Antioxidant, haemolytic activities and GC-MS profiling of Carissa carandas roots.

Authors

  • Farah Aslam Department of Chemistry, Government College University Faisalabad-38400, Pakistan
  • Nasir Rasool Department of Chemistry, Government College University Faisalabad-38400, Pakistan
  • Muhammad Riaz Department of Chemistry, Government College University Faisalabad-38400, Pakistan
  • Muhammad Zubair Department of Chemistry, Government College University Faisalabad-38400, Pakistan
  • Komal Rizwan Department of Chemistry, Government College University Faisalabad-38400, Pakistan
  • Mazhar Abbas Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad- 8040, Pakistan
  • Tanveer Hussain Bukhari Department of Chemistry, Government College University Faisalabad-38400, Pakistan
  • Iftikhar Hussain Bukhari Department of Chemistry, Government College University Faisalabad-38400, Pakistan

Keywords:

Antioxidants, corn oil, Carissa carandas, roots, lipids

Abstract

The antioxidant potential of the plant Carissa carandas roots extracted with different polarity based solvents was assessed. The antioxidant activity was evaluated by the measurement of total phenolic contents (TPC), total flavonoid contents (TFC), reducing power, DPPH radical scavenging, IC50 and antioxidant activity in linoleic acid system. The plant material contained the TPC (1.79-4.35 GAE mg/g of dry extract), TFC (1.91-3.76 CE mg/g of dry extract), DPPH radical scavenging activity, IC50 (12.53-84.82 %) and % inhibition of peroxidation in linoleic acid (41.0-89.21 %) system respectively. Furthermore the antioxidant effectiveness of extracts was assessed using corn oil (CO) as the oxidation substrate. The oxidative alterations were evaluated by the measurement of conjugated diene (CD), conjugated triene (CT), panisidine, free fatty acid (FFA) and peroxide values (PV). The cytotoxicity of the plant extracts were assessed against human red blood cells (RBCs) and the % lysis was found to be in the range of 1.01-6.10 %. The GC-MS analysis of n-hexane extract was also profiled. It was concluded that extracts of plant roots may be used as potential source of antioxidant agents in food industries.

References

Khare CP. Indian Medicinal Plants (an

Illustrated Dictionary), Springer Science,

New York, USA; 2007. 123,472.

Sathish CJ, Sharma RA, Jain R, Macalo N,

Capasso F, Vijayvergia R and Mittal C.

Ethno pharmacological evaluation of

Pergularia daemia (Forsk.) Chivo.

Phytotherapy Res. 1998; 12:378-380.

Anjaneyulu ASN, Raju DVSN, Srinivasa

Rao S. chemical evaluation of pergularia

daemia. Indian J. of Chem. 1998;

B:318-320.

Bhaskar VH, Balakrishnan N.

Hepatoprotective activity of laticiferous

plant species Pergularia daemia and

Carissa Carandas from Western Ghats,

Tamilnadu, India. An International Peer

Reviewed. J. of Pharm. Sci. 2009; 1(2):

-142.

Chatterjee ML, Roy AR. Pharmacological

action of Carissa carandas root. Bulletin

of the Calcutta School of Tropical Medi.

; 13(1):14-16.

Joglekar, SN, Gaitonde, BB. Histamine

releasing activity of Carissa Carandas

roots (Apocynaceae). Japanese of J. of

Pharmacol. 1970; 20:367.

Zaki A, Tohamy S, Fattah S. Study of

Lipid content and volatile oil of the

different organs of Carissa Carnadas Linn.

And Carissa grandiflora Dc. growing in

Egypt. Egyptian J. of Pharma. Sci. 1983;

(1-4):127-141.

Sharma alok, Reddy GD, Kaushik Atul K,

Shankar, Tiwri RK, Alok Mukherjee, Rao

ChV. Nat. Prod. Sci. 2007; 13(1): 6-10.

Mala V, Dahot MU. Lipase activity of

Carissa carandas fruits. Science

International-Lahore. 1995; 7(2): 161-164.

Chandra G. Essential Oil of Carissa

carandas. Examination of the benzene

extract of the flowers and of the essential

oil. Soap, Perfum. and Cosmet. 1972; 45

(6): 551-556.

Naim Z, Khan M, Nizami S. Isolation of a

new triterpenic alcohol from of Carissa

carandas. Pakistan J. of Sci. and Indus.

Res. 1985; 28(6): 378-381.

Pal R, kulshreshtha DK, Rastogi RP. A

new lignan from Carissa carandas.

Phytochemistry 1975; 14(10): 2302-2303.

Pino J , Marbot R, Vazques C. Volatile

flavor constituents of Karanda (Carissa

carandas L.) fruit. J. of Esse. Oil Res.

; 16(5): 432-434.

Siddiqui S, Ghani U, Ali S, Usmani S,

Begum S. Triterpenoidal Constituents of

the leaves of Carissa carandas. Nat. Prod.

Sci. 2003; 31(11):753-755.

Sarin Y, Kapoor L. Vegetable tannin

recourses of Jammu and Kashmir. Bulletin

of the Reg. Res. Lab. – Jammu. 1963;

:136-141.

Naim Z, Khan M, Nizami S. Isolation of a

new isomer of ursolic acid from fruits and

leaves of Carissa carandas. Pakistan J. of

Sci. and Indus. Res. 1988; 31(11): 753-

Edeoga HO, Okwu DE, Mbaebie BO.

Phytochemical constituents of some

Nigerian medicinal plants. African J. of

Biotec. 2005; 4(7): 685-688.

Chaovanalikit A and Wrolstad RE. Total

anthocyanins and total phenolics of fresh and

processed cherries and their antioxidant

properties. Food Chem. And Toxicol. 2004;

:67-72.

Dewanto V, Wu X, Adom KK and Liu RH.

Thermal processing enhances the nutritional

value of tomatoes by increasing total

antioxidant activity. J. of Agri. and Food

Chem. 2002; 50:3010–3014.

Bozin B, Mimica-Dukie N, Simin N, and

Anackov G. Characterization of the volatile

composition of essential oil of some

lamiaceae species and the antimicrobial and

antioxidant activities of the entire oils. J. of

Agri. and Food Chem. 2006; 54:1822-1828.

Iqbal S, Bhanger MI and Anwar F.

Antioxidant properties and components of

some commercially available varieties of

rice bran in Pakistan. Food Chem.2007;

:265-272.

Yen GC, Duh PD and Chuang YD.

Antioxidant activity of anthraquinones and

anthrone. Food Chem. 2000; 70:307–315.

Powell WA, Catranis CM, Maynard CA.

Design of self processing antimicrobial

peptides for plant protection. Letters in

Applied Micro. 2000; 31:163-168.

Anwar F, Bhanger MI Iqbal S. Long term

antioxidant activity guided evaluation of

Soft fruits and extracts in Synthetic

antioxidant in Soybean oil J. of Japan Oil

Chemist Soc. 2003; 12(2): 18-20.

American Oil Chemists Society. Official

and Recommended Practices of the

American Oil Chemists Society, 5th ed.,

USA: AOCS Press Champaign IL. 1997.

Sultana B, Anwar F, Asi MR, Chatha SA.

Antioxidant potential of extract from

different agro wastes: stabilization of corn

oil. Grasas y aceites. 2008; 59(3):205-217.

International Union of Pure and Applied

Chemistry (IUPAC). Standard Methods for

the Analysis of Oils, Fats and Derivatives,

th Rev. edited by C. Paquot and A.

Hautfenne, Blackwell Scientific, London.

Orishadipe AT, Okogun JI, Mishelia E.

Gass chromatography-mass spectrometry

analysis of the hexane extract of

Calliandra portoricensis and

itsantimicrobial activity. African J. of Pure

and Applied chem. 2010; 4:131-134.

Nazifi E, Delazar A, Movafeghi A,

Hemmati S, Nazemiyeh H, Nahar L,

Sarker SD. GC-MS of the dichloromethane

extract of the bulbs of Ornihogalum

cuspidatum Bert. (Family: Liliaceae) from

Iran. Record of Nat. Prod. 2008; 2:94-99.

Hedge K, Thakker SP, Joshi AB, Shastry

CS, Chandrashekhar KS. Anticonvulsant

activity of Carissa Carandas Linn. Root

extract in experimental mice. Tropical J. of

pharma. Res. 2009; 8(2):117-125.

Bhaskar VH, Balakrishnan N. Analgesic,

anti-inflamatory and antipyretic activities

of Pergularia deamia and Carissa

Carandas. Daru. 2009; 17(3):168-174.

Downloads

Published

31-12-2011

How to Cite

1.
Farah Aslam, Nasir Rasool, Muhammad Riaz, Muhammad Zubair, Komal Rizwan, Mazhar Abbas, Tanveer Hussain Bukhari, Iftikhar Hussain Bukhari. Antioxidant, haemolytic activities and GC-MS profiling of Carissa carandas roots. ijp [Internet]. 2011 Dec. 31 [cited 2024 Feb. 29];3(4):567-78. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/138

Issue

Section

Original Research Articles

Most read articles by the same author(s)