Acute Oral Toxicity Study of Paris polyphylla extract in Rats
Keywords:Paris polyphylla, Rhizome, Hydroalcoholic Extract, Cytotoxic
Paris polyphylla Smith is regarded as “Wonder Herb” due to its vast therapeutic applications ranging from diarrhea to cancer. The cytotoxic effect of the rhizome extract on the healthy rats was monitored over a period of time. The extract proved non cytotoxic as animals gained weight and no signs of mortality, ill health or overt toxicity were observed. A brief period of dullness indicates a possible role of the extract in neuro-suppression. The cutoff median lethal dose (LD50) of extract after single oral administration to female rats, observed over a period of 14 days is found to be greater than 5000 mg/Kg body weight. Thus it can be used in the treatment of neurological disorders associated with hyper active neurons. Moreover as the extract is cytotoxic to cancerous cells only it would prove good target for future studies on anti cancer drugs. The results of this study collectively specify that oral administration of Paris polyphylla is not connected with any toxicologically significant effects and the data could provide satisfactory preclinical evidence of safety to launch a clinical trial on a standardized formulation of the plant extracts.
Pande PC, Tiwari L, Pande HC. Ethnoveterinary plants of Uttaranchal — A review. Ind J Trad Knowledge. 2007; 6: 444-458.
Shah SA, Mazumder PB, Choudhury MD. Medicinal Properties of Paris polyphylla Smith:A REVIEW. J herbal med Toxicol. 2012; 6: 27-33.
Wang GX, Han J, Zhao LW, Jiang DX, Liu YT, Liu XL. Anthelmintic activity of steroidal saponins from Paris polyphylla. Phytomedicine. 2010; 17: 1102-1105.
Negi JS, Bisht VK, Bhandari AK, Bhatt VP, Singh P, Singh N. Paris polyphylla: chemical and biological prospectives. Anticancer Agents Med Chem. 2014; 14: 833-839.
Watanabe T, Rajbhandrai KR, Malla KJ, Yahara S. A Hand Book of Medicinal Plants of Nepal.Japan: Ayur Seed; 2005: 262.
Manandhar NP, Manandhar S. Plants and People of Nepal. Portland: Timber Press; 2002: 636.
Grieve M. A Modern Herbal, New York: Dover Publications Inc; 1971: 919.
Weisberg ME, Graham JR. A Barefoot Doctor's Manual: A Guide to Traditional Chinese and Modern Medicine, First edition. Canada: cloudburst press; 1977: 209.
Yeung H-C. Handbook of Chinese Herbs and Formulas. Second Printing edition. Los Angeles: Institute of Chinese Medicine; 1985
Devkota KP. Bioprospecting studies on Sarcococca hookeriana Bail, Sonchus wightianus DC, Paris polyphylla Smith and related Medicinal Herbs of Nepal: Ph D Thesis, HEJResearch Institute of Chemistry, International Centre for Chemical Science,. University of Karachi, Karachi: Pakistan; 2005.
Shen S, Chen D, Li X, Li T, Yuan M, Zhou Y, Ding C. Optimization of extraction process and antioxidant activity of polysaccharides from leaves of Paris polyphylla. Carbohydr Polym. 2014; 104: 80-86.
Ballabha, R, Singh D, Tiwari JK, Tiwari P. Diversity and availability status of ethno-medical plants in the lohba range of kedarnath forest division (KFD). Garhwal Himalayas. Global J Res Med Plants and Indige med. 2013; 2: 198-212.
Yu ZY, Guo L, Wang B, Kang LP, Zhao ZH, Shan YJ, Xiao H, Chen JP, Ma BP, Cong YW. Structural requirement of spirostanol glycosides for rat uterine contractility and mode of their synergism. J Pharm Pharmacol. 2010; 62: 521-529.
Guo L, Su J, Deng BW, Yu ZY, Kang LP, Zhao ZH, Shan YJ, Chen JP, Ma BP, Cong YW. Active pharmaceutical ingredients and mechanisms underlying phasic myometrial contractions stimulated with the saponin extract from Paris polyphylla Sm. var. yunnanensis used for abnormal uterine bleeding. Hum Reprod. 2008; 23: 964-971.
Qin XJ, Sun DJ, Ni W, Chen CX, Hua Y, He L, Liu HY. Steroidal saponins with antimicrobial activity from stems and leaves of Paris polyphylla var. yunnanensis. Steroids. 2012; 77: 1242-1248.
Zhu L, Tan J, Wang B, Guan L, Liu Y, Zheng C. In-vitro antitumor activity and antifungal activity of pennogenin steroidal saponins from Paris polyphylla var. yunnanensis. Iran J Pharm Res. 2011;10: 279-286.
Yan LL, Zhang YJ, Gao WY, Man SL, Wang Y. In vitro and in vivo anticancer activity of steroid saponins of Paris polyphylla var. yunnanensis. Exp Oncol. 2009; 31:27-32.
Wang Y, Zhang YJ, Gao WY, Yan LL. Anti-tumor constituents from Paris polyphylla var. yunnanensis]. Zhongguo Zhong Yao Za Zhi. 2007; 32:1425-1428.
Li Y, Gu JF, Zou X, Wu J, Zhang MH, Jiang J, Qin D, Zhou JY, Liu BX, Zhu YT, Jia XB, Feng L, Wang RP. The anti-lung cancer activities of steroidal saponins of P. polyphylla Smith var. chinensis (Franch.) Hara through enhanced immunostimulation in experimental Lewis tumor-bearing C57BL/6 mice and induction of apoptosis in the A549 cell line. Molecules, 2013; 18: 12916-12936.
Devkota KP, Khan MT, Ranjit R, Lannang AM, Samreen, Choudhary MI. Tyrosinase inhibitory and antileishmanial constituents from the rhizomes of Paris polyphylla. Nat Prod Res. 2007; 21: 321-327.
Zhang X, Cui Y, Huang J, Zhang Y, Nie Z, Wang L, Yan B, Tang Y, Liu Y. Immuno-stimulating properties of diosgenyl saponins isolated from Paris polyphylla. Bioorg. Med. Chem. Lett 2007; 17: 2408-2413.
Deng D, Lauren DR, Cooney JM, Jensen DJ, Wurms KV, Upritchard JE, Cannon RD, Wang MZ, Li MZ. Antifungal saponins from Paris polyphylla Smith. Planta Med. 2008; 24: 1397-1402.
Sun J, Liu BR, Hu WJ, Yu LX, Qian XP. In vitro anticancer activity of aqueous extracts and ethanol extracts of fifteen traditional Chinese medicines on human digestive tumor cell lines. Phytother Res. 2007: 21: 1102-1104.
He H, Sun YP, Zheng L, Yue ZG. Steroidal saponins from Paris polyphylla induce apoptotic cell death and autophagy in A549 human lung cancer cells. Asian Pac J Cancer Prev. 2015; 16: 1169-73.
Kang LP, Liu YX, Eichhorn T, Dapat E, Yu HS, Zhao Y, Xiong CQ, Liu C, Efferth T, Ma BP. Polyhydroxylated steroidal glycosides from Paris polyphylla. J Nat Prod. 2012; 75: 1201-1205.
Wen Y-S, Ni W, Qin X-J , Yan H, Chen C-X, Hua Y, Cheng Y-C, He L, Liu H-Y. Steroidal saponins with cytotoxic activity from the rhizomes of Paris polyphylla var. yunnanensis. Phytochem let. 2015; 12: 31-34.
Al Sawah E, Marchion DC, Xiong Y, Ramirez IJ, Abbasi F, Boac BM, Bush SH, Bou Zgheib N, McClung EC, Khulpateea BR, Berry A, Hakam A, Wenham RM, Lancaster JM, Judson PL. The Chinese herb polyphyllin D sensitizes ovarian cancer cells to cisplatin-induced growth arrest. J Cancer Res Clin Oncol. 2015; 141: 237-242.
Yang M, Zou J, Zhu H, Liu S, Wang H, Bai P, Xiao X. Paris saponin II inhibits human ovarian cancer cell-induced angiogenesis by modulating NF-κB signaling. Oncol Rep. 2015; 33: 2190-2198.
Kumar MH, Dhiman V, Choudhary R, Chikara A. Anticancer activity of hydroalcoholic Extracts from Paris polyphylla rhizomes against human A549 lung cancer cell lines using MTT assay. Int Res J pharmacy. 2014; 5: 290-294.