Evaluation of chemical profile and antioxidant potential of Trichodesma indicum (L.) R.Br.


  • Mahendra S Khyade ORCID iD Department of Botany, S.N. Arts, D.J.M. Commerce and B.N.S. Science College, Sangamner- 422605.Affiliated to Savitribai Phule Pune University, Pune (MS), India.
  • Subhash N Varpe Department of Botany, Nutan Arts, Commerce and Science College, Rajapur-422605, India.
  • Anup D Padwal Department of Botany, S.N. Arts, D.J.M. Commerce and B.N.S. Science College, Sangamner- 422605.Affiliated to Savitribai Phule Pune University, Pune (MS), India.


Antioxidant, hydro- methanolic extract, Trichodesma indicum, GC-MS, HPLC


The present study was conducted to evaluate the antioxidant activity and chemical composition of aqueous methanolic extract of Trichodesma indicum (L.) R.Br. The extract was tested for antioxidant activity using various in vitro models viz., DPPH, ABTS, NO, FRAP, total antioxidant activity and reducing power. The total phenolic and flavonoid contents were found to be equivalents to 97.83 ± 0.08 µg of gallic acid and 45.45 ± 0.19 µg of rutin /mg of dried aqueous methanolic extract, respectively. Among various antioxidant assays performed, maximum inhibition was observed in the ABTS (IC50 17.13 ± 0.23 μg) followed by DPPH (IC50 80.64 ± 0.29 μg) and NO (IC50 4.12 ± 0.02 μg) assay. GC-MS analysis revealed the presence of over 38 compounds, the prevailing compounds were hexadecanoic acid, β-sitosterol, ethyl iso-allocholate and 9,12,15,-Octadecatrienoicacid. The HPLC analysis further confirmed the presence of rutin, salicylic acid and benzoic acid. This confirms that T. indicum could be the good source of natural antioxidant for industrial and pharmaceutical preparations.


. Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts, Food Chem, 2004, 85: 231-237.

. Kasote DM, Katyare SS, Hegde MV, Bae, HH. Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int J Biol Sci, 2015, 11: 982-991.

. Anonymous. The Wealth of India, Publications and information Directorate, Council of Scientific and Industrial Research, New Delhi, 1985.

. Kirtikar KR, Basu BD. Indian Medicinal Plants. Srisatguru Publications, New Delhi, 2000.

. Varier SPV. Indian Medicinal Plants, Orient Longman Ltd. Madras, 1993.

. Chopra RN, Chopra IC, Handa KL, Kapur LD. Indigenous Drugs of India, U.N. Dhur and Sons Pvt. Ltd., Calcutta, 1958.

. Agarwal VS. Drug Plants of India, Kalyani Publishers, New Delhi, 1997.

. Hasan M, Ahamad S, Mohamood K. Chemical investigation of Trichodesma indicum leaves. I. Nonsteroidol constituents of the petroleum ether extract. J Chem Soci Pak, 4:281–283.

. Badami RC, Deshpande GS, Shanbhag MR. Minor seed oils. VII. Examination of seed oils by gas–liquid chromatography, J Oil Technol Assoc India, 1975, 7:76–77.

. Srikanth K, Murugesan T, Kumar CA, Suba V, Das AK, Sinha S, Arunchalam G, Manikandan L. Effect of Trichodesma indicum extract on cough reflex induced by sulphur dioxide in mice. Phytomedicine, 2002, 9:75–77.

. Perianayagam JB, Sharma SK, Pillai KK. Evaluation of analgesic and antipyretic potential of Trichodesma indicum root extract in animal models, Int J Pharmaceut Sci Lett, 2012, 1:9-14.

. Reddy SD, Rao SA, Hulikere PA. In Vitro antioxidant and glucose uptake effect of Trichodesma Indicum In L-6 Cell Lines. Int J Pharm Bio Sci, 2012, 3:810 – 819.

. Saboo ISS, Tapadiya GG, Khadabadi ISS. Antimicrobial potential of tropical plant Trichodesma indicum and Trichodesma sedgwickianum. Res J Microbiol, 2013, 8:63-69.

. Mangai SA, Subban R. In-vitro Cytotoxicity of Trichodesma indium L.R.Br., extracts against three human cancer cell lines. Asian J Pharmaceut Clin Res, 2014, 7:103-105.

. Manani LM, Kakrani P, Saluja AK. Evaluation of diuretic activity of Trichodesma indicum R.Br. In Rats. Int J Pharma Bio Sci, 2014, 5:129 – 133.

. Anusha K, Balakrishnan S, Sindhu S, Arumugam P, Sekar BH. Studies on Phytochemical screening and antioxidant potential of Trichodesma indicum. Int J Pharmacog Phytochem Res, 2014, 6:536-539.

. Sangeetha S, Das MSC, Gayathri Devi S. Comparative evaluation of free radical scavenging activity of Cleome viscosa and Trichodesma indicum. Int J Pharm Pharmaceut Sci, 2014, 6:318-325.

. Cooke T. Flora of Presidency of Bombay, Published under the Authority secretary of State for Council,Culcutta, 1903.

. Pradhan SG, Singh NP. Flora of Ahmednagar District (MS) BSI, Dehradun, India, 1999.

. Harborne JB. Phytochemical methods. Chapman Hall, London, 1984.

. Mahida Y, Mohan JSS. Screening of Indian plant extracts for antibacterial activity, Pharmaceut Biol, 2006, 44:627-631.

. Trease E, Evans WC. Pharmacognosy. Billiare Tindall, London 1987.

. Zou Y, Lu Y, Wei D. Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro, J Agric Food Chem, 2004, 52:5032-5039.

. Bhalerao BM, Kasote DM, Nagarkar BE, Jagtap SD, Vishwakarma SK, Pawar PK, Maheshwari VL. Comparative analysis of radical scavenging and immunomodulatory activities of Tinospora cordifolia growing with different supporting trees. Acta Biol Szeged,(2012, 56:65-71.

. Cliffe S, Fawer M, Maier G. Takata K, Ritter G. Enzyme assays for the phenolic content of natural juices, J Agri Food Chem, 1994, 42:1824-8.

. Khoudja NK, Lila Boulekbache-Makhlouf Khodir Madani. Antioxidant capacity of crude extracts and their solvent fractions of selected Algerian Lamiaceae. Ind Crops Prod, 2014, 52:177–182.

. Prabhakar KR, Veeresh VP, Vipan K, Sudheer M, Priyadarsini KI, Satish RBSS. Bioactivity-guided fractionation of Coronopus didymus: a free radical scavenging perspective, Phytomedicine, 2006, 13: 591–595.

. Yen GC, Chen HY. Antioxidant activity of various tea extracts in relation to their anti-mutagenicity, J Agri Food Chem, 1995, 43:27-32.

. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med, 1999, 26:1231-7.

. Ilavarasan R, Mallika M, Venkataraman S. Anti-inflammatory and antioxidant activites of Cassia fistula Linn. bark extract. African Journal of Traditional, Complementary and Alternative Medicine, 2005, 1:70-85.

. Benzie IF, Strain JJ. Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol, 1999, 299:15-27.

. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem, 1999, 269:337-41.

. Shirwaikar A, Rajendran K, Kumar CD. In vitro antioxidant studies of Annona squamosa Linn. leaves, Indian J Exp Biol, 2004, 42:803-807.

. Janakiraman N, Sathish SS, Johnson M. V­VIS and FT-IR spectroscopic studies on Peristroph bicalyculata (Retz.) Nees. Asian J Pharm Clin Res, 2011, 4:125­-129.

. Olajide OA, Aderogba MA, Adedapo AD, Makinde JM. Effects of Anacardium occidentale stem bark extract on in vivo inflammatory models, J Ethnopharmacol 2004, 95(2-3):139-42.

. Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea, Carcinogenesis, 1989, 10(6):1003-8.

. Ogunleye DS, Ibitoye SF. Studies of antimicrobial activity and chemical constituents of Ximenia americana, Trop J Pharm Res, 2003, 2(2):239-41.

. Cheeke PP. Surprising benefits of desert plants. Corvallis, Oregon: Oregon University, in the Linus Paulin Institute,2005.

. Rice-Evan, CA, Miller NJ, Bollwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic favonoids, Free Radical Res, 1995, 22:375-383.

. Pietta DG. Flavonoids as antioxidants. J Nat Prod, 2000, 63:1035-1042.

. Simirgiotis MJ. Antioxidant capacity and HPLC–DAD-MS profiling of Chilean Peumo (Cryptocarya alba) fruits and comparison with German Peumo (Crataegus monogyna) from Southern Chile. Molecules, 2013, 18: 2061–2080.

. Gouveia S, Gonc alves J, Castilho PC. Characterization of phenolic compounds and antioxidant activity of ethanolic extracts from flowers of Andryala glandulosa ssp. varia (Lowe ex DC.) R.Fern., an endemic species of Macaronesia region, Ind Crops Prod, 2013, 42:573–582.

. Xi W, Zhang Y, Sun Y, Shen Y, Ye X, Zhou Z. Phenolic composition of Chinese wild mandarin (Citrus reticulata Balnco.) pulps and their antioxidant properties, Ind Crops Prod, 2014, 52:466–474.

. Chidambaram A, Ajithadas A. Pharmacological evaluation of methanolic extract of Trichodesma indicum (Linn) R.Br. Asian J Pharm Clin Res, 2013, 6:72-75.

. Ialenti A, Moncada S, Di Rosa M. Modulation of adjuvant arthritis by endogenous nitric oxide. Br J Pharmacol, 1993, 110:701‑706.

. Contreras-Calderón, J., Calderón-Jaimes L., Guerra-Hernández, E., García-Villanova, B., (2011). Antioxidant capacity, phenolic content and vitamin C in pulp, peel and seed from 24 exotic fruits from Colombia. Food Res Int, 2011, 44: 2047–2053.

. Bhatti M, Kamboj A, Saluja A, Jain UK. In vitro evaluation and comparison of antioxidant activities of various extracts of leaves and stems of Kalanchoe pinnatum. Int J Green Pharm, 2012, 6: 340-347.

. Lin XH, Wu YB, Lin S, Zeng JW, Zeng PY, Wu JZ. Effects of volatile components and ethanolic extract from Eclipta prostrata on proliferation and differentiation of primary osteoblasts, Molecules, 2010, 15:241–250.

. Ishihara Y, Katayama K, Sakabe M, Kitamura M, Aizawa M, Takara M, Itoh K. Antioxidant properties of rare sugar D-allose: Effects on mitochondrial reactive oxygen species production in Neuro2A cells, J Biosci Bioeng, 2011, 112:638–642.

. Yang J, Qu Z, Xiao YL, Qiu GF, Zhang T, Wu ZY, He XR, Hu XM. Chemical composition and antioxidant activity of the essential oil of endemic Viola tianshanica. Nat Prod Res, 2011, 25:1635–1640.

. Zhang W, Shen H, Ma L, Shen B, Xu Z, Wang X. Differential expression of peroxiredoxin 6 in fetal rat testis following in utero exposure to di(n-butyl) phthalate, Toxicology, 2007, 240:86–95.

. Bowyer JN, Rout-Pitt N, Bain PA, Stone DA, Schuller KA. Dietary fish oil replacement with canola oil up-regulates glutathione peroxidase 1 gene expression in yellowtail kingfish (Seriola lalandi ). Comp Biochem Physiol B Biochem Mol Biol, 2012, 162:100–106.




How to Cite

Mahendra S Khyade, Subhash N Varpe, Anup D Padwal. Evaluation of chemical profile and antioxidant potential of Trichodesma indicum (L.) R.Br. ijp [Internet]. 2017 Sep. 30 [cited 2024 Jun. 17];9(3):416-25. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/553



Original Research Articles