Protective role of Broccoli powder against continuous ingestion of Escitalopram antidepressant drug induced hepatotoxicity in Swiss albino male mice

Authors

  • Sneha Saxena THE IIS UNIVERSITY, JAIPUR ICG CAMPUS, GURUKUL MARG, SFS, MANSAROVER, JAIPUR 302020 INDIA
  • Lata Shahani THE IIS UNIVERSITY, JAIPUR ICG CAMPUS, GURUKUL MARG, SFS, MANSAROVER, JAIPUR 302020 INDIA
  • Pradeep Bhatnagar THE IIS UNIVERSITY, JAIPUR ICG CAMPUS, GURUKUL MARG, SFS, MANSAROVER, JAIPUR 302020 INDIA

Keywords:

Escitalopram antidepressant drug, Broccoli powder, CCl4, Hepatotoxicity

Abstract

To investigate the protective role of broccoli powder “Brassica Oleracea Italica” against continuous ingestion of escitalopram antidepressant drug induced hepatotoxicity in Swiss albino male mice. Mice were divided into different groups. Group1: Normal control (0.9% NaCl), Group 2: Escitalopram drug treated only (20 mg/kg), Group 3: Broccoli powder with Escitalopram drug treated (200 mg/kg + 20 mg/kg), Group 4: Olive oil vehicle control, Group 5: Carbon tetrachloride (CCl4) referenced as positive control (33 mg/kg), Group 6: Broccoli powder with CCl4 treated (200 mg/kg + 33 mg/kg). The effect of these groups on liver tissue was studied after three different time periods for 4, 8 and 12 weeks. The results showed that the treatment with escitalopram drug displayed significantly increased serum SGOT, SGPT, ALP level and alter liver antioxidant enzymes level (LPO, SOD and GSH) that are comparable with CCl4intoxicated group considered as positive control. Comparing escitalopram drug treated group with group that received both broccoli powder and escitalopram drug displayed a significant decrease in serum SGOT, SGPT, ALP levels and restored the level of antioxidant enzymes. The protective effect of broccoli powder on escitalopram drug induced hepatotoxicity was also supported by histopathological studies.

References

. Draz EI, Emara AM, Saad KM,

Badaway A, SE HEN, Abd-Elgelil H.

Genotoxicity of some commonly used

antidepressants (fluoxetine, sertraline

and clomipramine). Mansoura J.

Forensic Med. Clin. Toxicol. 2009; 17:

-78.

. Grover S, Avasth A, Kalita K, Dalal PK,

Rao GP, Chadda RK., et al. IPS

multicentric study: Antidepressant

prescription patterns. Indian J.

Psychiatry. 2013; 55: 41-45.

. Wang HY, Chen YC. Escitalopram

intoxication induced acute psychosis.

J. Clin. Toxicol. 2015; 5: 3.

. Khawam EA, Laurencic G, Malone

Jr DA. Side effects of

antidepressants: an overview.

Cleveland Clinic. J. Med. 2006; 73:

-361.

. Ferguson JM. SSRI antidepressant

medications: adverse effects and

tolerability. Prim. Care. Companion J.

Clin. Psychiatry. 2001; 3: 22-27.

. Voican CS, Corruble E, Naveau S,

Perlemuter G. Antidepressant-induced

liver injury: a review for clinicians. AM.

J. Psychiatry. 2014; 171: 404-415.

. Friedrich ME, Akimova E, Huf W,

Konstantinidis A, Papageorgiou K,

Winkler D., et al. Drug-induced liver

injury during antidepressant

treatment: results of AMSP, a drug

surveillance program. Int J

Neuropsychopharmacol. 2016; 19: 1-9.

. Subramanian V. Hepatoprotective

activity of Brassica oleracea italica

against carbon tetrachloride induced in

albino rats. J. Pharm. Res. 2011; 4:

-1144.

. Owis AI. Broccoli; the Green Beauty: A

Review. Pharm. Sci. Res. 2015; 7: 696-

. Cimen B, Gumus CB, Cetin I, Ozsoy

S, Aydin M, Cimen L. The effects of

escitalopram treatment on

oxidative/antioxidative parameters in

patients with depression. Bull. Clin.

Psychopharmacol. 2015; 25: 272-279.

. Hickox WH. Public health goal for

carbon tetrachloride in drinking

water. 2000. OEHHA CalEPA.

Available from: https://oehha.ca.gov/

. El-Baz FK, Salama ZA, Gaafar AA.

Evaluation of hepatoprotective effect of

broccoli Extract against CCL4 in rats.

Int. J. Med. Biol. Front. 2012; 18: 521-

. Food and Drug Administration,

Guidance for industry: estimating the

maximum safe starting dose in initial

clinical trials for therapeutics in adult

healthy volunteers. Center for Drug

Evaluation and Research (CDER)

. Shin JW, Seol IC, Son CG.

Interpretation of animal dose and

human equivalent dose for drug

development. J. Korean Orient. Med.

; 31: 1-7.

. Powell TR, Fernandes C, Schalkwyk

LC. Depression‐Related Behavioral

Tests. Curr. Protoc. Mouse Biol. 2012;

: 119-127.

. Chahardehi AM, Ibrahim D,

Abolhassani F, Sulaiman SF.

Antidepressant-like effects of selected

crude extracts of Pilea microphylla in

mice model of depression. American J.

Agri. Biol. Sci. 2013; 8: 75-81.

. Kumar V, Tomar A, Singh BK,

Nagarajan K, Machawal L, Bajaj U.

Attenuating depression behavior by

Centella asiatica extract & venlafaxine

in mice induced through Forced swim

and Tail suspension test. Int. J.

Pharmacol. Toxicol. 2013; 1: 29-35.

. Chenu F, Guiard BP, Bourin M, Gardier

AM. Antidepressant-like activity of

selective serotonin reuptake inhibitors

combined with a NK1 receptor

antagonist in the mouse forced

swimming test. Behavioural Brain Res.

; 172: 256-263.

. Ohkawa H, Ohishi N, Yagi K. Assay

for lipid peroxides in animal tissues

by thiobarbituric acid reaction. Anal.

Biochem. 1979; 95: 351-358.

. Marklund S, Marklund G.

Involvement of the superoxide anion

radical in the autoxidation of

pyrogallol and a convenient assay for

superoxide dismutase. Eur. J.

Biochem.1974; 47: 469-474.

. Moron MS, Depierre JW, Mannervik B.

Levels of glutathione, glutathione

reductase and glutathione Stransferase activities in rat lung and

liver. Biochimica. ET Biophysica.

Acta.1979; 582: 67-78.

. Abdelmajeed NA. Oxidative tissue

damage induced by citalopram in rat

different organs. Res. J. Medicine Med.

Sci. 2009; 4: 580-586.

. Zlatković J, Todorović N, Tomanović N,

Bošković M, Djordjević S, Lazarević-

Pašti T, et al. Chronic administration of

fluoxetine or clozapine induces

oxidative stress in rat liver: a

histopathological study. Eur. J. Pharm.

Sci. 2014; 59: 20-30.

. Yılmaz A, Elbey B, Yazgan ÜC, Dönder

A, Arslan N, Arslan S, Alabalık U,

Aslanhan H. Protective Effects of

Caffeic Acid Phenethyl Ester on

Fluoxetine-Induced Hepatotoxicity: An

Experimental Study. BioMed research

international. 2016; 1247191.

. Hashem F, Motawea H, ElShabrawy A, El-Sherbini S, Shaker

K, Farrag A.Hepatoprotective activity

of Brassica oleraceae L. var. italica.

Planta Medica. 2013; 12: 177-185.

. Al-Howiriny T. Evaluation of

hepatoprotective activity of broccoli

‘Brassica oleracea’ in rats. Hungarian

Med. J. 2008; 2: 145-156.

. Al-Ghamdi MS. Protective effect of

Nigella sativa seeds against carbon

tetrachlorideinduced liver damage. Am.

J. Chin. Med. 2003; 31: 721-728.

. Eidi A, Mortazavi P, Bazargan M,

Zaringhalam J. Hepatoprotective

activity of cinnamon ethanolic extract

against CCL4-induced liver injury in

rats. EXCLI J. 2012; 11: 495-507.

. Soujanya S, Lakshman M, Kumar AA,

Reddy AG. Evaluation of the protective

role of vitamin C in imidaclopridinduced hepatotoxicity in male Albino

rats. J. Nat. Sci. Biol. Med.2013; 4: 63-

. Cordeiro LC, Kaliwal BB. Protective

role of bark extract of Bridelia retusa

Spreng on CCl4 induced histological

toxicity in mice. J. Pharmacognosy.

Phytochem. 2013; 2: 142-148.

. Althnaian T, Albokhadaim I, El-Bahr

SM. Biochemical and histopathological

study in rats intoxicated with carbon

tetrachloride and treated with camel

milk. Springer plus. 2013; 2:57.

. Abdel SO, Sleem AA, Shafee N. Effect

of trazodone and nefazodone on

hepatic injury induced by carbon

tetrachloride. Drug Discov. Ther. 2010;

: 285-297.

Downloads

Published

30-06-2017

How to Cite

1.
Sneha Saxena, Lata Shahani, Pradeep Bhatnagar. Protective role of Broccoli powder against continuous ingestion of Escitalopram antidepressant drug induced hepatotoxicity in Swiss albino male mice. ijp [Internet]. 2017 Jun. 30 [cited 2024 Oct. 8];9(2):296-304. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/540

Issue

Section

Original Research Articles