A novel investigation of in vitro anti-inflammatory and antioxidant activity of petroleum ether and chloroform extracts of Ficus krishnae stem bark
Keywords:
Ficus krishnae, Stem bark, Antioxidant, Anti-inflammatory, DPPH, ABTS, Phosphomolybdenum, Ferric reducing assayAbstract
There is great importance of the genus Ficus among the living beings since time immemorial. Besides aesthetic and religious values, many species of this genus are of use in day to day life as food and in traditional medicine. Ficus krishnae belongs to the family Moraceae, which is one of the oldest plant native to India. The plant extract is used as traditional medicine for healing various diseases. The main aim of the present study was to investigate the in-vitro anti inflammation and antioxidant potential of petroleum ether and chloroform extracts of Ficus krishnae stem bark. In vitro anti-inflammatory studies were performed for the extracts of petroleum ether and chloroform using protein denaturation inhibition assay and antioxidant activity was screened by 2, 2 diphenyl-1- picrylhydrazyl (DPPH) radical scavenging assay, ABTS, Phosphomolybdenum and reducing power assay. Finally percentage of inhibition of free radical and IC50 were calculated by the help of Statistical analysis. The results of present data has shown that the Chloroform extract of Ficus krishnae stem bark has contributed in causing high potential in-vitro anti inflammation and antioxidant activity fallowed by petroleum ether extract.
References
Gerard J Tortora, Sandra Reynolds, eds. Principles of Anatomy and Physiology. 2) Sies.H. Oxidative stress: Oxidants and antioxidants, Exp-Physiol, 1997, 82(2), pg : 291- 295. 3) Davies.K. Oxidative stress: the paradox of aerobic life, Biochem Soc Symp, 1995, 61, pg : 1-35 4) Valko.M., Leibfritzn.D., Moncol.J., Cronin.M., Mazur.M , Telser.J., Free radicals and Antioxidants in normal physiological functions and human disease, Int J Biochem Cell Biol, 2007, 39(1), pg: 44-48. 5) Stohs .S.,Bagchi. D., Oxidative mechanisms in the toxicity of metal ions, Free RadicBiolMed , 1995, 18 (2), pg : 321–336. 6) Nakabeppu..Y., Sakumi.K., Sakamoto.K., Tsuchimoto.D., Tsuzuki.T., Nakatsu.Y., Mutagenesis and carcinogenesis caused by the oxidation of nucleic acids, BiolChem, 2006, 266(1-2), pg : 37-56. 7) Sajida Begum, I. C. Barua, Ficus Species and its Significance.International Journal of Computer Applications in Engineering Sciences, 2012, vol II, 2231-4946. 8) Anonymous. Wealth of India: Raw Materials Vol. IV. New Delhi: CSIR; 1956, p. 25. 9) Biswas K. Observations on the systematic position of Ficuskrishnae. Curr Sci. 1934; 3(8): 424-7. 10) Kirtikar KR, Basu BD. Indian Medicinal Plants Vol. III. Dehradun: International Book distributors; 2005, p. 2312. 11) G. Leelaprakash, S. Mohan Dass“Invitro Anti-Inflammatory activity of Methanol extract of EnicostemmaAxillare”, Int. J. Drug Dev. & Res, 2011; 3(3): 189-196. 12) Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. International Journal of Pharma and Pharmacological Sciences 2010; 2(1):146-155. 13) Das Moonjit, MalipeddiHimaja, N. ArunaiNambiraj and Reshma Rajan Phytochemical analysis, Antioxidant activity and inVitro growth inhibition of struvite crystals byIpomoea eriocarpaleaf extractsJournal of Food Biochemistry 2015:1745–4514 14. Huang, H.B. Ke, J.S.He, X.Q.Ban, H.Zeng, Y.W.Wang. Extracts of Halenia elliptica exhibit antioxidant properties in vitro and in vivo. Food and Chemical Toxicology 49(2011) 185–190. 15) P Prieto, M Pineda, M Aguilar. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem. 269 (1999) 337–341. 16. Jungmin Oh, Heonjoo Jo, Ah Reum Cho, Sung-Jin Kim, Jaejoon Han. Antioxidant and antimicrobial activities of various leafy herbal teas. Food Control 31 (2013) 403-409. 17. Chidambara Murthy, K.N., Vanitha, A., Mahadeva Swamy, M. and Ravishankar, G.A. 2003. Antioxidant and Antimicrobial Activity of Cissus quadrangularis L. J Med Food, 6(2): 99-105 18. Sindhu Mathew, T. Emilia Abraham In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food and Chemical Toxicology.2006;44: 198–206 19. Ramesh L Londonkar, Aruna L H, Amarvani P Kanjikar,.Potential Investigation of In Vitro Antioxidant, Anti-Inflammatory and Anti-Haemolytic Activities from Polar Solvent Extracts of Pterocarpus marsupium. International Journal of Pharmacognosy and Phytochemical Research, 2017; 9(1); 100-107
