Antidiabetic effect of various fractions of Habenaria plantaginea root in streptozotocininduced diabetic rats
Keywords:
Habenaria plantaginea, antidiabetic, streptozotocinAbstract
The aim of this study is to evaluate the antidiabetic effect of methanolic extract of Habenaria plantaginea and its various fractions in different animal models. The effect of repeated oral administration of methanolic extract along with its n-hexane, ethyl acetate and n-butanol fractions on serum lipid profile and plasma enzyme levels in diabetic rats was also examined. The effect was found to be pronounced in n-butanol fraction. In oral glucose tolerance test, reduction of fasting blood glucose levels took place from 60 min on administration of methanolic extract and its various fractions. The n-butanol fraction has almost similar effect as that of standard drug Glibenclamide (10 mg/kg b. w). After 15 days of treatment, n-butanol fraction showed maximum reduction of blood glucose levels (64.28 %). Total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) levels were found to be decreased by 38.98, 28.06, 56.26 and 37.77% respectively in diabetic rats whereas, cardioprotective, high density lipoprotein (HDL) was increased by 31.65%. The n-butanol fraction also restored the altered plasma enzyme such as serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) and alkaline phosphatase (ALP) levels to near normal. These results clearly indicate that n-butanol fraction of methanolic extract of Habenaria plantaginea possess high antidiabetic potential along with significant hypoglycaemic and hypolipidemic effects.
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IAEC: Institutional Animal Ethical
Committee
OECD: Organization for Economic
Corporation and Development
ANOVA: Analysis of variance
i.p: Intraperitonial
min: Minutes
mg: Miligram
g: Gram
º C: Degree centigrade
h: Hour
OGTT: Oral glucose tolerance test
p.o: Per oral
STZ: Streptozotocin
WHO: WHO World health organization
TC: Total cholesterol
TG: Triglyceride
LDL: Low density lipoprotein
VLDL: Very low density lipoprotein
HDL: High density lipoprotein
SGOT: Serum glutamic oxaloacetic
transaminase
SGPT: Serum glutamic pyruvic
transaminase
ALP: Alkaline phosphatase
