Anti-viral and in-vitro free radical scavenging activity of leaves of Rubia cordifolia
Keywords:
Antiviral and Microbial activity, Phytochemical and Pharmacognostical screening, DPPH, free radical scavenging activity, Rubia cordifoliaAbstract
The aim of this research was to develop the pharmacognostical parameters and phytochemical screening along with histological studies and the leaf powder of Rubia cordifolia. The dried leaves of Rubia cordifolia. (Family: Rubiaceae) were subjected to standardization by following pharmacognostical and phytochemical screening methods. Rubia cordifolia was investigated for preliminary phytochemical analysis and characterization by various instrumental techniques. Methanolic extracts of Rubia cordifolia leaves was very good antibacterial activity and also minimum inhibitory concentrating of different virus using HEL cell cultures, HeLa cell cultures and Vero cell cultures but MIC of Herpes simplex - 1 and 2, vaccinia virus, vesicular stomatitis and Herpes simplex- 1 (TK ACVI) were observed very good antiviral activity of Rubia cordifolia leaves DMSO extracts has good minimum cytotoxic concentration activity and also screening for various pharmaceuticals activities. Such as anti oxidant and microbial activities.
References
Yeum KJ, Aldini G, Chung HY, et al. The
activities of antioxidant nutrients in
human plasma depend on the localization
of attacking radical species. The journal
of Nutrition. 2003;133:2688-2691.
Mondon P, Leclercq L, Lintner K
Evaluation of free-radical scavenger
effects of Helianthus annuus extracts
using new ex vivo tripping methods.
Cosmetics, Aerosols and Toiletries in
Australia. 1999;12:87-98.
Pero RW, Roush GC, Markowitz MM, et
al. Oxidative stress, DNA repair, and
cancer susceptibility. Cancer Detection
and Prevention. 1990;14:555-561.
Heo SJ, Park EJ, Lee KW, et al.
Antioxidant activities of enzymatic
extracts from brown seaweeds.
Bioresource Technology. 1990;96:1613-
Antakar DS, Chinwalla T, Bhatt N. Antiinflammatoryactivity of Rubia cordifolia
Linn. In rats. Indian Journal of
Pharmacology, 1983;15:185-188.
Itokawa H, Takeya K, Mori N, et al.
Studies on anti-tumor cyclic hexapeptides
RA obtained from Rubia Radix,
Rubiaceae on derivatives of RA-V and
their in vivo activities. Chemical and
Pharmaceutical Bulletin. 1984;32:3216-
Sing R, Jain A, Panwar S, et al.
Antimicrobial activity of some natural
dyes. Dyes and Pigments. 2005;66:99-
Rao MGM, Rao CV, Pushpangadan P, et
al. Hepatoprotective effects of rubidin, a
major constituent of Rubia cordifolia
Linn, Journal of ethnopharmacology.
;103:484-490.
Tripathi YB, Sing AV. Role of Rubia
cordifolia Linn. in radiation protection.
Indian Journal of Experimental Biology.
;45(7):620-625.
Son JK, Jung SJ, Jung JH, et al.
Anticancer constituents from the roots of
Rubia cordifolia L. Chemical and
Pharmaceutical Bulletin. 2008;56(2):213-
Itokawa H, Mihara K, Takeya K. Chem.
Pharm.Bull. 1983;31:2353-2358.
Chung MJ, Jous J, Cheng TH. J. Nat.
Prod. 1994;57:313-316.
Han BH, Park MK, Prak YH. Archpharm
Res.1990;13:289-291.
Itokawa H, Qiao Y, Mihara K, Takeya K.
Phyto Chemistry. 1991;30:637-640.
Jong Keun Son, Son Ja Jung, JT Hyun
Jang, Zhe Fang, Choong Soon Lee, Chang
Seob Seo, Dong Cheul Moon, Byung Sun
Min, Mi Ryeo Kim, and Mi Hee WW.
Chem. Pham. Bull. 2008;56(2):213-216.
Kamboj VP. Herbal Medicines. Current
Science. 2000;78:35-39.
www.divineremidies.com accessed on
th AUG 2009.
Mohana Rao GM, Rao CV,
Pushpangandan P, Shirwaikar A.
Hepatoprotective effects of Rubiadin, a
major constituent of Rubia cordifolia
Linn, Journal of Ethnopharmacology.
;103:484-490.
Akhtar MS, Ali M, Madhurima, Mir SR,
Onkar S. New anthraquinones from Rubia
cordifolia roots, Indian Journal of
Chemistry. 2006;45(B):1945-1950.
Kuruuzum-UZ A.E, Guvenalp Z,
Demirezer LO, Berger I, Strotch K. 4’
deoxy iridoids glycosides from
Centranthus longiflorus. 2002;61(8):937-
Mishchenko NM, et al. Chemical
composition and pharmacological activity
of anthraquinones from Rubia cordifolia
cell culture. Pharmaceutical Chemistry
Journal. 2007;41(11):38-41.
The Ayurvedic Pharmacopoeia of India,
Government of India Ministry of Health
and family Welfare Dept. Of Indian
system of Medicine & Homeopathy New
Delhi. 1st edition. 2001;1(1).
Kumar GS, Jayaveera KN, Kumar CK
Ashok, Umachigi P Sanjay,Vrushabendra
Swamy BM, Kumar DV Kishore:
Antimicrobial effects of Indian medicinal
plants against acne-inducing bacteria.
Tropi. J. of Ph. Res. June .2007;6(2):717-
Khandelwal KR, Practical
Pharmacognosy; Techniques and
experiments, first Edition, Nirali
Publication, Pune, 1997;1:146-147.
Harborne JB, Phytochemical Method- A
guide to modern techniques of plant
analysis. 1973;45-46.
Finar IL, Organic chemistry: Stereo
Chemistry & the chemistry of Natural
products. Fifth edition, Long man group
Ltd., 1975;5(2):276.
Jitendra Patel, Kumar GS, Shamim
Qureshi Md, Jena PK; Anthelmintic
activity of Ethanolic extract of whole
plant of Eupatorium Odoratum. L.
International Journal of Phytomedicine
;2:127-132.
Kumar GS, Jayaveera KN, Kumar CK
Ashok, Umachigi P Sanjay,Vrushabendra
Swamy BM, Kumar DV Kishore.
Antimicrobial effects of Indian medicinal
plants against acne-inducing bacteria.
Tropi. J. of Ph. Res. June .2007;6(2):717-
World health organization, Quality
Controls Methods for Medicinal Plant
Materials. Geneva AITBS Publishers and
Distributors, Delhi, 2002;8-70:28-30:38-
:64-73.
Evelson P, Travacio M, Repetto M.
Evaluation of total reactive antioxidant
potential of tissue homogenates and their
cytosols. Archives Biochemistry and
Biophysics, 2001;388:261–266.
Cao G, Prior RL. Measurement of oxygen
radical absorbance capacity in biological
samples. Methods in Enzymology,
;299:50–62.
Ou B, Hampsch-Woodill M, Prior RL.
Development and validation of an
improved oxygen radical absorbance
capacity assay using fluorescein as the
fluorescent probe. Journal of Agriculture
and Food Chemistry, 2001;49:4619–4626.
Benzie IFF, Strain JJ. The ferric reducing
ability of plasma as a measure of
‘antioxidant power’’: the FRAP assay.
Analytical Biochemistry, 1996;239:70-76.
Nisar A, Hina F, Bilal HA, Shahid F.
Efficient free radical scavenging activity
of Ginkgo biloba, Stevia rebaudiana and
Parthenium hysterophorous leaves
through DPPH (2, 2-diphenyl-1-
picrylhydrazyl). International journal of
Phytomedicine, 2010;(2):231-239.
Brand-Williams W, Cuvelier ME &
Berset C. Use of a free radical method to
evaluate antioxidant activity. Food
Science and Technology, 1995;28:25–30.
Sa´nchez-Moreno JA, Larrauri & SauraCalixto F. A procedure to measure the
antiradical efficiency of polyphenols.
Journal of the Science of Food and
Agriculture,1998;76:270–276.
Tepe B, & Sokmen, A. Screening of the
antioxidative properties and total
phenolic contents of three endemic
Tanacetum subspecies from Turkish flora.
Bioresource Technology, 2007;98:3076–
Dastmalchi, K., DamienDorman, HJ,
Laakso I & Hiltunen R. Chemical
composition and antioxidative activity of
Moldavian balm (Dracocephalum
moldavica L.) extracts. Food Science and
Technology, 2007;40:1655–1663.
Lim YY & Murtijaya J. Antioxidant
properties of Phyllanthus amarus extracts
as affected by different drying methods.
Food Science and Technology,
;40:1664–1669.
Beauchamp C, Fridovich I.,Anal biochem.
;44:276.
Moncada A, Palmer RMJ, Higgs EA.
Pharmacol Rev, 1991;43:109.
Marcocci L, Packer L, Droy-Lefaiz MT,
Sekaki A, Albert FM. Methods in
Enzymol, 1994;234:462.
Rao Sreejayan MNA. J. of Pharmacol,
;49:105.
Parmar KA, Patel AN, Prajapati SN, Patel
RI; Anti viral in HEL cell, HeLa cell
cultures, antibacterial and antioxidant
activity of Acacia arabica seeds extracts
by the use of DPPH free radical method.
J. Chem. Pharm. Res. 2010;2(4):324-332.
Parmar KA, Prajapati SN, Jani AP.
Phytochemical screening of Randia
dumetoram plant extract for the
antioxidant activity by DPPH method and
their antibacterial and anti-viral activities.
Int. J. Chem. Science 2010;8(2):1101-
