Immunomodulatory effects of herbal plants plus melatonin on human blood phagocytes

Authors

  • Eduardo Luzia França Institute of Biological and Health Science - Federal University of Mato Grosso – Pontal do Araguaia – MT, Brazil. - Rodovia MT 100, Km 3,5 s/no, Pontal do Araguaia - MT. CEP:78698-000
  • Joanna Conde Maynié Environmental Biodiversity Center EcoCerrado Reserve, Araxá, MGBrazil.
  • Valéria Conde Correa Environmental Biodiversity Center EcoCerrado Reserve, Araxá, MGBrazil.
  • Uender Correia Rodrigues Pereira Institute of Biological and Health Science - Federal University of Mato Grosso – Pontal do Araguaia – MT, Brazil. - Rodovia MT 100, Km 3,5 s/no, Pontal do Araguaia - MT. CEP:78698-000
  • Claudemir Batalini Federal University of Mato Grosso Pontal do Araguaia – MT, Brazil
  • Carlos Kusano Bucalen Ferrari Institute of Biological and Health Science - Federal University of Mato Grosso – Pontal do Araguaia – MT, Brazil. - Rodovia MT 100, Km 3,5 s/no, Pontal do Araguaia - MT. CEP:78698-000
  • Adenilda Cristina Honorio-França Institute of Biological and Health Science - Federal University of Mato Grosso – Pontal do Araguaia – MT, Brazil. - Rodovia MT 100, Km 3,5 s/no, Pontal do Araguaia - MT. CEP:78698-000

Keywords:

herbal mixture, melatonin, blood phagocyte, phagocytosis, superoxide

Abstract

It has been shown a mixture of seven herbal plants was able to trigger cell oxidative mechanism and subsequently inducing cellular activation. Moreover, melatonin hormone has also been shown to perform different actions on the cellular oxidative metabolism. It is possible that the herbal mixture associated with melatonin can activate phagocytes, improving microbicidal activity and further ameliorating resistence to the infections. The aim of this work was to verify in vitro immunomodulatory effects of melatonin and a medicinal plants mixture on blood mononuclear phagocytes (MN). We collected 40 blood samples from normal individuals to obtain the phagocytes. The MN phagocytes were separated by Ficoll-Paque gradient. Preparation of plant extract to obtain the herbal mixture was carried through the process of maceration followed by distillation. Phagocytosis and microbicidal activity of blood phagocytes, treated or not with exogenous superoxide dismutase (SOD), against enterophathogenic Escherichia coli (EPEC) were evaluated by acridine orange method. The herbal mixture and/or melatonin were added to the cell suspensions as immunomodulators. We observed increased phagocytosis and microbicidal activities by blood MN phagocytes in the presence of melatonin or the herbal mixture. The association of both potentiated the functional activity of blood MN phagocytes. Phagocytes previously treated by exogenous SOD had decreased microbicidal activity independently of immunomodulators. These data suggest that the herbal mixture is a potent immunostimulatory agent, and that the interaction between plant and hormones may represent an alternative mechanism of defense against infection, especially in immunosuppressed patients.

References

Asad NR, Assad LMBO, Almeida CEB,

Leitão, A.C. Lethal interaction between

hydrogenperoxide and 0-phenonthroline in

Escherichia coli. Br J Med Biol Res 1994; 27:

-2555.

Honorio-França AC, Carvalho MPSM, Isaac

L, Trabulsi LR, Carneiro-Sampaio MMS.

Colostral mononuclear phagocytes are able to

kill Enteropathogenic Escherichia coli

opsonized with colostral IgA. Scand J

Immunol 1997;46: 59-66.

Honorio-França AC, Launay P, CarneiroSampaio MMS, Monteiro RC. Colostral

neutrophils express IgA Fc receptors (CD89)

lacking y chain association that mediate noninflammatory properties of secretory IgA. J

Leuk Biol 2001; 69: 280-288.

França EL, Pereira Jr A, Oliveira SL,

Honorio-França AC.

Chronoimmunomodulation of melatonin on

bactericidal activity of human blood

phagocytes. Intern J Microbiol 2009; 6:1-15.

Ames BN, Shinnenaga MK, Hagen TM.

Oxidants, antioxidantes, and the degenerative

diseases of agent. Proc Nat Acad Sci 1993;

: 7915-7922.

Tiwari U, Rastogi B, Singh P, Saraf DK, Vyas

SP. Immunomodulatory effects of aqueous

extract of Tridax procumbens in experimental

animals. J Ethnopharmacol 2004; 92: 113-

Atal CK, Sharma ML, Kaul A, Khajuria A.

Immunomodulating agents of plant origin. I:

Preliminary screening. Journal

Ethnopharmacol. 1986; 18:133-134.

Viegas Jr C, Bolzani VS, Barreiro EJ. The

natural products and the modern medicinal

chemistry. Quím Nova 2006; 29:326-337.

Corrêa VSC, Maynié JC, França EL, HonorioFrança AC. Activity of phagocytes in the

Presence of the “Mais Vida” (more life)

Herbal Remedy. Braz J Med Plants 2006; 8:

-32.

França EL, Feliciano ND, Silva KA, Ferrari

CKB, Honorio-França AC. Modulatory Role

of Melatonin on Superoxide Release by

Spleen Macrophages Isolated from AlloxanInduced Diabetic Rats.. Bratisl Med J

;7:163-173.

Dardenne M, Savino W. Control of thymus

physiology by peptidic hormones and

neuropeptides. Immunol Today 1994; 15: 519-

Cassone VM. The pineal gland influences rat

circadian activity rhythms in constant light. J.

Biol. Rhythms 1992; 7: 27-40.

Claustrat B, Brun J, Chazot G. The basic

physiology and pathophysiology of melatonin.

Sleep Med Rev 2005; 9:11–24.

Pandi-Perumal Sr, Trakht I, Srinivasan V,

Spence DW , Maestroni GJM , Zisapel N.

Physiological effects of melatonin: Role of

melatonin receptors and signal transduction

pathways. Prog Neurobiol 2008; 85: 335–353.

Honorio-França AC, Silva KA, Feliciano ND,

Calderon IMP, Rudge MVC, França EL.

Melatonin effects on macrophage in diabetic

rats and the maternal hyperglycemic

implications for newborn rats. Int J Diabet

Metabol 2009; 17:87-92.

Ianãs O, Olinescu R, Bãdescu I. Melatonin

involvement in oxidative processes. Rom J

Endocrinol 1991; 29:147-153.

Finocchiaro LME, Nahmod VE, Launay JM.

Melatonin biosíntesis and metabolism in

peripheral blood mononuclear leucocytes.

Biochem J 1991; 280:727-731.

Harbone, J.B. 1984. Phytochemical Methods.

A guide to Modern Techniques of Plant

Analysis, Second ed. Chapman and Hall,

London, pp. 84-274.

Pawlak J, Singh J, Lea RW, Skwarlo-Sonta K.

Effect of melatonin on phagocytic activity and

intracellular free calcium concentration in

testicular macrophages from normal and

streptozotocin-induced diabetic rats. Mol Cell

Biochem 2005; 275: 207-213.

Bellinati-Pires R, Salgado MM, Hypolito IP,

Grumach AS, Carneiro-Sampaio, MMS.

Aplication of a fluorochrome-lysostaphin

assay to the detection of phagocytic and

bactericidal disturbances in human neutrophils

and monocytes. J Investig Allergol Clin

Immunol. 1995; 5:337-342.

Babu U, Failla ML. Copper status and fuction

of neutrophlis are reversibly depressed in

marginally and severely copper-deficient rats.

J Nutr 1990; 120:1700-1709.

Rêgo, TJA. Fitogeografia das plantas

medicinais no Maranhão. 2nd ed. EDUFMA,

MA, 108-109, 1995.

Calixto, J.B. Efficacy, safety, quality

marketing and regulatory guidelines for herbal

medicines (phytotherapeutic agents). Braz J

Med Biol Res 2000; 32: 179-189.

Hasani-Ranjbar S, Larijani B, Abdollah MA.

Systematic review of Iranian medicinal plants

useful in diabetes mellitus. Arch Med Sci

;.4: 285-292.

Bussmann RW, Meyer AGK, Kuhlman A,

Townesmith A. Herbal mixtures in traditional

medicine in Northern Peru. J Ethnobiol

Ethnomedicine 2010; 6:2-11.

Honorio-França AC; Marins C. Boldrini F,

França EL. Evaluation of hypoglicemic

activity and healing of extract from amongst

bark of "Quina do Cerrado" (Strychnos

pseudoquina ST. HILL). Acta Cir Braz 2008;

: 504-510.

Naser B, Lund B, Henneicke-Von Zepellin

HH, Köhler G, Lehmacher W, Scaglione F. A

randomized, double-blind, placebo-controlled,

clinical dose-response trial of an extract of

Baptisia, Echinacea and Thuja for the

treatment of patients with common cold.

Phytomedicine. 2005;12:715-722.

Besedovsky HO, Del Rey A. Immune-neuroendocrine interactions: Facts and hypotheses.

Endocr Rev 1996; 17: 64–102.

Klepac N, Rudes Z,Klepac R. Effects of

melatonin on plasma oxidative stress in rats

with streptozotocin induced diabetes. Biomed

Pharmacother 2005; 60: 32-35.

Sudnikovich EJ, Maksimchik YZ,

Zabrodskaya SV, Kubyshin VL, Lapshina EA,

Bryszewska M. Melatonin attenuates

metabolic disorders due to streptoxotocininduced diabetes rats. Eur J Pharmacol 2007;

: 180-187.

Manosroi A, Saraphanchotiwitthaya A,

Manosroi J. Immunomodulatory activities of

Clausena excavata Burm. f. wood extracts. J

Ethnopharmacol 2003; 89: 155-160.

Gokhale AB, Damre AS, Saraf MN.

Investigations into the immunomodulatory

activity of Argyreia speciosa. J

Ethnopharmacol 2003; 84: 109-114.

França-Botelho AC, Honorio-França AC,

França EL, Gomes MA, Costa-Cruz JM.

Phagocytosis of Giardia lamblia trophozoites

by human colostral leucocytes. Acta Paediat

; 95: 438-443.

Kang NS, Park SY, Lee SM, Lee BG, Shin

DH, Pyo S. 2002. Modulation of macrophage

function activity by ethanolic extract of larvae

of Holotrichia diomphalia. J Ethnopharmacol

; 79: 89-94.

Traber MG. Cellular and molecular

mechanisms of oxidants and antioxidants. Min

Electrol Metabol 1997; 23: 135-139.

Gort AS, Imlay JA. Balance between

endogenous superoxide stress and antioxidant

defense. J Bacteriol 1998; 180:1402-1410.

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Published

31-12-2010

How to Cite

1.
Eduardo Luzia França, Joanna Conde Maynié, Valéria Conde Correa, Uender Correia Rodrigues Pereira, Claudemir Batalini, Carlos Kusano Bucalen Ferrari, Adenilda Cristina Honorio-França. Immunomodulatory effects of herbal plants plus melatonin on human blood phagocytes. ijp [Internet]. 2010 Dec. 31 [cited 2024 Dec. 6];2(4):354-62. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/58

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