Antidiabetic effect of various fractions of Habenaria plantaginea root in streptozotocininduced diabetic rats

Authors

  • Goutam Ghosh School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Bhubaneswar-751003, Odisha, India
  • Debajyoti Das School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Bhubaneswar-751003, Odisha, India
  • Agnimitra Dinda School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Bhubaneswar-751003, Odisha, India
  • Paidisethy Sudhir Kumar School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Kalinga Nagar, Bhubaneswar-751003, Odisha, India

Keywords:

Habenaria plantaginea, antidiabetic, streptozotocin

Abstract

The aim of this study is to evaluate the antidiabetic effect of methanolic extract of Habenaria plantaginea and its various fractions in different animal models. The effect of repeated oral administration of methanolic extract along with its n-hexane, ethyl acetate and n-butanol fractions on serum lipid profile and plasma enzyme levels in diabetic rats was also examined. The effect was found to be pronounced in n-butanol fraction. In oral glucose tolerance test, reduction of fasting blood glucose levels took place from 60 min on administration of methanolic extract and its various fractions. The n-butanol fraction has almost similar effect as that of standard drug Glibenclamide (10 mg/kg b. w). After 15 days of treatment, n-butanol fraction showed maximum reduction of blood glucose levels (64.28 %). Total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) levels were found to be decreased by 38.98, 28.06, 56.26 and 37.77% respectively in diabetic rats whereas, cardioprotective, high density lipoprotein (HDL) was increased by 31.65%. The n-butanol fraction also restored the altered plasma enzyme such as serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) and alkaline phosphatase (ALP) levels to near normal. These results clearly indicate that n-butanol fraction of methanolic extract of Habenaria plantaginea possess high antidiabetic potential along with significant hypoglycaemic and hypolipidemic effects.

References

. Chandra A, Singh RK, Tewari L.

Antioxidative potential of herbal

hypoglycemic agents in diabetes-an

overview. SFRR-Indian Bulletin

;3:24–26.

. Kesari AN, Kesar S, Singh SK, Gupta

RK, Watal G. Studies on the glycemic

and lipidemic effect of Murraya koenigii

in experimental animals. J

Ethnopharmacol. 2007;112:305–311.

. Day C. Traditional plant treatment for

diabetes mellitus: pharmaceutical foods.

Britain J Nutri. 1998;80:5–6.

. Boyle JP, Honneycutt AA, Narayan KM,

Hoerger TJ, Geiss LS, Chen H,

Thompson TJ. Projections of diabetes

burden through 2050: impact of

changing demography and disease

prevalence in the US. Diabetes Care

;24:1936–1940.

. Zhang BB, Moller DE. News approaches

in the treatment of type 2 diabetes.

Current Opinion of Chemical Biology

;4:461–467.

. Dhanbal SP. Evaluation of therapeutic

activity and development of quality

control profiles for some antidiabetic

herbal drugs. Indian J Pharm Educ.

;8:163–165.

. Nadkarni KM, Nadkarni AK. The Indian

Materia Medica. Mumbai, India: Popular

Prakashan (Pvt.) Ltd.; 1996;p. 964-

:(vol 1)

. Maridass M, Zahir Hussain MI, Raju G.

Phytochemical Survey of Orchids in the

Tirunelveli Hills of South India.

Ethnobotanical Leaflets 2008;12:705-

. Mohammad HM. Therapeutic Orchids:

Traditional uses and recent advances –

An Overview. Fitoterapia 2011;82:102-

. Harborne JB. Phytochemical Methods.

London: Chapman & Hall; 1998;p.60.

. Ghosh MN. Fundamental of

Experimental Pharmacology. 3rd ed.

Kolkata: Hilton and Company;

;p.197.

. Theodorou NA, Vrbova H, Tyhurst M,

Howell SL. Management of intestinal

amoebiasis by an indigenous drug

Kantaki karanja (Caesalpinia crista L.).

Diabetol. 1980;18:313-318.

. Andallu B, Varadacharyulu NC. Control

of hyperglycemia and retardation of

cataract by mulberry (Morus indica L.)

leaves in streptozotocin diabetic rats.

Indian J Exp Biol. 2002;40:791-795.

. Kesari AN, Gupta RK, Singh SK,

Diwakar S, Watal G. Hypoglycemic and

antihyperglycemic activity of Aegle

marmelos seed extract in normal and

diabetic rats. J Ethnopharmacol.

;107:374–379.

. Shirwaikar A, Rajendran K, Barik, R.

Effect of aqueous bark extract of Garuga

pinnata Roxb. in streptozotocin–

nicotinamide induced type-II diabetes

mellitus. J Ethnopharmacol.

;107:285–290.

. Burkhardt RT, Batsakis JC. An

interlaboratory comparison of serum

total protein analysis. Am J Clin Pathol.

;70:508–510.

. Pierre NM, Demaekerl A, Marja H, Helga

TD, Henk B. Precipitation methods for

high density lipo-protein cholesterol

measurement compared, and final

evaluation under routine operating

conditions of a method with a low

sample-to-reagent ratio. Clin Chem.

;43:663–668.

. MeGown MW, Artiss JD, Strandberg

DR, Zak B. A peroxidase-coupled

method for the colorimetric

determination of serum triglycerides.

Clin Chem. 1983;29:538–542.

. Kind PRN, King EJ. Estimation of

plasma phosphatase by determination of

hydrolysed phenol with antipyrin. J Clin

Pathol.1954;7:322-331.

. Bailey CJ, Day C. Traditional plant

medicines as treatments for diabetes.

Diabetes Care 1989;12:553-564.

. Trivedi NA, Mazumdar B, Bhatt JD,

Hemavathi KG. Effect of Shilajit on blood

glucose and lipid profile in alloxan

induced diabetic rats. Indian J

Pharmacol. 2004;36:373-376.

. Hardy KJ, McNutty SJ. Oral

hypoglycemic agents. Med Digest

;23:5-9.

. Badole S, Patel N, Bodhankar S, Jain B,

Bhardwaj S. Antihyperglycemic activity

of aqueous extract of leaves of Cocculus

hirsutus (L.) Diels in alloxan-induced

diabetic mice. Indian J Pharmacol.

;38:49-53.

. Jacot E, Assal JPH. Regulation de la

glycemie. In: Pharmacologiedes

Concepts Fondamentaux aux

Applications Therapeutiques, editor

Schorderet. 1989;p. 481-494.

. Marles RJ, Farnsworth NR. Antidiabetic

plants and their active constituents.

Phytomedicine 1995;2:137–189.

. Ali L, Azad Khan AK, Mamun MIR,

Mosihuzzaman M, Nahar N, Alan MNE,

Rokeya B. Studies on the hypoglycemic

effects of fruits pulp, seed and whole

plant of Momordica charantia on

normaland diabetic model rats. Planta

Med. 1993;59:408–412.

. Kamanyi A, Dajmen D, Nkeh B.

Hypoglycemic properties of the aqueous

root extract of Morinda lucida (Rubiacea)

study in the mouse. Phytother Res.

;8:369–371.

. Foreston WC, Tedesco FJ, Starnes EC.

Marked elevation of serum transaminase

activity associated with extrahepatic

biliary tract disease. J Clin

Gastroenterol. 1985; 76:502–505.

. Hultcrantz R, Glaumann H, Lindberg G.

Liver investigation in 149 asymptomatic

patients with moderately elevated

activities of serum aminotransferases. J

Gastroenterol. 1996;21:109–113.

. Ohaeri OC. Effect of garlic oil on the

levels of various enzymes in the serum

and tissue of streptozotocin diabetic

rats. Bio sci Rep. 2001;21:19–24.

. Navarro CM, Montilla PM, Martin A,

Jimenez J, Utrilla PM. Free radicals

scavenger and antihepatotoxic activity of

Rosmarinus. Planta Med. 1993;59:312–

. Almdal TP, Vilstrup H. Strict insulin

treatment normalizes the organic

nitrogen contents and the capacity of

urea–N synthesis in experimental

diabetes in rats. Diabetologia

;31:114–118.

. Taskinen MR. Lipoprotein and

apoproteins in diabetes, in Current

Topics in Diabetes Research, editors.

Belfiore F, Bergnan RN, Molinatt GM,

Informa Health Care. 1993;p. 122–134.

. Wilson PWF. High density lipoprotein,

low density lipoprotein and coronary

heart disease. American J Cardiol.

;66:7A–10A.AbbreviationDM:

Diabetes mellitus

IAEC: Institutional Animal Ethical

Committee

OECD: Organization for Economic

Corporation and Development

ANOVA: Analysis of variance

i.p: Intraperitonial

min: Minutes

mg: Miligram

g: Gram

º C: Degree centigrade

h: Hour

OGTT: Oral glucose tolerance test

p.o: Per oral

STZ: Streptozotocin

WHO: WHO World health organization

TC: Total cholesterol

TG: Triglyceride

LDL: Low density lipoprotein

VLDL: Very low density lipoprotein

HDL: High density lipoprotein

SGOT: Serum glutamic oxaloacetic

transaminase

SGPT: Serum glutamic pyruvic

transaminase

ALP: Alkaline phosphatase

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Published

31-03-2012

How to Cite

1.
Goutam Ghosh, Debajyoti Das, Agnimitra Dinda, Paidisethy Sudhir Kumar. Antidiabetic effect of various fractions of Habenaria plantaginea root in streptozotocininduced diabetic rats. ijp [Internet]. 2012 Mar. 31 [cited 2024 Dec. 23];4(1):90-8. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/149

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