Molecular genetic evaluation of Cichorium endivia L. as an anticancer agent against colorectal cancer

Authors

  • Elsayed E Hafez Department of Plant Protection and Biomolecular Diagnosis, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications, Alexandria, Egypt.
  • Effat Badr Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria, Egypt
  • Yasser Mabrouk Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria, Egypt
  • Mohammed El-Seehy Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria, Egypt
  • Sarah Aggag Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria, Egypt

Keywords:

Colorectal cancer, DMH, FU, Cichorium endivia L., PCR, DD

Abstract

Medicinal plants confer protective effects against a wide range of cancers including colon cancer. Cichorium endivia L. has many biological activities and functions, such as anti-inflammation and hepatoprotective effects. This activity is mainly due to its high levels of antioxidant compounds. The effect of cichorium extract and fluorouracil (5-FU) against colorectal cancer (CRC) induced by 1, 2- Dimethyl hydrazine (DMH) was examined in male albino mice. Where, different doses of cichorium extract (200, 400 and 600 mg/kg body weight) were investigated to determine the significant effective dose against colorectal cancer using differential display-PCR (DD-PCR), DNA sequencing, histopathological and cytological techniques. The results showed that the cichorium extract dose 200 mg/kg body weight was the best one compared to the others (400 and 600 mg/kg body weight). This finding could be a powerful therapeutic against colorectal cancer.

References

. Arulraj P, Gopal V, Jeyabalan G.

Digestive Disorder - Colon Cancer -

Emphasising Celecoxib. Int. J. Pharm.

Sci. Rev. Res. , (2015);34, 178-85.

. Perše M, Cerar A. The

dimethylhydrazine induced colorectal

tumours in rat - experimental colorectal

carcinogenesis. Radiol Oncol,

(2005);39, 61-70.

. Longley DB, Harkin DP, Johnston PG.

-Fluorouracil: mechanisms of action

and clinical strategies. Nat Rev Cancer,

(2003);3, 330-8.

. Wang FX, Deng AJ, Li M. (3S)-1,2,3,4-

Tetrahydro-beta-carboline-3-carboxylic

acid from Cichorium endivia. L induces

apoptosis of human colorectal cancer

HCT-8 cells. Molecules, (2012);18,

-29.

. Alshehri A, Hafez E. Molecular and

biochemical evaluation of

antiproliferative effect of (chicorium

endivia, L.) phenolic extracts on breast

cancer cell line: MCF7. E3. Journal of

Biotechnology and Pharmaceutical

Research, (2012);3, 74-82.

. Pan B, Zheng S, Liu C. Suppression of

IGHG1 gene expression by siRNA

leads to growth inhibition and apoptosis

induction in human prostate cancer

cell. Mol Biol Rep, (2013);40, 27-33.

. Sumiyoshi H, Wargovich MJ.

Chemoprevention of 1,2-

dimethylhydrazine-induced colon

cancer in mice by naturally occurring

organosulfur compounds. Cancer Res,

(1990);50, 5084-7.

. Liang P, Pardee AB. Differential display

of eukaryotic messenger RNA by

means of the polymerase chain

reaction. Science, (1992);257, 967-71.

. Seufi MA, Galal FH, Hafez EE.

Characterization of a Schistocerca

gregaria cDNA Encoding a Novel

Member of Mobile Phone RadiationInduced Polypeptide Related to

Chitinase Polypeptide Family. Journal

of Applied Sciences Research,

(2007);3, 733-740.

. Liu D, Yin B, Wang Q. Cytoplasmic

poly(A) binding protein 4 is highly

expressed in human colorectal cancer

and correlates with better prognosis. J

Genet Genomics, (2012)39, 369-74.

. Nady M E, Mansour A M, Hafez E E,

Omran G, Hamad G M, Harraz S E,

Allam S N, Ahamad A A. Chicory

abrogates oxidative stress,

inflammation and caspase-dependent

apoptosis in acute hepatic injury model

induced by acetaminophen in rats.

International Journal of Phytomedicine

(2016).

. Pan H, Yan BS, Rojas M. Ipr1 gene

mediates innate immunity to

tuberculosis. Nature, (2005);434, 767-

. Matsuoka H, Obama H, Kelly ML.

Biphasic functions of the kinasedefective Ephb6 receptor in cell

adhesion and migration. J Biol Chem,

(2005);280, 29355-63.

. Kiyokawa E, Takai S, Tanaka M.

Overexpression of ERK, an EPH family

receptor protein tyrosine kinase, in

various human tumors. Cancer Res,

(1994)54, 3645-50.

. Young SZ, Bordey A. GABA's control of

stem and cancer cell proliferation in

adult neural and peripheral niches.

Physiology (Bethesda), (2009);24, 171-

. Liu J, Zheng Q, Deng Y. A seven-helix

coiled coil. Proceedings of the National

Academy of Sciences, (2006);103,

-62.

. Korzeniewski N, Hohenfellner M,

Duensing S. CAND1 promotes PLK4-

mediated centriole overduplication and

is frequently disrupted in prostate

cancer. Neoplasia, (2012)14, 799-806.

. Alshehri A . Molecular and Biochemical

Evaluation of Anti-Proliferative Effect of

(Cichorium endivia L.) Phenolic

Extraction Cancer Cell Line HCT-116.

Academic Journal of Cancer Research,

(2012);5, 53-60.

. Hafez EE, Badr EA, Mabrouk YM.

Expression of tumor-markers and

cytokines in response to cichorium

endivia l. In cancerous mice. Int J

LifeSc Bt & Pharm Res, (2014);3, 33-

Downloads

Published

31-12-2016

How to Cite

1.
Elsayed E Hafez, Effat Badr, Yasser Mabrouk, Mohammed El-Seehy, Sarah Aggag. Molecular genetic evaluation of Cichorium endivia L. as an anticancer agent against colorectal cancer. ijp [Internet]. 2016 Dec. 31 [cited 2024 Dec. 7];8(4):551-7. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/503

Issue

Section

Original Research Articles