In vitro antioxidant activities of stem bark, leaves and callus extracts from Amoora rohituka

Authors

  • Kanthesh M Basalingappa Division of Molecular Biology Faculty of Life Sciences Jagadguru Sri Shivarathreeshwara University, Mysuru-570015 India
  • Kavitha Raj V Division of Biochemistry, Faculty of Life Sciences, Jagadguru Sri Shivarathreeswara (JSS) University, Mysuru-570015.
  • Avinash KO Division of Biochemistry, Faculty of Life Sciences, Jagadguru Sri Shivarathreeswara (JSS) University, Mysuru-570015.
  • Kumar JR Division of Biochemistry, Faculty of Life Sciences, Jagadguru Sri Shivarathreeswara (JSS) University, Mysuru-570015.
  • Murugesan Karthikeyan Senior Lecturer, Department of Microbiology, Faculty of Medicine, Quest International University Perak, Malaysia.
  • Ashok Gnanasekaran Senior Lecturer, Department of Microbiology, Faculty of Medicine, Quest International University Perak, Malaysia.
  • Chandrashekrappa GK Chairman, Faculty of Life Sciences, Jagadguru Sri Shivarathreeswara (JSS) University, Mysuru-570015.

Keywords:

Amoora rohituka, Antioxidant activity, Petroleum ether,, Chloroform and Methanol

Abstract

The present work examined the in vitro antioxidant activity of sequentially extracted, solvent extracts (Petroleum ether, chloroform and Methanol) of stem bark, leaves and callus from Amoora rohituka. The antioxidative capacity of the extracts were examined by using DPPH (1,1-diphenyl,2-picryl hydrazyl), ABTS (2,2’-azinobis-3-ethyl-benzothiozoline-6-sulphonic acid) and NOS (Nitric oxide scavenging) assays by using Gallic acid as standard control. Among the tested extracts, methanol extract of stem bark showed better antioxidative capacity. The total antioxidant activity was expressed as µg equivalent of Gallic acid per gram on dry weight basis. IC50 values for methanol extract of stem bark were 7.53 µg/ml; 34.92 µg/ml and 56.31 µg/ml in ABTS, DPPH and NOS assays respectively. Whereas, leaves and callus extracts showed moderate antioxidative properties.

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Published

31-12-2017

How to Cite

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Kanthesh M Basalingappa, Kavitha Raj V, Avinash KO, Kumar JR, Murugesan Karthikeyan, Ashok Gnanasekaran, Chandrashekrappa GK. In vitro antioxidant activities of stem bark, leaves and callus extracts from Amoora rohituka. ijp [Internet]. 2017 Dec. 31 [cited 2024 Oct. 15];9(4):584-8. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/571

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