Phytochemical screening, quantitative analysis and antioxidant activity of Lifago dielsii Schweinj. & Muschl. (Asteraceae)

Authors

  • Aïssaoui Hanane Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Département de Chimie, Faculté des Sciences Exactes, Université Constantine 1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Menad Ahmed Laboratoire de Biologie et Environnement, Faculté de Sciences de la Nature et de la vie, Université Constantine1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Mezhoud Samia Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Département de Chimie, Faculté des Sciences Exactes, Université Constantine 1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Ameddah Souad Laboratoire de Biologie et Environnement, Faculté de Sciences de la Nature et de la vie, Université Constantine1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Mekkiou Ratiba Department of Chemistry, Unit research: Valorisation des Ressources Naturelles et Analyses Physico-Chimiques et Biologiques, Université Constantine1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Benayache Samir Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Département de Chimie, Faculté des Sciences Exactes, Université Constantine 1, Route de Aïn El Bey, 25 000 Constantine, Algeria.
  • Benayache Fadila Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Département de Chimie, Faculté des Sciences Exactes, Université Constantine 1, Route de Aïn El Bey, 25 000 Constantine, Algeria.

Keywords:

Phytochemical screening, phenolic content, flavonoid content, antioxidant activity, Lifago dielsii, Asteraceae

Abstract

This study is designed to assess the phytochemical screening of Lifago dielsii Schweinj. & Muschl., endemic species localized in the South of Algeria, and to evaluate their potential antioxidant properties using 1,1-diphenyl-2-picrylhydrazyl (DPPH°) radical scavenging and lipid peroxidation inhibition (LPO; Fe2+/ascorbic acid system) assays. The phytochemical screening of the aerial parts of L. dielsii revealed the presence of triterpenoids, saponins, alkaloids, coumarins, flavonoids and tannins. Three fractions [chloroform (CHCl3), ethyl acetate (EtOAc), n-butanol (n-BuOH)] obtained from aqueous-MeOH extraction and the insoluble methanol (MeOH) part in water, were subjected to a quantitative determination of polyphenols and flavonoids. The antioxidant properties of all extracts were evaluated. The EtOAc fraction had the highest amount of total phenolic contents (TPC) compared to MeOH and n-BuOH) fractions whereas CHCl3 fraction showed the lowest level. The n-BuOH fraction was richer in total flavonoids content (TFC) (88.81%) compared to EtOAc (37.76%) and MeOH extract (37.88%). The CHCl3 fraction exhibited the weakest content of TFC (18.82%). The antioxidant activity revealed that the EtOAc extract seems to have the most powerful effect on the DPPH° scavenging effect (IC50=47.80 ± 2.20 µg/ml) and on LPO inhibition (IC50=113.24±0.65µg/ml). These results showed that L. dielsii would be suggested as a promising alternative source of the natural anti-oxidative phenolic compounds.

References

. Shams Ardekani MR, Hajimahmoodi M, Oveisi MR, Sadeghi N, Jannat B, Ranjbar AM, Gholam N, Moridi T. Comparative antioxidant activity and total flavonoid content of Persian pomegranate (Punicagranatum L.) cultivars. Iranian J. Pharm. Res.2011; 10(3): 519-24.

. Rice-Evans C, Miller N, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine. 1996 ; 20 : 933–956.

. Ozenda P. Flore du Sahara Septentrional et Central, Centre National de la Recherche Scientifique, Imprimerie Louis-Jean. H.A. 1958 ; pp432.

. Seghiri R, Boumaza O, Mekkiou R, Benayache S, Mosset P, Quintana J, Estévez F, León F, Bermejo J, Benayache F. A flavonoid with cytotoxic activity and other constituents from Centaurea africana. Phytochem. Lett. 2009 ; 2 : 114-118.

. Mezache N, Bendjedou D, Sata D, Mekkiou R, Benayache S, Benayache F. Secondary metabolites from Centaurea lippii (Asteraceae). Chem. Nat. Comp. 2010; 46: 801-802.

. Hammoud L, Seghiri R, Benayache S, Mosset P, Lobstein A, Chaabi M, León F, Brouard I, Bermejo J, Benayache F. A new flavonoid and other constituents from Centaurea nicaeensis All. var. walliana M. Nat. Prod. Res. 2012; 26: 203-208.

. Mezhoud S, Derbré S, Ameddah S, Mekkiou R, Boumaza O, Seghiri R, Benayache S, Richomme P, Benayache F. Antioxidant activity and chemical constituents of Warionia saharae Benth. & Coss. (Compositae) from Algeria. Int. J. Med. Arom. Plants. 2012 ; 2(3) : 509-513.

. Bicha S, Chalard P, Hammoud L, León F, Brouard I, Garcia VP, Lobstein A, Bentamene A, Benayache S, Bermejo J, Benayache F. Rec. Nat. Prod. 2013; 7(2) : 114-118

. Harborne JB. Phytochemical Methods. A guide to modern techniques of plant analysis, 3rd Edn. Chapman and Hall, London, 1998; 235.

. Khandelwal KR. Preliminary phytochemical screening, in Practical Pharmacognosy Techniques and Experiments. 8th edn. Nirali Publication, Pune, 2001; 149-156.

. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymol. 1999; 299:152–178.

. Ordon Ez AAL, Gomez JD, Vattuone MA, Isla MI. Antioxidant activities of Sechium edule (Jacq.) Swart extracts. Food Chem. 2006; 97: 452-458.

. Magalhães LM, Santos M, Segundo MA, Reis S, Lima JLFC. Automatic method for determination of total antioxidant capacity using 2;2-diphenyl-1- picrylhyrazyl assay. Anal. Chim. Acta. 2006; 558:310-318

. Aiyegoro OA, Okoh AI. Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complementary and Alternative Medicine. 2010; 10(21): 1-8.

. Adebayo AH, Tan NH, Akindahunsi AA, Zeng GZ, Zhang YM. Anticancer and antiradical scavenging activity of Ageratum conyzoides L. (Asteraceae). Pharmacognosy Magazine, 2010; 6(21): 62-66.

. Visintini Jaime MF, Redko F, Muschietti LV, Campos RH, Martino VS, Cavallaro LV. In vitro antiviral activity of plant extracts from Asteraceae medicinal plants. Virology Journal, 2013; 10: 245

. Mofor Teugwa1 C, Chouadeu Mejiato1 P, Zofou D, Tugnoua Tchinda B, Fekam Boyom F. Antioxidant and antidiabetic profiles of two African medicinal plants: Picralima nitida (Apocynaceae) and Sonchus oleraceus (Asteraceae). BMC Complementary and Alternative Medicine. 2013; 13: 175.

. Gupta A, Naraniwal M, Kothari V. Modern extraction methods for preparation of bioactive plant extracts. International Journal of Applied and Natural Sciences (IJANS). 2012; 1(1): 8-26.

. Elgabr MN, Mekkiou R, Ameddah S, Menad A, Boumaza O, Seghiri R, Benayache S, Benayache F. Antioxydant activities from the aerial parts of Pulicaria jaubertii. Advances in Natural and Applied Sciences. 2010; 4(1): 63-70.

. Öztürk N, Tunçel M, Potoğlu-Erkara İ. Phenolic compounds and antioxidant activities of

some Hypericum species: A comparative study with H. Perforatum. Pharmaceutical Biology. 2009; 47(2): 120–127.

. Ghasemzadeh A, Jaafar Hawa ZE, Rahmat A. Effects of solvent type on phenolics and flavonoids content and antioxidant activities in two varieties of young ginger (Zingiber officinale Roscoe) extracts. Journal of Medicinal Plants Research. 2011; 5(7): 1147-1154.

. Halliwell B, Guttridge J. The Free radicals in biology and medicine, Oxford University Press, Oxford. 1999.

. Gago-Dominguez M, Jiang X, Esteban CJ. Lipid peroxidation, oxidative stress genes and dietary factors in breast cancer protection: a hypothesis. Breast Cancer Research 2007; 9(201): 1-11.

. Min B, Ahn DU. Mechanism of Lipid Peroxidation in Meat and Meat Products -A Review Food Sci. Biotechnol. 2005; 14(1) : 152-163

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Published

30-06-2014

How to Cite

1.
Aïssaoui Hanane, Menad Ahmed, Mezhoud Samia, Ameddah Souad, Mekkiou Ratiba, Benayache Samir, Benayache Fadila. Phytochemical screening, quantitative analysis and antioxidant activity of Lifago dielsii Schweinj. & Muschl. (Asteraceae). ijp [Internet]. 2014 Jun. 30 [cited 2024 Dec. 22];6(2):280-5. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/333

Issue

Vol. 6 No. 2 (2014): Apr-Jun

Section

Original Research Articles

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