Anticancer and Antioxidant activity of Tephrosia calophylla against cancer cell lines
Keywords:Anticancer activity, Tephrosia calophylla, IC50 value, MTT assay and antioxidant activity
To evaluate anticancer and antioxidant potentials of activity of methanol extract of Tephrosia calophylla. The methanol extract of Tephrosia calophylla was prepared and tested for in vitro anticancer activity by using human MCF 7, HCT-116, HEP-G2, A-549 cancer and vero normal cell lines. The antioxidant activity for the extract was evaluated by Superoxide scavenging, Lipid peroxidation and DPPH methods. IC50 values of extracts were parameters in both studies. The results of the study have shown that methanol extract of Tephrosia calophylla has shown significant IC50 values against MCF 7, HCT-116 and A-549 cell lines which shows its anticancer potentials but inhibitory effect of methanol extract against HEP-G2 and vero cell lines was not significant. The results also suggest that the methanol extract of Tephrosia calophylla has significant antioxidant properties against Superoxide scavenging, Lipid peroxidation and DPPH methods. The present study shows anticancer potentials of methanol extract of Tephrosia calophylla against MCF 7, HCT-116 and A-549 cell lines.
Uma Devi P, Selvi S, Devipriya D, Murugan S, Suja S, Antitumor and antimicrobial activities and inhibition of in-vitro lipid peroxidation by Dendrobium nobile. African Journal of Biotechnology 2009; 8 (10): 2289-93. 2.Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T, Henkelman RM, Cusimano MD, Dirks PB. Identification of human brain tumour initiating cells. Nature. 2004;18;432(7015):396-401. 3.Bao S, Wu Q, Sathornsumetee S, Hao Y, Li Z, Hjelmeland AB, Shi Q, McLendon RE, Bigner DD, Rich JN. Stem cell–like glioma cells promote tumor angiogenesis through vascular endothelial growth factor. Cancer research. 2006; 66(16): 7843-48. 4.Garcia M, Jemal A, Ward E, Center M, Hao Y, Siegel R, Thun M. Global cancer facts & figures.Atlanta, GA: American Cancer Society. 2007; 1(3). 5.Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F. International Agency for Research on Cancer; Lyon, France: 2013; Cancer incidence and mortality worldwide: GLOBOCAN 2012 v1.0, IARC Cancer Base No. 11 6. Kim EJ, Park SY, Lee JY, Park JH. Fucoidan present in brown algae induces apoptosis of human colon cancer cells. BMC astroenterol. 2010; 10:96. doi: 10.1186/1471-230X-10-96. 7.Siegel R., Naishadham D., Jemal A. Cancer statistics. CA Cancer J.Clin. 2012;62:10–29. 8.Yuan YV, Walsh NA.Antioxidant and antiproliferative activities of extracts from a variety of edible seaweeds. Food and Chemical Toxicology.2006; 44(7): 1144-50. 9.Joy PP, Thomas J, Samuel Mathew, Baby P Skaria, Medicinal plants, Kerala Agricultural University, Kerala, India 1998; 1-9. 10.Baranwal A, Mazumder A, Chakraborthy GS and Gupta S. Phytopharmacological uses of tephrosia purpurea - a review. Pharmacophore 2014;5(4): 658-65. 11.Saad T, Muhammad AS , Muhammad A. A Review on the Phytochemistry and Pharmacology of Genus Tephrosia. Phytopharmacol 2013;4(3): 598-637. 12. GL Viswanatha, Santosh Kumar V, Ramesh C, Nandakumar K and Srinath R. Antioxidant and antimutagenic activities of bark extract of Terminalia arjuna. Asian Pac. J Trop. Med.2010; 8:412-420 13.Kokate CK. Practical Pharmacognosy. Vallabh Prakashan New Delhi: 1994;4:110-1. 14.Khandelwal KR, Practical Pharmacognosy-techniques and experiments. Pune; Nirali Prakashan; 2000. 15. Kai M, Klaus HV, Sebastian L, Ralf H, Andreas R, Ulf-Peter H. Determination of DPPH radical oxidation caused by methanolic extracts of some microalgal species by linear regression analysis spectrophotometric measurements. Sensors 2007; 7: 2080-95. 16.Rajbir S, Saroj A. In vitro evaluation of peroxyl radical scavenging capacity of water extract/fractions of Acacia nilotica (L.) Willd. Ex Del. African J Biotechnol 2009; 8 (7): 1270-2. 17.Rupachandra S and Sarada DVL. Anticancer activity of methanol extract of the seeds of Borreria hispida and Momordica dioica. J pharmacy res. 2013;6: 565-8. 18.Yahaya G et al. Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola). Asian Pac J Trop Med 2014; 7(Suppl 1):355-63. 19.Shyamala V, Ebciba. C, Santhiya. R, Thangaraju N. Phytochemical Screening and In Vitro Antibacterial, Antioxidant and Anticancer Activity of Amphiroa Fragilissima (Linneaus) J V Lamoroux. Int JInn Res Sci Eng Tech. 2014; 3(5) :12933-48. 20.Olinski R, Gackowski D, Foksinski M, Rozalski R, Roszkowski K, Jaruga P. Oxidative DNA damage: assessment of the role in carcinogenesis, atherosclerosis and acquired immunodeficiency syndrome. Free Radical Bio Med 2002; 33(9): 192-200. 21.Smith MA, Perry G, Pryor WA. Causes and consequences of oxidative stress in Alzheimer’s disease. Free Radic Biol Med 2002; 32: 1049-56. Brown JP. A review of the genetic effect of naturally occurring flavonoids, anthraquinones and related compounds. Mutat Res 1980;75:2437. 22.Hirano TK, Oka M, Akiba M. Antiproliferative effect of synthetic and naturally occurring flavonoids on tumour cells of human breast carcinoma cell lines, ZR-75-1. Res Commun Chem Pathol Pharmacol 1989; 64: 69-78. 23.Khan PK, Sinha SP. Antimutagenic profile of antioxidant vitamins in drosophila mutation test. Biomed Environ Sci 2008; 21: 163-6. 24.Senthilkumar R, Manivannan R, Balasubramaniam A, Sivakumar T, Rajkapoor B. Effects of ethanol extract of Pisonia aculeate Linn. on ehrlich ascites carcinoma tumour bearing mice. Int J Green Pharm 2008; 2(1): 50-3. 25.Jostein D, Egil K, Trond S. Bystander effects in UV-induced genomic instability: Antioxidants inhibit delayed mutagenesis induced by ultraviolet A and B radiation. J Carcinogenesis 2005; 4(11): 1-9. 26.Demetrios S, Spyridon O, Demetrios K. Plant phenolics protect from bleomycin-induced oxidative stress and mutagenicity in Salmonella typhimurium TA102. Anticancer Research 2004; 24(2B):743-6.