Consumption of Moringa oleifera flour and its effects on the biochemical profile and intestinal motility in an animal model

Authors

  • Elen Landgraf Guiguer Medical School of Marilia - UNIMAR, Marília, São Paulo, Brazil and School of Food Technology of Marilia (FATEC), São Paulo, Brazil
  • Sandra Maria Barbalho 1Medical School of Marilia - UNIMAR, Marília, São Paulo, Brazil 2School of Food Technology of Marilia (FATEC), São Paulo, Brazil
  • Paulo Sergio Marinelli 1Medical School of Marilia - UNIMAR, Marília, São Paulo, Brazil 2School of Food Technology of Marilia (FATEC), São Paulo, Brazil
  • Patricia Cincotto Bueno 1Medical School of Marilia - UNIMAR, Marília, São Paulo, Brazil 2School of Food Technology of Marilia (FATEC), São Paulo, Brazil
  • Patricia Cincotto Bueno Medical School of Marilia - UNIMAR, Marília, São Paulo, Brazil
  • Claudemir Gregorio Mendes Medical School of Marilia - UNIMAR, Marília, São Paulo, Brazil
  • Mirele Cristina Santos Medical School of Marilia - UNIMAR, Marília, São Paulo, Brazil
  • Leticia Maria Salzedas Medical School of Marilia - UNIMAR, Marília, São Paulo, Brazil
  • Marie Oshiiwa 1Medical School of Marilia - UNIMAR, Marília, São Paulo, Brazil 2School of Food Technology of Marilia (FATEC), São Paulo, Brazil
  • Manoel Lima Mendes Faculty of Sciences – UNESP, Av Engenheiro Luiz Edmundo Carrijo Coube, 14-01, 17033-360, Bauru, SP, Brazil.
  • Claudia Teixeira Nicolau School of Food Technology of Marilia (FATEC), São Paulo, Brazil
  • Alda Maria Otoboni School of Food Technology of Marilia (FATEC), São Paulo, Brazil

Keywords:

Moringa oleifera, glycaemia, intestinal motility

Abstract

Moringa oleifera (MO) belongs to the family Moringaceae and is native to tropical Africa. It exhibits many therapeutic properties and has been widely cultivated because of the high food value of the leaves, fruit, flowers and roasted seeds. It possesses high quality protein, calcium, iron, fiber, minerals and essential amino acids. The objective of this study was to evaluate the effects of MO flour in anthropometric and biochemical profile of Wistar rats. Animals were divided randomly into the following groups (n=10): G1 (control group) and G2 (treated with MO flour mixed to the rat food) were both treated for 40 days and G3 (control group), G4 (treated with senne) and G5 (treated with MO flour) were studied to evaluate the intestinal motility. After 40 days, animals of G1 and G2 were euthanized and evaluation of glycaemia, total cholesterol, triglycerides, LDL-c, VLDL-c, HDL-c, C reactive Protein (PCR), hepatic enzymes, Lee Index, weight and visceral fat were performed. Our results showed reduction of visceral fat, total cholesterol, triglycerides, LDL-c, and VLDL-c and increase in the HDL-c levels. No significant differences were found in the body weight, glycaemia, hepatic enzymes and PCR. The MO flour also promoted laxative effects similar do senne. Our results with the use of Moringa oleifera flour are very promising, once its use improved lipid profile, prevented weight gain and showed no adverse effects. Thus we may conclude that this flour could be added to industrial products in order to provide healthier products to the consumers.

References

. Araújo AC, Guiguer ÉL, Barbalho SM, Bueno PC, Lopes JA, da Silva BF, Girotto LC, de Paula MG, Zeber PV, de Alvares Goulart R. Phytochemical Characteristics of Seeds and Its Effects on the Intestinal Motility and Toxicity of Joannesia princeps. J Med Food. 2016 Jan;19(1):68-72. doi: 10.1089/jmf.2015.0071.

. Azimi P, Ghiasvand R, Feizi A, Hosseinzadeh J, Bahreynian M, Hariri M, Khosravi-Boroujeni H. Effect of cinnamon, cardamom, saffron and ginger consumption on blood pressure and a marker of endothelial function in patients with type 2 diabetes mellitus: A randomized controlled clinical trial. Blood Press. 2016 Jan 12:1-8.

. Kavitha S, John F, Indira M. Amelioration of inflammation by phenolic rich methanolic extract of Ocimum sanctum Linn. leaves in isoproterenol induced myocardial infarction. Indian J Exp Biol. 2015 Oct;53(10):632-40.

. Khalafalla MM, Abdellatef E, Dafalla HM, Nassrallah AA, Aboul-Enein KM, Lightfoot DA, et al. Active principle from Moringa oleifera Lam leaves effective against two leukemias and a hepatocarcinoma. Afr J Biotechnol.2010;9:8467–71.

. Mansour HH, El Azeem MGA, Ismael NE. Protective Effect of Moringa oleifera on γ-Radiation-Induced Hepatotoxicity and Nephrotoxicity in Rats.American Journal of Phytomedicine and Clinical Therapeutics. 2014;2:495–508.

. Silva JC, Marques RG, Teixeira BEM, Ciabotti S. Determinação da composição química das folhas de Moringa oleífera lam. (Moringaceae). Available at: http://www.iftm.edu.br/proreitorias/pesquisa/revista/pdf/Resumo_10.pdf>.

. Bin-Meferij MM1, El-Kott AF2. The radioprotective effects of Moringa oleifera against mobile phone electromagnetic radiation-induced infertility in rats. Int J Clin Exp Med. 2015 Aug 15;8(8):12487-97.

. Tiloke C, Phulukdaree A, Chuturgoon AA. The antiproliferative effect of Moringa oleifera crude aqueous leaf extract on cancerous human alveolar epithelial cells. BMC Complement Altern Med. 2013;13:226.

. Fakurazi S, Hairuszah I, Nanthini U. Moringa oleifera Lam prevents acetaminophen induced liver injury through restoration of glutathione level.Food Chem Toxicol. 2008;46:2611–5. [PubMed]

. Jaiswal D, Rai PK, Mehta S, Chatterji S, Shukla S, Rai DK, Sharma G, Sharma B, Khair S, Watal G. Role of Moringa oleifera in regulation of diabetes-induced oxidative stress. Asian Pac J Trop Med. 2013;6:426–32.

. Verma AR, Vijayakumar M, Mathela CS, Rao CV. In vitro and in vivo antioxidant properties of different fractions of Moringa oleifera leaves. Food Chem Toxicol. 2009;47:2196–201.

. Das N, Ganguli D, Dey S. Moringa oleifera Lam. seed extract prevents fat diet induced oxidative stress in mice and protects liver cell-nuclei from hydroxyl radical mediated damage. Indian J Exp Biol. 2015 Dec;53(12):794-802

. Instituto Adolfo Lutz (IAL). Physical and chemical methods for food analysis. Sao Paulo, Brasil, 2008.

. Schulpis K, Karikas GA. Serum cholesterol and triglyceride distribution in 7767 school-aged Greek children. Pediatrics. 1998 May;101(5):861-4.

. Munshi RP, Joshi SG1, Rane BN. Development of an experimental diet model in rats to study hyperlipidemia andinsulin resistance, markers for coronary heart disease. Indian J Pharmacol. 2014 May-Jun;46(3):270-6. doi: 10.4103/0253-7613.132156.

. Bernardis LL, Patterson BD: Correlation between ‘Lee Index’ and carcas fat content in weanling and adult female rats with hypothalamic lesions. J Endocrinol 1968; 40: 527-528.

. Nery CS; Pinheiro IL; Muniz GS; Vasconcelos DAA; França SP; Nascimento E. Medidas murinométricas e eficiência alimentar em ratos provenientes de ninhadas reduzidas na lactação e submetidos ou não ao exercício de natação. Rev Bras Med Esporte 2011; 17 *:49-55.

. Michelin DC, Salgado HRN: Evaluation of the laxative activity of Operculina macrocarpa L. Urban (Convolvulaceae). Rev Bras Farmacogn 2004;14(2):15-109.

. Johnson RK. Reducing Intakes of Sugar Sweetened Beverages is Vital to Improving Our Nation's Health. Circulation. 2016 Jan 11. pii: Circulationa HA.115.020453. [Epub ahead of print]

. Hall ME, Brinkley TE, Chughtai H, Morgan TM, Hamilton CA, Jordan JH, Stacey RB, Soots S, Hundley WG. Adiposity Is Associated with Gender-Specific Reductions in Left Ventricular Myocardial Perfusion during Dobutamine Stress. PLoS One. 2016 Jan 11;11(1):e0146519. doi: 10.1371/journal.pone.0146519.

. Mossmann M, Wainstein MV, Gonçalves SC, Wainstein RV, Gravina GL, Sangalli M, Veadrigo F, Matte R, Reich R, Costa FG, Bertoluci MC. HOMA-IR is associated with significant angiographic coronary artery disease in non-diabetic, non-obese individuals: a cross-sectional study. Diabetol Metab Syndr. 2015 Nov 14;7:100. doi: 10.1186/s13098-015-0085-5.

. Olayaki LA, Irekpita JE, Yakubu MT, Ojo OO. Methanolic extract of Moringa oleifera leaves improves glucose tolerance, glycogen synthesis and lipid metabolism in alloxan-induced diabetic rats. J Basic Clin Physiol Pharmacol. 2015 Nov 1;26(6):585-93. doi: 10.1515/jbcpp-2014-0129.

. Park HR, Shin SR, Han AL, Jeong YJ. The Correlation between the Triglyceride to High Density Lipoprotein Cholesterol Ratio and Computed Tomography-Measured Visceral Fat and Cardiovascular Disease Risk Factors in Local Adult Male Subjects. Korean J Fam Med. 2015 Nov;36(6):335-340.

. Abd El Latif A, El Bialy Bel S, Mahboub HD, Abd Eldaim MA. Moringa oleifera leaf extract ameliorates alloxan-induced diabetes in rats by regeneration of β cells and reduction of pyruvate carboxylase expression. Biochem Cell Biol. 2014 Oct;92(5):413-9. doi: 10.1139/bcb-2014-0081.

. Kushwaha S, Chawla P, Kochhar A. Effect of supplementation of drumstick (Moringa oleifera) and amaranth (Amaranthus tricolor) leaves powder on antioxidant profile and oxidative status among postmenopausal women. J Food Sci Technol. 2014 Nov;51(11):3464-9. doi: 10.1007/s13197-012-0859-9.

. Chumark P, Khunawat P, Sanvarinda Y, Phornchirasilp S, Morales NP, Phivthong-Ngam L,Ratanachamnong P, Srisawat S, Pongrapeeporn KU. The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves. J Ethnopharmacol. 2008 Mar 28;116(3):439-46. doi: 10.1016/j.jep.2007.12.010.

. Adedapo AA, Falayi OO, Oyagbemi AA. Evaluation of the analgesic, anti-inflammatory, anti-oxidant, phytochemical and toxicological properties of the methanolic leaf extract of commercially processed Moringa oleifera in some laboratory animals. J Basic Clin Physiol Pharmacol. 2015 Sep;26(5):491-9. doi: 10.1515/jbcpp-2014-0105.

. Akunna GG, Ogunmodede OS, Saalu CL, Ogunlade B, Bello AJ, Salawu EO. Ameliorative effect of Moringa oleifera (drumstick) leaf extracts on chromium-induced testicular toxicity in rat testes. World J Life Sci Med Res.2012;2:20.

. Maida A, Farooq A, Raziya N, Umer R, Kazi T, Nadeem M. Mineral composition of Moringa oleifera leaves and pods from different regions of Punjab, Pakistan. Asian Journal of Plant Sciences. 2005

. Leone A, Fiorillo G, Criscuoli F, Ravasenghi S, Santagostini L, Fico G,Spadafranca A, Battezzati A, Schiraldi A, Pozzi F, di Lello S, Filippini S, Bertoli S Nutritional Characterization and Phenolic Profiling of Moringa oleifera Leaves Grown in Chad, Sahrawi Refugee Camps, and Haiti. Int J Mol Sci. 2015 Aug 12;16(8):18923-37. doi: 10.3390/ijms160818923.

. Vongsak B, Mangmool S, Gritsanapan W. Antioxidant Activity and Induction of mRNA Expressions of Antioxidant Enzymes in HEK-293 Cells of Moringa oleiferaLeaf Extract. Planta Med. 2015 Aug;81(12-13):1084-9. doi: 10.1055/s-0035-1546168. Epub 2015 Jul 10.

. Stohs SJ1, Hartman MJ. Review of the Safety and Efficacy of Moringa oleifera. Phytother Res. 2015 Jun;29(6):796-804. doi: 10.1002/ptr.5325. Epub 2015 Mar 24.

. Zeb I, Budoff M.Coronary Artery Calcium Screening: Does it Perform Better than Other Cardiovascular Risk Stratification Tools? Int J Mol Sci. 2015 Mar 23;16(3): 6606-6620.

. Kawada T, Otsuka T, Inagaki H, Wakayama Y, Katsumata M. Biological markers, lifestyles and metabolic syndrome in workers. Diabetes Metab Syndr, 2015; pii: S1871-4021(15)00022-3. doi: 10.1016/j.dsx.2015.02.009.

. Straatman J, de Weerdesteijn EW, Tuynman JB, Cuesta MA, van der Peet DL. C-Reactive Protein as a Marker for Postoperative Complications. Are There Differences in Emergency and Elective Colorectal Surgery? Dis Colon Rectum. 2016 Jan;59(1):35-41.

. Cai C, Cao Z, Loughran PA, Kim S, Darwiche S, Korff S, Billiar TR. Mast cells play a critical role in the systemic inflammatory response and end-organ injury resulting from trauma. J Am Coll Surg. 2011;213(5):604-615.

. Wald A. Constipation: Advances in Diagnosis and Treatment. JAMA. 2016;315(2):185-91. doi: 10.1001/jama.2015.16994.

. Mounsey A, Raleigh M, Wilson A. Management of Constipation in Older Adults. Am Fam Physician. 2015;92(6):500-4.

. Cirillo C, Capasso R. Constipation and Botanical Medicines: An Overview. Phytother Res. 2015 Oct;29(10):1488-93. doi: 10.1002/ptr.5410.

. Li H, Li C, Zhang C, Chen B, Hui L, Shen Y. Compositional and gastrointestinal prokinetic studies of Pugionium (L.). Food Chem. 2015 Nov 1;186:285-91. doi: 10.1016/j.foodchem.2015.03.146. Ep

. Yenidogan E, Okan I, Kayaoglu HA, Akgul GG, Sansal M, Tali S, Ozsoy Z, Sahin M. Same-day colonoscopy preparation with Senna alkaloids and bisacodyl tablets: a pilot study. World J Gastroenterol. 2014 Nov 7;20(41):15382-6. doi: 10.3748/wjg.v20.i41.15382.

. Sun JW, Gu JN, Du P, Chen W. Comparison of two types of colectomy in treating slow transit constipation with or without melanosis coli. World J Gastroenterol. 2015 Sep 7;21(33):9736-40. doi: 10.3748/wjg.v21.i33.9736.

. Cowley K, Jennings HW, Passarella M. Who Turned Out the Lights? An Impressive Case of Melanosis Coli. ACG Case Rep J. 2015 Oct 9;3(1):13-4. doi: 10.14309/crj.2015.85.

. Abendroth A, Klein R, Schlaak J, Metz KA, Dobos GJ, Langhorst J. Impressive picture of a melanosis coli after chronic anthraquinone laxative use--is there an increased risk for colorectal cancer? Z Gastroenterol. 2009;47:579–582

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Published

30-09-2016

How to Cite

1.
Elen Landgraf Guiguer, Sandra Maria Barbalho, Paulo Sergio Marinelli, Patricia Cincotto Bueno, Patricia Cincotto Bueno, Claudemir Gregorio Mendes, Mirele Cristina Santos, Leticia Maria Salzedas, Marie Oshiiwa, Manoel Lima Mendes, Claudia Teixeira Nicolau, Alda Maria Otoboni. Consumption of Moringa oleifera flour and its effects on the biochemical profile and intestinal motility in an animal model. ijp [Internet]. 2016 Sep. 30 [cited 2024 Nov. 21];8(3):427-34. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/488

Issue

Vol. 8 No. 3 (2016): Jul- Sep

Section

Original Research Articles

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