Antiviral activity and Antioxidant role of phenolics from Sophora interrupta Bedd in NDV induced oxidative stress in chickens
Keywords:
Newcastle Disease Virus, Sophora interrupta Bedd, Antioxidant enzymes, Brain, Lung, ChickenAbstract
The present investigation is taken up to evaluate the antiviral efficacy of phenolics isolated from Sophora interrupta Bedd and their antioxidant role in the brain and lungs of chicken during Newcastle disease virus (NDV) induced oxidative stress. The activity levels of selected antioxidant enzymes such as superoxide dismutase (SOD), catalyse (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) levels were significantly decreased in brain and lung tissues of NDV infected animals over controls causing oxidative stress. In addition, histopathological alterations disclosed that lungs of NDV infected chicken were affected severely as evidenced by the alterations in alveolar cell morphology, congestion, necrotic and degenerative changes whereas degeneration of Purkinje cells, neuronal necrosis, degeneration in myelin sheath and compression of cells were observed in the brain of NDV infected chickens. These reduced antioxidant defence mechanisms and histopathological abnormalities were restored to normal when chicken were pre-treated with the phenolics isolated from Sophora interrupta Bedd at the dose of 300 mg/Kg Bw/day for one week. Pre-treatment with the phenolics isolated from the above medicinal plant also caused significant reduction in the titre levels of NDV. These results suggest that pre-treatment with the phenolics isolated from Sophora interrupta Bedd exhibited significant antiviral activity and thus the plant extract may be used as a prophylactic treatment for the prevention of NDV infection in chicken.
References
. Peterhans E. Sendai virus stimulates chemiluminescence in mouse spleen cells. Bioch.Biophy. Res. Comm. 1979; 91: 383–392.
. Peterhans E, Grob M, Burge T and Zanoni R. Virus-induced formation of reactive oxygen intermediates in phagocytic cells. Free Radical Res Comm. 1987; 3:39-46.
. Muller. Reactive oxygen intermediates and Human Immunodeficiency Virus (HIV) infection. Free. Radi. Biol. Med. 1992; 13: 651–657.
. Pasquier R, Olivier Y, Auclair C and Packer L. Oxidative Stress, Cell Activation and Viral Infection. 1994; (Conference).
. Ravindra BK, Koteswar RV, Nanda KY, Kishore P, Subbaiah KCV, Bhaskar M, Lokanatha V and Nagaraju C. Identification of substituted [3, 2-a] pyrimidines as selective antiviral agents: 3 Molecular modeling studies. Antiviral. Res. 2012; 95: 118–127.
. Akaike T Role of free radicals in viral pathogenesis and mutation. Rev. Med. Virol. 2001; 11:87-101.
. Lee Y, Galoforo SS, Berns CM, Chen JC, Davis BH, Sim JE, Corry PM and Spitz DR. Glucose deprivation-induced cytotoxicity and alterations in mitogen-activated protein kinase activation are mediated by oxidative stress in multidrug-resistant human breast carcinoma cells. J Biol Chem. 1998; 273: 5294-5299.
. Sies H, Sharov VS, Klotz L and Briviba K. Glutathione peroxidise protects against peroxinitrite-mediated oxidations. A new function for seleno proteins as peroxynitrite reductase. J Biol Chem. 1997;272: 27812-27817.
. Davies K Oxidative stress: The paradox of aerobic life. Bio chem Soc Symp. 1995; 61: 1-31.
. Hennet T, Peterhans E and Stocker R Alterations in antioxidant defences in lung and liver of mice infected with Influenza A virus. J Gen Virol. 1992; 73: 39-46.
. Benzer F and Yilmaz S Effects on oxidative stress and antioxidant enzyme activities of experimentally induced Ornithobacterium rhinotracheale infection in broilers. J Ani Vet Adv. 2009; 8 (3): 548-553.
. Peterhans E, Grob M, Burge T and Zanoni R. Virus-induced formation of reactive oxygen intermediates in phagocytic cells. Free Radical Res Comm. 1987; 3:39-46.
. Akaike M oxygen free radicals as pathogenic molecules in viral diseases. Proc. Soc. Expt. Biol. Med. 1991;198: 721-729.
. Ikeda T, Shimokata K and Daikoku T. Pathogenesis of cytomegalovirus-associated Pneumonitis in ICR mice: possible involvement of superoxide radicals. Arch Virol. 1992; 127: 11-24.
. Schwarz B. Oxidative stress during viral infection: A review. Free. Radical. Biol. Med. 1996; 21:641-649.
. Liang R, Guo KK, Yi L, Zhang YX, Li JQ and Yue ZQ. Study of Chinese herbal medicinal immune stimulators on immune enhancement in chickens. Chin J Vet Sci Technol. 1998; 28: 11-13.
. Liu JG, Hu YL, Chen YK, Zhang BK and Kong XF. The safe concentrations of several components in natural drugs on CEF. Prog Vet Med. 2002; 23: 88-91.
. Hu YL. Progress in the study of immunopharmocology of Chinese herbal medicine. Chi. J Immunol. 1997;13: 96-98.
. Ma SC, Du J, But PP, Deng XL, Zhang YW, Ooi VE, Xu HX, Lee SH and Lee SF Antiviral Chinese medicinal herbs against Respiratory Syncytial Virus. J Ethnopharma. 2002; 79 (2): 205-211.
. Ma D, Shan A, Chen Z, Du J, Song K, Li J and Xu Q. Effect of Ligustrum lucidum and Schisandra chinensis on the egg production, antioxidant status and immunity of laying hens during heat stress. Arch Anim Nut. 2005; 59 (6): 439-47.
. Guye FHE. Ethno-veterinary medicine against poultry diseases in African villages. World Poult Sci J. 1999; 55: 187-198.
. Singh UP, Sarma BK, Singh DP and Bahadur A Plant growth-promoting Rhizobacteria-mediated induction of Phenolics in Pea (Pisum sativum) after infection with Erysiphe pisi. Current Microbiology. 2002; 44, 396-400.
. Reed, LJ and Muench H A simple method of estimating fifty per cent endpoints. Am. J. Hyg. 1938; 27: 493–497.
. Misra HP and Fridovich I The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol Chem. 1972; 247:3170–3175.
. Aebi H and Packer L Catalase in vitro. Methods in Enzymol. 1984; 105: 121-126.
. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG and Hoekstra WG Selenium: Biochemical roles as a component of glutathione peroxidase. Science. 1973; 19:588–590.
. Carlberg I and Mannervik B Glutathione reduction. In: Methods in Enzymology. Academic Press in Orlando. 1985; 113: 484-499.
. Habig WH, Pabst MJ and Jakoby WB Glutathione-s-transfarases. J. Biol, Chem. 1974; 249: 7130-7139.
. Yashoda M, Tianshung L, Castro SM, Garofalo RP and Casola A Respiratory synctial virus induces oxidative stress by modulating antioxidant enzymes. Am J Respr Cell Mol. 2009; 41: 348-357.
. Waihenya RK, Mtambo MMA, Nkwengulila G and Minga UM Efficacy of the crude extract of Aloe secundflora against Salmonella gallinarum in experimentally infected free range chickens in Tanzania. J of Ethnopharmacol. 2002; 79 (3): 317-323.
. Marizvikuru M, Evison B, Michael C and Tinyiko EH. Use of herbal plants in poultry health management in the Mushagashe small-scale commercial farming area in Zimbabwe. Int J Appl Res Vet Med. 2005; 3(2): 2005.
. Kemp MC, Kahlon JB, Chinnah AD, Carpenter RH, McAnalley BH, McDaniel HK and Shannon WM. In vitro evaluation and the antiviral effects of acemannan on the replication and pathogenesis of HIV-1 and other enveloped viruses: modification of the processing of glycoprotein precursors. Antiviral Res. 1990; 13: 83.
. Kong XF, Hu YL, Yin YL, Wu GY, Rui R, Wang DY and Yang CB Chinese herbal ingredients are effective immune stimulators for chickens infected with the Newcastle disease virus. Poul Sci. 2006; 85: 2169-2175.
. Wegner U, Mundt S, Kliefoth C, Meyer B, Lindequist U and Mentel R Antiviral activity of plant polyphenols against Influenza virus. Hyg Med. 2000; 25(3): 75-78.
. Moreki JC. Small-scale poultry production systems in Serowe-Palapye Subdistrict (Botswana).M.Sc. Thesis, University of Melbourne, Melbourne (1997) Australia.
. Peter FS. Silymarin as a Natural Antioxidant: An Overview of the Current Evidence and Perspectives. Antioxidants. 2015; 4: 204-247.
. Nadia MH, El-Sabbagh AH, El-Assily S, Khashaba E, El-Bordini F, El-Ebiary AE and Taha MM. Virucidal effect of Garlic and Onion oily extracts on different strains of Newcastle disease virus. Ben Vet Med Res. 1991; 1(1): 205-214.
. Hegazi AG, El-Berdiny F, El-Assily S, Khashaba E, Hassan N and Popov S. Studies of some aspects of antiviral activity-Influence of Propolis on Newcastle disease virus. Vet. Med. J. Giza. 1993; 41(2): 53-56.
. Hegazi AG, El-Berdiny F, El-Assily S, Khashaba S and Abd-El-Hady F Studies of some aspects of antiviral activity-Influence of Solenostemma argle on Newcastle disease virus. Vet. Med. J. Giza. 1994; 54(5): 435-441.
. Singh RK, Singh DP and Pathak RC. Antiviral activity of indigenous plants. Indian J Vet Res. 1994; 3(2): 10-19.
. Subbaiah KCV, Raniprameela D, Visweswari G, Rajendra W and Lokanatha V. Perturbations in the antioxidant metabolism during Newcastle disease virus (NDV) infection in chicken-Protective role of vitamin E. Naturwissenschaften. 2011; 98: 1019-1026.
