Hepatoprotective activity of aqueous extract of Balanites aegyptiaca L. Delile (Balanitaceae) roots bark
Keywords:Balanites aegyptiaca, Aqueous extract, Acute toxicity, Hepatotoxicity, Carbon tetrachloride
Balanites aegyptiaca (L.) Del (Balanitaceae) is traditionally used for the treatment of various ailments such as syphilis, jaundice and liver disorders, epilepsy, ... This study was designed to evaluate acute toxicity and hepatoprotective effect of aqueous extract of Balanites aegyptiaca on CCl4 induced hepatotoxicity in rats. Methods: Acute toxicity was assessed with the extract at a dose of 2000 mg / kg bw. The extract at doses of 25, 50 and 100 mg / kg b.w. was orally administered respectively to CC14-induced hepatotoxicity (0.5 ml / kg) animals. Silymarin (100 mg / kg) was given as a reference. Biochemical parameters such as ALT, AST, PT, ALB and ALP were assayed as well as enzymatic antioxidant activities SOD, CAT and MDA. Nitrogen monoxide (NO) involved in inflammation was also measured. Results: Activities of liver marker enzymes, ALT, AST and ALP, total protein, albumin and showed a significant hepatoprotective effect. Regarding antioxidant enzymatic activities in vivo (SOD, CAT and MDA) of aqueous extract exhibited a significant effect showing increasing levels of SOD, CAT and reducing malondialdehyde (MDA) levels. The production of NO is significantly reduced compared to the batch intoxicated by CCl4. Conclusion: Balanites aegyptiaca is endowed with hepatoprotective properties that can be attributed to antioxidant potential which could justify its use in traditional medicine in liver disorders.
Srivastava R, Srivastava P. Hepatotoxicity and the Role of Some Herbal Hepatoprotective Plants in Present Scenario 2018. 2. Elberry AA, Harraz FM, Ghareib SA, Nagy AA, Gabr SA, Suliaman MI, et al. Antihepatotoxic effect of marrubium vulgare and withania somnifera extracts on carbon tetrachloride-induced hepatotoxicity in rats. Journal of basic and clinical pharmacy. 2010;1(4):247-54. 3. Hussain L, Ikram J, Rehman K, Tariq M, Ibrahim M, Akash MSH. Hepatoprotective effects of Malva sylvestris L. against paracetamol-induced hepatotoxicity 2014. 396-402 p. 4. Favier A. The oxidative stress: concept and experimental interest to understand diseases mechanisms and therapeutic approaches. Actualité Chimique. 2003(11-12):108-15. 5. Sreelatha S, Padma PR, Umadevi M. Protective effects of Coriandrum sativum extracts on carbon tetrachloride-induced hepatotoxicity in rats. Food and Chemical Toxicology. 2009;47(4):702-8. 6. Murugesan GS, Sathishkumar M, Jayabalan R, Binupriya AR, Swaminathan K, Yun SE. Hepatoprotective and curative properties of Kombucha tea against carbon tetrachloride-induced toxicity. Journal of microbiology and biotechnology. 2009;19(4):397-402. 7. Chothani DL, Vaghasiya HU. A review on Balanites aegyptiaca Del (desert date): phytochemical constituents, traditional uses, and pharmacological activity. Pharmacognosy reviews. 2011;5(9):55-62. 8. Tata Kadiatou T, André T, Noufou O, Nogma Ernest S, Jotham Yhi-pene Nd, Salfo O, et al. Ethnopharmacological plants used to treat hepatitis and their anti-oxidant activity of district of Bobo-Dioulasso (Burkina Faso). International journal of Pharmacological Research. 2018;8:15. 9. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. PAIN. 1983;16(2):109-10. 10. OCDE. Toxicité orale aiguë - Méthode par classe de toxicité aiguë. . Lignes directrices de l’OCDE pour les essais de produits chimiques 2001. 11. Nitiema M, Sylvain I, Lazare B, Geoffroy Gueswindé O, Salfo O, Noufou O, et al. Acute and sub-acute toxicity studies of aqueous decoction of the trunk barks from Lannea microcarpa engl. And k. Krause (Anacardiaceae) in rodents 2018. 12. Singh J, Kakkar P. Antihyperglycemic and antioxidant effect of< i> Berberis aristata root extract and its role in regulating carbohydrate metabolism in diabetic rats. Journal of ethnopharmacology. 2009;123(1):22-6. 13. Beers RF, Jr., Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. The Journal of biological chemistry. 1952;195(1):133-40. 14. Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. The Journal of biological chemistry. 1972;247(10):3170-5. 15. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry. 1979;95(2):351-8. 16. Bryan NS, Grisham MB. Methods to detect nitric oxide and its metabolites in biological samples. Free radical biology & medicine. 2007;43(5):645-57. 17. Unies N. Système Général Harmonisé de classification et d’étiquetage des produits chimiques (SGH). Quatrième édition révisée. New York et Genève. ST/SG/AC.10/30/Rev4. : Nations unies; 2011. 18. Mukherjee PK. Quality control of herbal drugs. Business Horizons, New Delhi. 2002:543-5. 19. Eidi A, Mortazavi P, Moghadam JZ, Mardani PM. Hepatoprotective effects of Portulaca oleracea extract against CCl4-induced damage in rats. Pharmaceutical Biology. 2015;53(7):1042-51. 20. Nchouwet ML, Wansi N, Kodjio N, Poualeu K, Nkeng-Efouet A, Kamanyi A. Hepatoprotective and antioxidant effect of stem barks extracts: methanolic and aqueous of Pseudocedrela kotschyi (Meliaceae) on paracetamol-induced hepatic damage in rats. Asian J Biomed Pharm Sci. 2017;7:1-9. 21. Wang R, Yang Z, Zhang J, Mu J, Zhou X, Zhao X. Liver Injury Induced by Carbon Tetrachloride in Mice Is Prevented by the Antioxidant Capacity of Anji White Tea Polyphenols. Antioxidants. 2019;8(3):64. 22. Al-Olayan EM, El-Khadragy MF, Aref AM, Othman MS, Kassab RB, Abdel Moneim AE. The potential protective effect of Physalis peruviana L. against carbon tetrachloride-induced hepatotoxicity in rats is mediated by suppression of oxidative stress and downregulation of MMP-9 expression. Oxidative medicine and cellular longevity. 2014;2014:381413. 23. Suky TMG, Parthipan B, Kingston C, Mohanand P, Soris T. Hepatoprotective and antioxidant effect of Balanites aegyptiaca (L.) Del against CCl4 induced hepatotoxicity in rats. International Journal of Pharma Sciences and Research. 2011;2(4):887-92. 24. Ali BH, Bashir AK, Rasheed RA. Effect of the traditional medicinal plants Rhazya stricta, Balanitis aegyptiaca and Haplophylum tuberculatum on paracetamol-induced hepatotoxicity in mice. Phytotherapy Research. 2001;15(7):598-603. 25. Khan RA, Khan MR, Sahreen S. CCl 4-induced hepatotoxicity: protective effect of rutin on p53, CYP2E1 and the antioxidative status in rat. BMC complementary and alternative medicine. 2012;12(1):178. 26. Liu W, Wang Z, Hou JG, Zhou YD, He YF, Jiang S, et al. The Liver Protection Effects of Maltol, a Flavoring Agent, on Carbon Tetrachloride-Induced Acute Liver Injury in Mice via Inhibiting Apoptosis and Inflammatory Response. Molecules (Basel, Switzerland). 2018;23(9).