Rapid RP-HPLC technique for the determination of phyllanthin as bulk and its quantification in Phyllanthus amarus extract

Authors

  • Amene Alvari Centre for Transgenic Plant Development, Dept. Biotechnology, F/OScience, Jamia Hamdard, New Delhi-62, India
  • Mehrnaz S. Ohadi Rafsanjani Centre for Transgenic Plant Development, Dept. Biotechnology, F/OScience, Jamia Hamdard, New Delhi-62, India
  • Farhan J. Ahmed Dept. Pharmaceutics, F/O- Pharmacy, Jamia Hamdard, New Delhi- 110062, India
  • Mohd S. Hejazi Dept. Pharmacy, Tabriz University, Tabriz, Iran.
  • Malik Z. Abdin Centre for Transgenic Plant Development, Dept. Biotechnology, F/OScience, Jamia Hamdard, New Delhi-62, India

Keywords:

Phyllanthus amarus, Phyllanthin, HPLC and Plant extract

Abstract

A simple, specific and precise high performance liquid chromatographic method has been developed and validated for the estimation of Phyllanthin, an important bitter lignan present in Phyllanthus amarus. Furthermore, the developed method was used to successfully quantify the Phyllanthin in the plant extract also. The mobile phase optimized for the RP-HPLC was ethanol-water 66:34 (%v/v) and that was very simple and cost effective. The detection was carried out using variable wavelength UV–VIS detector set at 229 nm. Linearity for the developed method was found over the concentration range 1–50 μg/ml with a correlation coefficient of 0.999. Method was validated according to ICH guidelines and found to be suitable for rapid quantification of Phyllanthin in bulk and in plant extracts.

References

Thagarajan SP, Thiruneelakantan K,

Subramaniam S, Sundaravelu T. Invitro

inactivation of HBsAg by Eclipta alba and

Phyllanthus niruri Linn. Ind. J. Med. Res.

;76:124-130.

Thagarajan SP, Subramaniam S,

Thirunalasundari T. Effect of Phyllanthusamarus on chronic carriers of Hepatitis B

virus. The Lancet. 1988;11:764-766.

Venkateswaran PS, Millman I, Blumberg

BS. Effect of an extract from Phyllanthus

niruri on Hepatitis B and Woodchuck

Hepatitis viruses: Invitro and invivo

studies, Proc. Natl. Acad. Sci. USA.

;84:274-278.

Mehrotra R, Rawat S, Kulshreshtha DK.

Invitro effect of Phyllanthus amarus on

Hepatitis B virus. Ind. J Med. Res.

;93:71-73.

Jayaram S, Thyagarajan SP. Inhibition of

HBsAg secrection from Alexander cell

line by Phyllanthus amarus. Indian

J.Pathol. Microbiol. 1996;39:211-215.

Yeh SF, Hong CY, Huang YL, Liu TY,

Choo KB, Chou CK. Effect of an extract

from Phyllanthus amarus on Hepatitis B

surface antigen gene expression in human

hepatoma cells. Antiviral Res.

;20:185-192.

Lee CD, Ott M, Thayagarajan SP, Shafritz

DA, Burk RD, Gupta S. Phyllanthus

amarus down regulates Hepatitis B virus

mRNA transcription and replication. Eur.

J. Clin. Invest. 1996;26:1069-1076.

Ott M, Thyagarajan SP, Gupta S.

Phyllanthus amarus suppresses Hepatitis

B virus by interrupting interaction

between HBV enhancer I and cellular

transcription factors. Eur. J. Clin. Invest.

;27:908-915.

Ramakrishnan PN, Murugesan R,

Palanichamy S, Murugesh N. Oral

hypoglycaemic effect of Phyllanthus

niruri Linn. Leaves. Ind.J.Pharmaceu. Sci.

;44:10-12.

Moshi MJ, Uiso FC, Mahunnah R.L. A

study of the effect of Phyllanthus amarus

extract on blood glucose in rabbits. Int J.

Pharmacog. 1997;35:167-173.

Srividya N, Periwal S. Diuretic,

hypotensive and hypoglycaemic effect of

Phyllanthus amarus. Ind. J. Exp. Biol.

;33:861-864.

Sivaprakasam K, Yasodha R, Sivanandam

G, Veluchamy G. Clinical evaluation of

Phyllanthus amarus Schum & Thonn. in

Diabetes Mellitus. Seminar on research in

Ayurveda and Siddha, CCRAS, New

Delhi. 1995;20-22: p17.

Tripathi SC, Patnaik GK, Visen PKS.

Evaluation of hepatoprotective activity of

Phyllanthus amarus against

experimentally induced liver damage in

rats. Proc. 25th Ind. Pharmacol. Soc.

Conf., Muzaffarpur, Bihar, India.

;December 5th - 8th:82.

Sane RT, Kuber VV, Chalissery MS.,

Menon S. Hepatoprotection by

Phyllanthus amarus and Phyllanthus

debili in CCL4 induced liver dysfunction.

Current Science. 1995;68:1243-1246.

Venkateswaran PS, Millman I, Blumberg

BS. Effects of an extract from Phyllanthus

niruri on Hepatitis B and Woodchuck

hepatitis viruses Invitro and Invivo

studies Proc. Natl. Acad. Sci. USA.

;84:274-278.

Calixto JB, Santos AR, Cechinel Filho V,

Yunes RA. A review of the plants of the

genus Phyllanthus: their chemistry.

Pharmacology and therapeutic potential.

Med Res Rev. 1998;18:225-258.

Shakila A, Rajeswari R. Effect ofGA3,

certain chemicals and media on

germination, growth and yield of

Phyllanthus niruri. Plant Arch.

;6:121–125.

ICH Haromonised Tripartite Guideline,

International Conference on

Harmonisation on Technical

Requirements for Registration of

Pharmaceuticals for Human Use, Q2A,

Text on Validation of Analytical

Procedures, Step 4 of the ICH process,

and Q2B, Validation of Analytical

Procedures: Methodology, Step 4 of the

ICH process, 1996, ICH Steering

Committee.

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Published

31-03-2011

How to Cite

1.
Amene Alvari, Mehrnaz S. Ohadi Rafsanjani, Farhan J. Ahmed, Mohd S. Hejazi, Malik Z. Abdin. Rapid RP-HPLC technique for the determination of phyllanthin as bulk and its quantification in Phyllanthus amarus extract. ijp [Internet]. 2011 Mar. 31 [cited 2024 Dec. 6];3(1):115-9. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/86

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Original Research Articles