhi, 1967;33.

. Lillie RD. Histopathologic Technic and

Practical Histochemistry, McGraw-Hill

Company, Toronto, 1954;114.

. Nagappa AN, Thakurdesai PA, Venkat

Rao N, Singh J. Antidiabetic activity of

Terminalia catappa Linn fruits, J.

Ethnopharmacol, 2003;88:45-50.

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Published

30-06-2012

How to Cite

1.
Bhupendra Kumar Kumawat, Tara Chand, Yogendra Singh. Antidiabetic and antihyperlipidemic effects of alcoholic and aqueous stem bark extracts of Limonia acidissima, linn in alloxan induced diabetic rats. ijp [Internet]. 2012 Jun. 30 [cited 2024 Dec. 6];4(2):187-96. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/162

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