Chromatographic estimation of maturity based phytochemical profiling of Ipomoea mauritiana


  • M. Sajjad Khan Department of Pharmaceutical Sciences, Dr. H. S. Gour Vishwavidyalaya, Sagar-470 003, Madhya Pradesh, India
  • Nitin Nema Department of Pharmaceutical Sciences, Dr. H. S. Gour Vishwavidyalaya, Sagar-470 003, Madhya Pradesh, India.
  • M.D. Kharya Department of Pharmaceutical Sciences, Dr. H. S. Gour Vishwavidyalaya, Sagar-470 003, Madhya Pradesh, India
  • Salma Khanam Al-Ameen College of Pharmacy, Bangalore-560 028, Karnataka, India.


Microscopy, HPLC, HPTLC, proximate analysis, phytochemical screening


Collection of herbs at right maturity is one of such parameter which affect afficacy of medicinal plants. Standard reference markers used in quality control of herbal drugs mostly authenticate identity and not efficacy. In order to derive bioactive markers, knowledge regarding appropriate collection time for each herb is essential. Traditional medical knowledge is bioactivityoriented and informs about best time of collection for certain medicinal species, as observed in case of Ipomoea mauritiana Jacq. (Vidari–Sanskrit). Only mature (bigger size) tubers of Ipomoea mauritiana are used by Traditional Medical Practitioners (TMP) for preparing galactagogues and immunomodulatory herbal medicines (Rasayan). Microscopy of transverse sections revealed structural variation between mature and immature tubers and girth of tubers determine the maturity of plant, the difference in phytochemical profiles of mature and immature tubers was observed. Variation in phytoconstituents of mature and immature tubers was confirmed through proximate analysis, phytochemical screening, qualitative HPLC and HPTLC analysis revealed the variation in phytoconstituents in mature and immature tubers.

Author Biography

M. Sajjad Khan, Department of Pharmaceutical Sciences, Dr. H. S. Gour Vishwavidyalaya, Sagar-470 003, Madhya Pradesh, India



Schepetkin AI, Quinn MT. Botanical

polysaccharides Macrophage Immunomodulation

and therapeutic potential. Inter. J.

Immunopharmacol. 2006, 6:317-33.

Prats A, Harborne JB. New sources of Ergoline

Alkaloids within the Genus Ipomoea. Biochem.

Sys. Eco. 1993, 214:455-62.

Ferreira AA, Amaral FA, Duarte IDG, Oliveira

PM, Alves RB, Silveira D, Azevedo AO, Raslan

DS, Castro MSA. Antinoceptive effect from

Ipomoea Cairica extract. J. Ethnopharmacol

, 105(1-2): 148-53.

Mungantiwar AA. Studies on the

immunomodulatory effects of Boerhavia diffusa

alkaloidal fraction. J. Ethnopharmacol 1994,


Anonymous. Quality control methods for

medicinal plant materials 1998, WHO, Geneva.

Anonymous. Official methods of analysis

Association of Official Analytical

Chemists, 11th ed. Washington D.C.154.

Dawidar AM, Winternitz F, Johans SR.

Structure of Ipomine, a new alkaloid from

Ipomoea muricata Jacq. Tetrahedron 1999,


Locher CP, Burch MT, Mower HF,

Berestecky I, Davis H, Van B, Lasure A,

Vanden DA, Vlietinck AJ. Anti-microbial

activity and Anti-complement activity of

extracts obtained from selected hawaiian

medicinal plants. J. Ethnopharmacol. 1995,


Dharmaratne HR, Weerawardhena ULBJ,

Herath HMTB, Fujimoto Y. Chemical

investigation of the Methanol extract of root

tubers of Ipomoea mauritiana Chem. Res.

Commun. 1997, 6:39-41.

Zhao G, Kan J, Li Z, Chen Z.

Characterization and Immunostimulatory

activity of an (1-6)-alpa-D-glucan from root

of Ipomoea batatas. Int. J. Immuno. 2005,


Hueza IM, Fonseca ESM, Paulino CA,

Haraguchi M, Gorniak SL. Evaluation of

immunomodulatory activity of Ipomoea

carnea on peritoneal cells of rats J.

Ethanopharmacol. 2003, 87:181-86.

Douglas JA, Smallfield BM, Burgess EJ,

Perry NB, Anderson RE, Malcolm H,

Douglas IAGV. Sesquiterpene lactones in

Arnica montana: A rapid analystical method

and the effects of flower maturity and

stimulated mechanical harvesting on quality

and yield. Planta Med. 2004, 70:166-170.

Narayana K, Iyer MA, Kolammal M:

Pharmacognosy of Ayurvedic Drugs Kerala.

, 1:37-41.

Lamidi M, Rondi ML, Ollivier E, Faure R,

Nzeekekang L, Balansard G. Constituents of

Ipomoea fistulosa leaves Fitoter. 2000,


Davis L, Kuttan G. Immunomodulatory activity of

Withania somnifera. J. Ethnopharmacol. 2000,


Van L, Bodil E, Lindberg F, Lindberg T. Effect of

Harvesting interval and defoliation on yield and

chemical compostion of leaves, stems and tubers

of Ipomoea batatas plant parts. Agric. Sci. 2003,


Bieber LW, Filho AADS, Lima RMOC,

Chiappeta ADA, Nascimento SCD, Souza IAD,

Mello JFD, Veith HJ. Anticancer and

antimicrobial glycosides from Ipomoea bahiensis.

Phytochem. 1996, 25:1077-81.

Souza MD. Antinociceptive properties of the

methanolic extract obtained from Ipomoea Pescaprae (L) R. J. Ethanopharmacol. 2000, 69:85-

Schoeninger MJ, Bunn HT, Marray SS, Mariett

JA. Compostion of tubers used by Hadza foragers

of Tanzania. J. Food Comp. Anal. 2001, 14:15-20.

Saho N. Acylated Petargonidin Glycosides in

Red-purple flowers of Ipomoea purpurea.

Phytochem. 1996, 436:1365-70.

Mann P, Tofern B, Tofern MK, Eich E. Flavonoid

Sulfates from the Convolvulaceae. Phytochem.

, 50:267-71.

Chopra RN, Nayer SL, Chopra IC. Glossary of

Indian medicinal plants, First edition, 3rd reprint,

NISCAIR. 1992, 142.

Srivastava R, Sachdeva K, Madhusudanan KP,

Dinesh K. Kulshveshtha structure of Pescaproside

E. a fatty acid glycoside from Ipomoea

Pescaprae. Carbo. Res. 1991, 212:169-76.

Cambie RC, Fergusion LR: Potential functional

Foods in the traditional Maori Diet. Mut. Res.

, 109-17.

Miranda RP, Cartos BH. HPLC isolation

and structural elucidation of diasteromeric

niloyl ester tetrasaccharides from Mexican

Scammony root Tetrahedron 2002, 58:3145-

Gonda R, Tomoda M, Shimiza N. Strucrure

and Anticomplementary activity of an acidic

polysaccharide from the leaves of Malva

sylvestris var. mauritiana. Carbo. Res. 1990,


Mishra SS, Tiwari JP, Matin A.

Investigation of fixed oil from Ipomoea

digitata tubers. J. Pharma. Sci. 1965,


Schimming T, Jenett-Siems K, Mann P,

Tofern-Reblin B, Milson J, Johnson RW,

Deroin T, Austin DF, Eich E. Calystegines

as chemotaxonomic markers in the

Convolvulaceae. Phytochem. 2005, 66:469-

Chen TE, Huang DJ, Lin YH. Isolation and

characterization of Serine protease from the

storage roots of Sweet potato. Plant Science.

, 166:1019-26.

Tiwari U, Rastogi B, Singh P, Saraf DK, Vyas

SP. Immunomodulatory effects of aqueous

extract of Tridax procumbens in experimental

animals. J. ethnopharmacol. 2004, 92:113-9.

Singh V, Pandey M, Srivastava A, Sethi R. A

non-ionic water soluble seed from Ipomoea

campanculata. Fitoter. 2003, 74:40-41.

Woradulayapinij W, Soonthornchareonnon N,

Wiwat C: In Vitro HIV type 1 reverse

transcriptase inhibitory activities of Thai

medicinal plants and Canna indica L. rhizomes. J.

ethnopharmacol. 2005, 101:84-9.

Lothar WB, Filho AADS, Lima RMOC,

Chiappeta ADA, Nascimento SCD, Souza

IAD, Mello JFD, Veith HJ. Anticancer and

Antimicrobial Glycosides from Ipomoea

bahiensis. Phytochem. 1996, 25:1077-81.

Miyazaki Y, Kusano S, Doi H, Aki O. Effects on

immune response of Anti diabetic ingredients

from white skinned Sweet potato. Nutri. 2005,





How to Cite

M. Sajjad Khan, Nitin Nema, M.D. Kharya, Salma Khanam. Chromatographic estimation of maturity based phytochemical profiling of Ipomoea mauritiana. ijp [Internet]. 2009 Dec. 31 [cited 2024 Feb. 22];1(1):22-30. Available from:



Original Research Articles