Anti-leukemic activity of phosphoproteins from Sesamin via induction of nuclear antigen H731and CLIP-associating protein 2 isoform X25 mediated apoptosis


  • Pattharin Wannapruk Department of Clinical microscopy, Faculty of Medical Technology, Mahidol University
  • Atchara Paemanee National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)
  • Sittiruk Roytrakul National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)
  • Dalina I Tanyong Department of Clinical microscopy, Faculty of Medical Technology, Mahidol University


Leukemia, Sesamin, Apoptosis, Phosphoprotein, Mass spectrometry


Leukemia is an uncontrolled proliferation hematopoietic cancer cells that commonly treats with conventional therapies such as chemotherapy. However, many side effects were reported. Alternative medicines have been developed by using natural or herb compounds as therapeutic drug. Sesamin, a class of phytoestrogen isolated from sesame seed displaying potent anticancer activity in vitro and in vivo.However, the mechanism by which sesamin mediates anticancer effects on leukemic cells are notfully understood. In thisstudy, the effects of sesamin on cell viability, cell apoptosis and expression of phosphoproteins in Molt-4 and NB4 leukemic cell lines were investigated using MTT assay, flow cytometry and immobilized metal affinity chromatography (IMAC) phosphoprotein enrichment and LC-MS, respectively. The results showed that sesamin reduced viability and induced apoptosis in leukemic cells. In addition, 79 phosphoproteins were identified from LC-MS within three main clusters including biological regulation, cellular processand metabolic process. Interestingly, nuclear antigen H731 (PDCD4) and CLIP-associating protein 2 isoform X25 (CLASP2) showed increased in sesamin treated cells and associated protein-ligand interaction network with allicin, capsaicin, cucurbitacin B, and rapamycin which are known to activate apoptosis in cancer cells.Then, sesamin could be developed as candidate of alternative leukemia treatment in the future.


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How to Cite

Pattharin Wannapruk, Atchara Paemanee, Sittiruk Roytrakul, Dalina I Tanyong. Anti-leukemic activity of phosphoproteins from Sesamin via induction of nuclear antigen H731and CLIP-associating protein 2 isoform X25 mediated apoptosis. ijp [Internet]. 2017 Jun. 30 [cited 2023 Nov. 30];9(2):379-88. Available from:



Original Research Articles