Phytochemical composition and in vitro antioxidant activity of methanolic and aqueous extracts of aerial part of Pentatropis nivalis(Asclepiadaceae)

Authors

  • Nilesh P. Babre ViVa Institute of Pharmacy, virar (E), Affilated to University of Mumbai. India
  • T. Shivraj Gouda Department of Pharmacology, NET Pharmacy College, Raichur - 584103, Karnataka, India
  • Narayanswamy Lachmanan Gowrishankar Department of Pharmacognosy, Prime College of Pharmacy, Erattayal, Kodumbu,Palakkad - 678551, Kerala, India

Keywords:

Pentatropis nivalis, Thin Layer Chromatography, Antioxidant activity, DPPH, FRAP

Abstract

The objective of present study was to evaluate preliminary phytoconstitutents and invitro antioxidant potential of Pentatropis nivalis (Asclepiadaceae). During the preliminary phytochemical analysis, methanolic and aqueous extracts of aerial part of Pentatropis nivalis was screened for the presence of phenolic, saponins, flavonoids, alkaloids, tannins and phytosterols. TLC of extracts were performed by using various solvent systems. Phytochemicals screening and TLC spots of MEPN and AEPN showed the presence of glycoside, steroids, terpenoids, phenolic, saponins, Methanolic extract showed better qualitative tests for presence of secondary metabolites than aqueous extract.The in-vitro antioxidant potential of extracts were evaluated by DPPH and FRAP, and both methods showed that the plant possesses good antioxidant activity.

References

Waris G, Ahsan H. Reactive oxygen species: role in the development of cancer and various chronic conditions. J Carcinog 2006; 5(1): 14-21

Zhou C, Huang Y, Przedborski S. Oxidative stress in Parkinson’s disease: a mechanism of pathogenic and therapeutic significance. Ann N Y AcadSci 2008; 1147: 93-104.

Praticò D. Evidence of oxidative stress in Alzheimer’s disease brain and antioxidant therapy: lights and shadows. Ann N Y AcadSci 2008; 1147: 70-78.

Elahi MM, Kong YX, Matata BM. Oxidative stress as a mediator of cardiovascular disease. Oxid Med Cell Longev 2009; 2(5): 259- 269

Wright Jr E, Scism-Bacon JL, Glass LC. Oxidative stress in type 2 diabetes: the role of fasting and postprandial glycaemia. Int J ClinPract 2006; 60(3): 308-314.

Uttara B, Singh AV, Zamboni P, Mahajan RT. Oxidative stress and neurodegenerative diseases: A review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 2009; 7(1): 65-74

Narasimhan S, Shobana R, Sathya TN. Antioxidants- natural rejuvenators that heal, detoxify and provide nourishment.In: Sharma RK, Arora R, editors. Herbal Drugs - A Twenty First Century Perspective. New Delhi: Jaypee Brothers Medical Publishers; 2006; 548-558.

Kirtikar, K. R. and Basu, B. D. (1987). “Indian medicinal plants, 2ndEds, Dehradun, India”, International book distributors and book sellers, pp1614-15.

Harbone JB .Phytochemical Methods. 1st Ed. Chapman and Hall, London. 1973; 60-66.

Kokate CK. Practical pharmacognosy. 4th Ed. VallabhPrakashan, Delhi 1994; 107-111.

Khandelwal K.R.Practical Pharmacognosy Technique& Experiments, NiraliPrakashan, Pune 20th edition; 2010: 23.1-25.9, 17.1

Trease G, Evans SM. Pharmacognosy. 15th ed. London: BailerTindal; 2002, p. 23-67.

Chen Y, Wang MF, Rosen RT, Ho CT. 2, 2-Diphenyl -1 -picrylhydrazyl radicalscavenging active components from PolygonummultiflorumThunb. J. Agric. Food Chem. 1999; 47:2226–2228.

Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of Antioxidant power: The FRAP assay. Anal. Biochem. 1996; 239:70–76.

Bobbit JM. 2nd ed. New York: Verlogchemise Academy Press; 1966. Thin Layer Chromatography.

Randerath K. 2nd ed. New York: Verlogchemise Academy Press; 1966. Thin Layer Chromatography.

Badarinath, A.V., Rao, K.M., Chetty, C.M.S., Ramkanth, V., Rajan, T.V.S., Gnanaprakash, K., 2010. A review on in-vitro antioxidant methods: comparisons, correlations and considerations. Int. J. PharmTech Res. 2 (2), 1276–1285.

Downloads

Published

31-03-2018

How to Cite

1.
Nilesh P. Babre, T. Shivraj Gouda, Narayanswamy Lachmanan Gowrishankar. Phytochemical composition and in vitro antioxidant activity of methanolic and aqueous extracts of aerial part of Pentatropis nivalis(Asclepiadaceae). ijp [Internet]. 2018 Mar. 31 [cited 2024 Oct. 22];10(1):68-72. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/591

Issue

Section

Original Research Articles