Identification of β-carotene and β-sitosterol in methanolic extract of Dipteracanthus patulus (Jacq) nees and their role in antimicrobial and antioxidant activity

Authors

  • Shrinivas B Bumrela Sinhgad Technical Education Society’s, Sinhgad Institute of Pharmaceutical Sciences, Off Mumbai Pune. Expressway, Kusgaon (Bk), Lonavala, Dist-Pune, Pin: 410401, MS, India.
  • Suresh R Naik Sinhgad Technical Education Society’s, Sinhgad Institute of Pharmaceutical Sciences, Off Mumbai Pune. Expressway, Kusgaon (Bk), Lonavala, Dist-Pune, Pin: 410401, MS, India.

Keywords:

Dipteracanthus patulus, HPTLC, β-carotene, β-sitosterol, iridoid glycosides, Antioxidant activity, Antimicrobial activity

Abstract

The Dipteracanthus patulus (Jacq) nees is undershrub belonging to the family acantheaceae. Antimicrobial activity (disc diffusion method) and antioxidant activity by different in-vitro methods (DPPH, hydrogen peroxide, nitric oxide radical scavenging and reducing power) of methanolic extract of Dipteracanthus patulus (MEDP) was evaluated. The qualitative and quantitative estimation of β-carotene and β- sitosterol in MEDP was carried out by high performance thin layer chromatography (HPTLC). The total phenolic content of was determined by Folin-Ciocalteu method. Experimental findings indicate promising antimicrobial activity (antibacterial and antifungal) and potent antioxidant activity of MEDP. In addition, phytochemical analysis and spectral studies of MEDP were also performed. It is presumed that antimicrobial and antioxidant activity observed with MEDP may largely be attributed to the presence of major phytoconstituents (β-carotene, β-sitosterol and iridoid glycosides) and other minor components may participate as promoters.

References

Chirangini P, Amit K, Pandey BN,

Sharma GJ, Mishra KP. Herbals for

human health and disease: prospects and

challenges in 21st century. In: Sharma RK,

Arora R, editors. Herbal Drugs - A

Twenty First Century Perspective. New

Delhi: Jaypee Brothers Medical

Publishers; 2006;p. 1-7.

Piddock LJV, Wise R. Mechanisms of

resistance to quinolones and clinical

perspectives. J. Antimicro. Chemother.

;23(4),475-480.

Parida MM, Dash PK, Saxena P, Jana

AM, Rao PVL. Perspectives on

antimicrobial activity of natural plant

products. In: Sharma RK, Arora R,

editors. Herbal Drugs - A Twenty First

Century Perspective. New Delhi: Jaypee

Brothers Medical Publishers; 2006;p.

-496.

Narasimhan S, Shobana R, Sathya TN.

Antioxidants- natural rejuvenators that

heal, detoxify and provide nourishment.

In: Sharma RK, Arora R, editors. Herbal

Drugs - A Twenty First Century

Perspective. New Delhi: Jaypee Brothers

Medical Publishers; 2006;p.548-558.

Chopra RN, Nayar SL, Chopra IC.

Glossary of Indian Medicinal Plants. New

Delhi: Council of Scientific and Industrial

Research. 1986;p.99.

Akthar MF. Chemical and biological

investigations of medicinal herbs phyla

nodiflora, ruellia patula and ruellia

brittoniana. Dissertation submitted to

University of Karachi, Department of

Pharmacognosy, Faculty of Pharmacy,

Pakistan. 1993;p. 59.

Manikandan A, Doss DVA. Evaluation of

biochemical contents, nutritional value,

trace elements, SDS-PAGE and HPTLC

profiling in the leaves of Ruellia tuberosa

L. and Dipteracanthus patulus (Jacq.). J.

Chem. Pharm. Res. 2010;2(3):295-303.

Saroja K, Elizabeth JD, Gopalkrishnan S.

Wound healing activity of leaves of

Dipteracanthus patulus (Jacq.).

Pharmacologyonline. 2009;2:462-469.

Manikandan A, Doss DVA. Effect of 50%

hydroethanolic leaf extracts of Ruellia

tuberosa L. and Dipteracanthus patulus

(Jacq.) on AST, ALT, ACP and ALP

levels in serum, liver and kidney of

alloxan induced diabetic rats. Anna.

Pharm. Pharm. Sci. 2010;1(2):142-146.

Akhtar MF, Rashid S, Ahmad M,

Usmanghani K. Cardiovascular evaluation

of Ruellia patula and Ruellia brittoniana.

J. Islamic. Acad. Sci. 1992;5(1):67-71.

Ansari SH. Essentials of Pharmacognosy.

Delhi: Birla Publication; 2006;p.357-383.

Harborne JB. Phytochemical methods: A

guide to modern techniques of plant

analysis. London: Chapman and Hall

Publication; 1998;p. 119.

Slinkard K, Singleton VL. Total phenol

analysis: automation and comparison with

manual methods. Am. J. Enol. Viticult.

;28:49–55.

Chen Y, Wang MF, Rosen RT, Ho CT. 2,

-Diphenyl -1 -picrylhydrazyl radicalscavenging active components from

Polygonum multiflorum Thunb. J. Agric.

Food Chem. 1999;47:2226–2228.

Green LC, Wagner DA, Glogowiski J,

Skipper PL, Wishnok JS, Tannenbaum

SR. Analysis of nitrate, nitrite and 15N

nitrate in biological fluids. Anal.

Biochem. 1982;126:131-138.

Ruch RJ, Cheng SJ, Klaunig JE.

Prevention of cytotoxicity and inhibition

of intercellular communication by

antioxidant catechins isolated from

Chinese green tea. Carcinogenesis.

;10:1003–1008.

Oyaizu M. Studies on products of

browning reactions: antioxidative

activities of products of browning reaction

prepared from glucosamine. Jpn. J. Nutr.

;44:307–315.

Benzie IFF, Strain JJ. The ferric reducing

ability of plasma (FRAP) as a measure of

antioxidant power: The FRAP assay.

Anal. Biochem. 1996;239:70–76.

Wong SP, Leong LP, Koh JHW.

Antioxidant activities of aqueous extracts

of selected plants. Food Chem.

;99:775-783.

Kirby MDK, Bauer RW, Sherris JC,

Turck M. Antibiotic susceptibility testing

by standard single disc diffusion method.

Am. J. Clin. Pathol. 1966;45:493-496.

Godinho A, Bhosale S. Carotenes

produced by alkaliphilic orangepigmented strain of microbacterium

arborescens-AGSB isolated from coastal

sand dunes. Indian J. Mar. Sci.

;37(3):307-312.

Yamaguchi T, Takamura H, Matoba T,

Terao J. HPLC method for evaluation of

the free radical-scavenging activity of

foods by using 1,1,-diphenyl-2-

picrylhydrazyl. Biosci. Biotech. Biochem.

;62:1201–1204.

Chen CW, Ho CT. Antioxidant properties

of polyphenols extracted from green and

black tea. J. Food Lipids. 1995;2:35–46.

Bors W, Heller W, Michael C, Saran M.

Radical chemistry of flavonoids

antioxidants. Adv. Exp. Med. Biol.

;264:165–170.

Djeridane A, Yousfi M, Nadjemi B,

Boutassouna D, Stocker P, Vidal N.

Antioxidant activity of some Algerian

medicinal plants extracts containing

phenolic compounds. Food Chem.

;97:654–660.

Senthilkumar N, Badami S, Dongre SH,

Bhojraj S. Antioxidant and

hepatoprotective activity of methanolic

extract of Careya arborea bark in Ehrlich

ascites carcinoma bearing mice. J. Nat.

Remedies. 2008;62:336-339.

Da Silva VS, Silvab GH, Bolzani VDS,

Lopes MN. Isolation of lignin glycoside

from Alibertia sessilis (Vell) K. Schum

(Rubiaceae) by preparative HPLC.

Ecletica Quimica 2006;31(4):55-58.

Sies H, Stahl W. Vitamin E and C, beta

carotene and other carotenoids as

antioxidants. Am. J. Clin. Nutr.

;62:15S-21S.

Scalbert A. Antimicrobial properties of

tannins. Phytochemistry. 1991;30:3875-

Hagerman AE, Butler LG. The specificity

of proanthocyanidin-protein interactions.

The J. Biol. Chem. 1981;256:4494–4497.

Haslam E. Natural polyphenols (vegetable

tannins) as drugs: possible modes of

action. J. Nat. Prod. 1996;59:205–215.

Beltrame FL, Pessini GL, Doro DL, Filho

BPD, Bazotte RB, Cortez DAG.

Evaluation of the antidiabetic and

antibacterial activity of Cissus sicyoides.

Braz. Arch. Biol. Tech. 2002;45(1):21-

Akunyili DN, Houghton PJ, Raman A.

Antimicrobial activities of the stembark of

Kigelia pinnata. J. Ethonopharmacol.

;35(2):173-177.

Lee DG, Jung HJ, Woo ER. Antimicrobial

property of (+)-lyoniresinol-3alpha-Obeta-D-glucopyranoside isolated from the

root bark of Lycium chinense Miller

against human pathogenic

microorganisms. Arch. Pharm. Res.

;28(9):1031-1036.

Downloads

Published

30-06-2011

How to Cite

1.
Shrinivas B Bumrela, Suresh R Naik. Identification of β-carotene and β-sitosterol in methanolic extract of Dipteracanthus patulus (Jacq) nees and their role in antimicrobial and antioxidant activity. ijp [Internet]. 2011 Jun. 30 [cited 2024 Dec. 6];3(2):204-15. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/98

Issue

Section

Original Research Articles