Preliminary phytochemical screening, analysis of phenolic compounds and antioxidant activity of Genista cephalantha Spach. (Fabaceae)

Authors

  • Chebbah Kaoutar Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physico-Chimiques et Biologiques (VARENBIOMOL), Université Constantine 1, Algérie.
  • Marchioni Eric Equipe de Chimie Analytique des Molécules Bioactives (IPHC-LC4, UMR 7178), Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin, 67400 Illkirch, France.
  • Menad Ahmed Laboratoire de Biologie et Environnement, Université Constantine 1, Route de Ain El Bey, 25 000 Constantine, Algérie.
  • Mekkiou Ratiba Department of Chemistry, Unit research: Valorisation des Ressources Naturelles et Analyses Physico-Chimiques et Biologiques, Université Constantine1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Sarri Djamel Département de Biologie, Faculté des Sciences, Université Mohammed Boudiaf, M’Sila, 28000, M’Sila, Algérie.
  • Ameddah Souad Laboratoire de Biologie et Environnement, Université Constantine 1, Route de Ain El Bey, 25 000 Constantine, Algérie.
  • Boumaza Ouahiba Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physico-Chimiques et Biologiques (VARENBIOMOL), Université Constantine 1, Algérie.
  • Seghiri Ramdane Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physico-Chimiques et Biologiques (VARENBIOMOL), Université Constantine 1, Algérie.
  • Benayache Samir Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physico-Chimiques et Biologiques (VARENBIOMOL), Université Constantine 1, Algérie.
  • Benayache Fadila Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physico-Chimiques et Biologiques (VARENBIOMOL), Université Constantine 1, Algérie

Keywords:

Phenolic compounds, Flavonoids, HPLC-DAD-UV, Antioxidant activity, Genista cephalantha, Fabaceae

Abstract

This study was designed to estabilish a preliminary phytochemical screening, evaluate the phenolic and flavonoid content according to the Folin-Ciocalteu procedure, and aluminum chloride method respectively and to determine qualitatively, using HPLC-UV method, the most important products present in ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts of the aerial parts of Genista cephalantha Spach. from east Algeria. The antioxidant activity of these extracts was spectrophotometrically tested by measuring their ability to scavenge a stable DPPH free radical and by β-Carotene/linoleic acid bleaching assay. Evaluated extracts showed a good activity in both antioxidant system assays.

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Published

30-09-2014

How to Cite

1.
Chebbah Kaoutar, Marchioni Eric, Menad Ahmed, Mekkiou Ratiba, Sarri Djamel, Ameddah Souad, Boumaza Ouahiba, Seghiri Ramdane, Benayache Samir, Benayache Fadila. Preliminary phytochemical screening, analysis of phenolic compounds and antioxidant activity of Genista cephalantha Spach. (Fabaceae). ijp [Internet]. 2014 Sep. 30 [cited 2024 Sep. 28];6(3):360-8. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/346

Issue

Vol. 6 No. 3 (2014): Jul- Sep

Section

Original Research Articles

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