Phytochemical Screening and Antimicrobial Activity of the Leaf Extract of Mirabilis jalapa Against Pathogenic Microorganisms

Authors

  • V. Kiran Kumar Microbiology laboratory, Apex Biosciences, Himayathnagar, Hyderabad – 500029
  • N. Ravi Sankar Microbiology laboratory, Apex Biosciences, Himayathnagar, Hyderabad – 500029
  • S. Ramya Directorate of Sorghum Research, Rajendranagar, Hyderabad – 500030
  • R.V. Sahaja Microbiology laboratory, Apex Biosciences, Himayathnagar, Hyderabad – 500029
  • K. Saritha Department of Biochemistry, University of Hyderabad, Hyderabad – 500046
  • K. Govinda Reddy Microbiology laboratory, Apex Biosciences, Himayathnagar, Hyderabad – 500029
  • N. V. Naidu Global Institute of Biotechnology, Himayathnagar, Hyderabad – 500029

Keywords:

Mirabilis jalapa, leaf extract, phytochemicals, antimicrobial activity

Abstract

Investigation of the phytochemical constituents and antimicrobial activity of the leaf extracts of Mirabilis jalapa were carried out using acetone, chloroform, ethanol and methanol. These extracts were subjected to screening of preliminary phytochemical tests. Phytochemical analysis showed the presence of alkaloids, flavanoids, phenols, glycosides, tannins, saponins and lignins. The methanol extract exhibited the largest zone of inhibition (21mm in dia with 500µg/disc extract) against Staphylococcus aureus and the highest inhibition of fungal radial mycelial growth (97.5% with 500µg/ml medium) against Aspergillus flavus. The methanol extract exhibited the lowest MIC against Staphylococcus aureus (39 µg/ml) and Aspergillus flavus (45µg/ml). It appeared that M. jalapa could be a potential natural source of new antimicrobial agent.

References

Galal M, Bashir AK, Salih AM, Adam SEI.

Activity of water extracts of Albizia

anthelmintica and A. lebbek barks against

experimental Hymenolepis diminuta infection

in rats. J. Ethnopharmacol, 1991; 31: 333-

Ahmed AMA, Rahman MS, Anwar MN.

Antimicrobial activity of extracts and crude

alkaloids isolated from leaf of Adhathoda

vasica Nees. Bangladesh J Life Sci. 2002;

(2): 125 – 128.

Krishnaraju AV, Rao VN, Sundararaju D,

Vanisree M, Subbaraju GV. Assessment of

bioactivity of Indian medicinal plant using

brine shrimp lethality assay. Int. J. Appl. and

Eng. 2005; 3(2): 125-134.

Ravikumar, Patil HS, Makari HK,

Gurumurthy H. In vitro antimicrobial activity

of ethanol extract of Thevetia peruviana.

Electronic J Environ. Agri. Food Chem.,

; 6(9): 2318-2322.

Pratima Mathad, Sundar SM. Phytochemical

and antimicrobial activity of Digera muricata

L Mart. E – Journal of Chemistry. 2010; 7(1):

– 280.

Muthumani P, Devi P, Meera R, Kameshwari,

B, Eswarapriya B. In vitro antimicrobial

activity of various extracts of Mirabilis jalapa

leaves. The Internet Journal of Microbiology,

; 7(2): 120-124.

Ravi Sankar N, Sreeramulu A. In vitro

efficacy of nineteen medicinal plant extracts

on Uncinula tectonae, the powdery mildew of

teak. J Biomed. 2009; 3(2): 137-141.

Harborne JB. Phytochemical methods: A

guide to modern techniques of plant analysis.

rd edition. 1998; Chamman of Hall Pub.

London, UK.

Ergene A, Guler P, Tan S, Mirici S,

Hamzaoglu E, Duran A. Antibacterial activity

and antifungal activity of Heracleum

sphondylium sub sp. artivinense. Afri. J

Biotechnology, 2006; 5(11): 1087-1089.

Miah MAT, Ahmed HU, Sharma NR, Ali A,

Miah SA. Antifungal activity of some plant

extracts. Bangladesh J Bot. 1990; 19(1): 5-10.

Tsuchiya H, Sato M, Miyazaki T, Fujiwara S,

Tanigaki S, Ohyama M, Tanaka T, Iinuma M.

Comparative study on the antibacterial

activity of phytochemical flavanones against

methicillin resistant Staphylococcus aureus.

Journal of Ethnopharmacol. 1996; 50: 27-34.

Ya C, Gaffney SH, Lilley TH, Haslam E.

Carbohydrate-polyphenol complexation.

P.553. In: Hemingway RW, Karchesy JJ (ed),

, Chemistry and Significance of

condensed tannins. Plenum Press, New York.

Nabila B, Mirici S, Duran A. Antioxidant and

antimicrobial activities of the Pistacia

lentiscus and Pistacia atlantica extracts. Afri.

J Pharmacol., 2008; 2(2): 22-28.

Rahman MS, Begum J, Chowdhury JU and

Anwar MN Antimicrobial activity of

Holarrhena antidysenterica against

Salmonella typhi. Chittagong Univ. J. Sci.

; 22(1): 111-112.

Mahesh B, Satish S. Antimicrobial activity of

some important medicinal plant against plant

and human pathogens. World J. Agri. Sci.,

; 4: 839-843.

Gandhiraja N, Sriram, Meena V, Kavitha C,

Sasikumar C, Rajeswari R. Phytochemical

screening and antimicrobial activity of the

plant extracts of Mimosa pudica L. against

the selected microbes. Ethanobotanical

Leaflets. 2009; 13: 618-624.

Anwar MN, Singha PJ, Chowdary JU.

Antifungal activity of some plant extracts on

phytopathogenic fungi. Bangladesh J. Life

Sci. 1994; 6(2): 23-26.

Doughari JH, Obidah JS. In Vitro antifungal

activity of stem bark extracts of Leptadenia

lancifolia. Int. J Integrative Biology, 2008;

(2): 111-117.

Downloads

Published

31-12-2010

How to Cite

1.
V. Kiran Kumar, N. Ravi Sankar, S. Ramya, R.V. Sahaja, K. Saritha, K. Govinda Reddy, N. V. Naidu. Phytochemical Screening and Antimicrobial Activity of the Leaf Extract of Mirabilis jalapa Against Pathogenic Microorganisms. ijp [Internet]. 2010 Dec. 31 [cited 2024 Dec. 6];2(4):402-7. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/64

Issue

Section

Original Research Articles