Pharmacognostic and Phytochemical screening of Cowpea seeds (Vigna unguiculata)

Authors

  • Ramesh L Londonkar Department of Biotechnology, Gulbarga University, Gulbarga-585106, Karnataka, India.
  • Basavarajeshwari S Awanti Department of Biotechnology, Gulbarga University, Gulbarga-585106, Karnataka, India.

Keywords:

Vigna unguiculata, Phytochemical screening, Pharmacognostic studies, Total phenol, Flavonoid content

Abstract

Vigna unguiculata (Cow pea) is well known medicinal plant. The drug isolated from this plant is used in different science of medicines like, ayurveda, unani, sidda, allopathic, homeopathic and naturopathic therapy. The present investigation deals with pharmacognostic and phytochemical screening of the seeds of Vigna uguiculata. The pharmacognostic studies include physicochemical constant and fluorescence analysis. The petroleum ether, chloroform, methanol and aqueous extracts are subjected to preliminary phytochemical screening. The physicochemical constants obtained were found to be within normal levels prescribed by phytochemical standards. The phytochemical studies of seeds of Vigna uguiculata revealed the presence of primary and secondary metabolites like proteins, carbohydrates, glycosides, phenols,flavonoids, saponins, sterols and alkaloids. The flavonoid content of methanol extract was found to be 621± 2.494 µg rutin/g. These studies provide a referential information for identification of this crude drug and the results are helpful for the isolation of medicinally important active components.

References

. Priya S, Santhiya S. Phytochemical

properties and antibacterial activity of

allotropic procera extracts against

human pathogens.Adv pharamacol

Toxicol 2011; 12(2):21-24.

. Gokhale SB. Textbook of

Pharmacognosy, Nirali Prakashan,

Pune,(1979).

. Mukherjee PK, Quality Control of

Herbal Drugs, an Approach to

evaluation of Botanicals, Business

Horizons Pharmaceutical Publishers,

New Delhi (2002), pp 186.

. Raghunathan K. Pharmacopoeial

standards for Ayurvedic formulations.

Central Council for Research in Indian

Medicine and Homoeopathy, New

Delhi, India ,(1976), pp. 42.

. Trease G.E, Evans WC.

Pharmacognosy, Harcourt brace & Co.

Asia, Pvt. Ltd., W.B. Saunders

Company Ltd., 15th edition, 2002.

. Kadam PV, Yadav KN, Narappanawar

NS, Shivatare RS, Bhusnar HU, Patil

MJ. Pharmacognosy Journal, 2011,

(26), 19-24.

. Summerfield RJ, Huxley PA, Stelle W.

Cowpea (Vigna unguiculata L. Walp).

Field Crop Abstr 1974; 27: 301ă312.

. Duke JA. Introduction to food legumes,

IN: S. R. Singh (Ed.). Insect pests of

tropical food legumes 1990; pp1ă42

(Chichester, United Kingdom: John

Wiley & Sons).

. Chopra RN, Nayar SL, Chopra IC.

Glossary of Indian medicinal plant 1986

(including the supplement), (New Delhi:

Council of Scientific and Industrial

Research).

. Van Wyk BE & Gericke N. People's

plants: A guide to useful plants of

Southern Africa 2000; pp. 192

(Pretoria, South Africa: Briza

Publications).

. Kritzinger Q, Lall N, Aveling TAS.

Antimicrobial activity of cowpea (Vigna

unguiculata) leaf extracts. South

African Journal of Botany 2005; 71:

ă48.

. Indian Pharmacopoeia,Government of

India, Ministry of Health and Family

Welfare,Controller of Publication, 4th

ed, New Delhi, 4(II);1996, A53-A54.

. WHO/PHARM/92.559/rev.1, Quality

Control Methods for Medicinal Plant

Materials .Chase C R and Pratt R S.,

Fluorescence of Powdered Vegetable

drugs with particular reference to

Development of a System of

Identification. J Am Pharmacol

Assoc1949 ; 38:32.

. Kokoshi CJ, Kokoshi RJ and Sharma

FT. Fluorescence of powdered

vegetablerugs under Ultraviolet

radiation. J Pharm Asses 1958;

:715-717.

. Kokate CK, Khandelwal KR, Pawar AP,

Gokhale SB. Practical

Pharmacognosy,Nirali Prakashan 1995

; 1:11-19.

. Harborne JB. Methods of extraction

and isolation In: Phytochemical

methods, 3rd Ed., Chapman and Hall,

London1998; 60-66.

. Raghuramulu N, Madhava Nair K,

Kalyanasundaram S. A manual of

Laboratory techniques: National

Institute of Nutrition, Hydrabad 2003; p:

-195.

. Alfawaz MA. Chemical composition of

hummayd (Rumex vesicarius) grown in

Saudi Arabia. Journal of food

composition and analysis 2006;

:552-555.

. Filho JMB, Alencar AA, Nunes XP,

Tomaz AC, Filho SJG, Petronio FA,

Silva MS, Souza MFV, and Cunha

EVL. (2008). Source of alpha-, beta-,

gamma-, delta-, and epsiloncarotenes: A twententieth century

review. Brazillian Journal of

Pharmacognosy, 18(1) 135-134.

. African Pharmacopoeia. General

methods for analysis Is ted. 2:

(OAU/STRC) Logos. 1986; p.123.

. Musa KY, Katsayal AU, Ahmed A,

Mohammed Z, Danmalam UH.

Pharmacognostic investigation of the

leaves of Gisekia pharmacioides.

African J. Biotech. 2006; 956-957.

. Kadam PV, Yadav KN, Deoda RS,

Narrappanawar NS, Shivtare RS, Patil

MJ. Pharmacognostic and

Phytochemical Studies on Roots of

Agave Americana (Agavaceae).

International Journal of

Pharmacognosy & phytochemical

Reasearch 2012;4(3):92-96.

. Pimenta AM, Montenegro MC, Araujo

AN, Martinez JC. 2006. Application of

sequential injections analysis to

pharmaceutical analysis. J Pharmaceut

Biomed 40: 16-34.

. Mohan Kalaskar G, Sanjay Surana

J.Pharmacognostic and phytochemical

studies on Ficus Microcarpa L.fil. Anc

Sci Life 2012; oct-dec 32(2): 107-111.

. Jimoh FO, Adedapo AA, Aliero AA and

Afolayan A. (2008). Polyphenolic

contents and biological activities

of Rumex ecklonianus. Pharm. Biol.,

: 333-340.

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Published

31-12-2014

How to Cite

1.
Ramesh L Londonkar, Basavarajeshwari S Awanti. Pharmacognostic and Phytochemical screening of Cowpea seeds (Vigna unguiculata). ijp [Internet]. 2014 Dec. 31 [cited 2024 Oct. 23];6(4):510-4. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/364

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