Constituents of the rhizome of Curcuma aeruginosa and its DNA fingerprint

Authors

  • Chantana Aromdee Associate Professor, Faculty of Pharmaceutical Sciences, Khon Kaen. University, 40002, Thailand.
  • Senee Polrat Graduate School, Khon Kaen University, 40002, Thailand.
  • Preeya Wangsomnuek Department of Biology, Faculty of Sciences, Khon Kaen University, 40002, Thailand.

Keywords:

Curcuma aeruginosa, Zingiberaceae, TLC, GC-MS, DNA fingerprints

Abstract

Identity of the rhizhome of Curcuma aeruginosa Roxb. was established by three techniques: (1) the DNA fingerprint, (2) the chemical constituents of its volatile oils by using gas chromatographmass spectrometer, and (3) thin-layer chromatography (TLC) of the methanol extract. These three techniques were used to differentiate C. aeruginosa from its similar species. Result from the polymerase chain reaction (PCR) amplification, different polymorphic bands between the two specimens were found. The relative amounts of camphor, curzerenone and epicurzerenone in the C. aeruginosa rhizome were 16.85, 16.81 and 3.5% of total peak areas, whereas 6.04, 0 and 62.84% of total peak areas were found in the Curcuma sp. The thin-layer chromatogram revealed that Curcuma sp. contained curcumine, whereas only traces were detected in C. aeruginosa.

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Published

30-06-2011

How to Cite

1.
Chantana Aromdee, Senee Polrat, Preeya Wangsomnuek. Constituents of the rhizome of Curcuma aeruginosa and its DNA fingerprint. ijp [Internet]. 2011 Jun. 30 [cited 2024 Dec. 6];3(2):198-203. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/97

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Section

Original Research Articles