Phytochemical screening, quantitative analysis and antioxidant activity of Lifago dielsii Schweinj. & Muschl. (Asteraceae)

Authors

  • Aïssaoui Hanane Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Département de Chimie, Faculté des Sciences Exactes, Université Constantine 1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Menad Ahmed Laboratoire de Biologie et Environnement, Faculté de Sciences de la Nature et de la vie, Université Constantine1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Mezhoud Samia Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Département de Chimie, Faculté des Sciences Exactes, Université Constantine 1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Ameddah Souad Laboratoire de Biologie et Environnement, Faculté de Sciences de la Nature et de la vie, Université Constantine1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Mekkiou Ratiba Department of Chemistry, Unit research: Valorisation des Ressources Naturelles et Analyses Physico-Chimiques et Biologiques, Université Constantine1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Benayache Samir Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Département de Chimie, Faculté des Sciences Exactes, Université Constantine 1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Benayache Fadila Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Département de Chimie, Faculté des Sciences Exactes, Université Constantine 1, Route de Aïn El Bey, 25 000 Constantine, Algeria.

Keywords:

Phytochemical screening, phenolic content, flavonoid content, antioxidant activity, Lifago dielsii, Asteraceae

Abstract

This study is designed to assess the phytochemical screening of Lifago dielsii Schweinj. & Muschl., endemic species localized in the South of Algeria, and to evaluate their potential antioxidant properties using 1,1-diphenyl-2-picrylhydrazyl (DPPH°) radical scavenging and lipid peroxidation inhibition (LPO; Fe2+/ascorbic acid system) assays. The phytochemical screening of the aerial parts of L. dielsii revealed the presence of triterpenoids, saponins, alkaloids, coumarins, flavonoids and tannins. Three fractions [chloroform (CHCl3), ethyl acetate (EtOAc), n-butanol (n-BuOH)] obtained from aqueous-MeOH extraction and the insoluble methanol (MeOH) part in water, were subjected to a quantitative determination of polyphenols and flavonoids. The antioxidant properties of all extracts were evaluated. The EtOAc fraction had the highest amount of total phenolic contents (TPC) compared to MeOH and n-BuOH) fractions whereas CHCl3 fraction showed the lowest level. The n-BuOH fraction was richer in total flavonoids content (TFC) (88.81%) compared to EtOAc (37.76%) and MeOH extract (37.88%). The CHCl3 fraction exhibited the weakest content of TFC (18.82%). The antioxidant activity revealed that the EtOAc extract seems to have the most powerful effect on the DPPH° scavenging effect (IC50=47.80 ± 2.20 µg/ml) and on LPO inhibition (IC50=113.24±0.65µg/ml). These results showed that L. dielsii would be suggested as a promising alternative source of the natural anti-oxidative phenolic compounds.

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Published

30-06-2014

How to Cite

1.
Aïssaoui Hanane, Menad Ahmed, Mezhoud Samia, Ameddah Souad, Mekkiou Ratiba, Benayache Samir, Benayache Fadila. Phytochemical screening, quantitative analysis and antioxidant activity of Lifago dielsii Schweinj. & Muschl. (Asteraceae). ijp [Internet]. 2014 Jun. 30 [cited 2024 Sep. 28];6(2):280-5. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/333

Issue

Vol. 6 No. 2 (2014): Apr-Jun

Section

Original Research Articles

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