Evaluation of anti-hyperglycemic and anti-hyperlipidemic effects of Naravelia zeylanica in streptozotocin- induced diabetic rats
Keywords:
Anti-hyperglycemic, Glibenclamide, Naravelia zeylanica, streptozotocin, RanunculaceaeAbstract
Naravelia zeylanica DC (Ranunculaceae), a woody climber, have been used from ancient times to treat various ailments like rheumatoid arthritis, skin diseases, wound and ulcer. The present study was designed to investigate the anti-hyperglycemic activity of methanolic extract of Naravelia zeylanica (NZYM) using experimental diabetic model. Diabetes was induced in wistar rats by a single dose of streptozotocin (55 mg/kg i.p.) (STZ) and treated with NZYM at doses of 100 and 200 mg/kg for 45 days. Glibenclamide (5 mg/kg b.w.) was used as standard drug. Blood glucose and body weight were monitored at regular intervals and the levels of serum insulin, lipid and the carbohydrate metabolic enzyme in the liver were measured at the end of the study. Oral administration of NZYM and glibenclamide significantly reduced the blood glucose level (p<0.05), with increased serum insulin and significant alteration in lipid profiles and liver carbohydrate enzymes (p<0.05) after 45 days. Furthermore, the biochemical parameters correlated with the histopathological changes in the pancreas of STZ-induced diabetic rats, which structurally proved the efficacy of NZYM. The findings suggest that NZYM possess anti-hyperglycemic activity and anti-hyperlipidemic properties and restored STZ-induced pancreatic damage in diabetic rats. NZYM might therefore have a beneficial effect in treatment of diabetes mediated through regulation of carbohydrate metabolic enzyme activities.
References
Li WL, Zheng HC, Bukuru J, De Kimpe N. Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus. J Ethnopharmacol. 2004; 92 (1):1-21.
Prout TE. Excerpta Medica. In Proceedings VIII Congress of International Diabetes Federation, Eds WJ Malaisse, J Pirart. Amsterdam: Elsevier Publishing. 1974; 162.
Hu X, Sato J, Oshida Y, Xu M, Bajotto G, Sato Y. Effect of Goshajinki-Gan (Chinese herbal medicine: Niu-che-senQi-wan) on insulin resistance in streptozotocin induced diabetic rats. Diabetes Res Clin Pract. 2003; 59(2): 103 –111.
Ahuja J, Suresh J, Deep, Madhuri, Partyusha, Ravi. Phytochemical Screening of Aerial Parts of Artemisia parviflora Roxb; A medicinal plant. Der Pharmacia Letter. 2011: 3(6):116-124.
Uma C, Suganya N, Vanitha P, Bhakkiyalakshmi E, Suriyanarayanan S, Maria John KM, Sivasubramanian S, Gunasekaran P and Ramkumar KM. Antihyperglycemic effect of Codariocalyx motorius modulated carbohydrate metabolic enzyme activities in streptozotocin-induced diabetic rats. J Fun Foods. 2014; 11: 517-527.
Kumar S, Narwal S, Kumar D, Singh G, Sumit Narwal, Arya R. Evaluation of antihyperglycemic and antioxidant activities of Saraca asoca (Roxb.) De Wild leaves in streptozotocin-induced diabetic rats. Asia Pac J Trop Disease. 2012; 2(3): 170-176.
Zheng X, Yu-jie Li, Li Zhang, Wei-sheng Feng, XinZhang. Antihyperglycemic activity of Selaginella tamariscina (Beauv.) Spring. J Ethnopharmacol. 2011; 133(2): 531-537.
Latha M, Pari L, Sitasawad S, Bhonde R. Insulin-secretagogue activity and cytoprotective role of the traditional antidiabetic plant Scoparia dulcis (Sweet Broomweed). Life Sci. 2004; 75(16): 2003-14.
Ramkumar KM, Vanitha P, Uma C, Suganya N, Bhakkiyalakshmi E, Sujatha J. Antidiabetic activity of alcoholic stem extract of Gymnema montanum in streptozotocin-induced diabetic rats. Food Chem Toxicol. 2011; 49(12): 3390-4.
Rajendran J, Jyothi A, Jacob L, Vinod S, Kochupappy RT. Simultaneous determination of berberine and β- sitosterol in the leaf extracts of Naravelia zeylanica by analytical methods and their in vitro Anti diabetic activity. J Adv Pharm Edu Res. 2014; 4(3): 365-371
Jaroszewski JW, Staerk D, Holm-Moller SB, Jensen TH, Franzyk H, Somanadhan B. Naravelia zeylanica [corrected] occurrence of primary benzamides in flowering plants. Nat Prod Res. 2005; 19(3): 291-4.
Shenoy, Ashoka, M,Shastry, CS,Sridevi, Gopkumar P. Anti-Ulcer Activity of Naravelia Zeylanica Leaves Extract. J Pharm Res. 2009; 2(7): 1218-1220.
Uvarani M, Sutharsingh R, William Arputha Sundar AS, A. Thangathirupathi. Evaluation of antibacterial activity of aerial parts of Naravelia zeylenica. Int J Pharm Biomed Sci. 2012; 3(2): 792-796.
Sutharsingh R, Kavimani S, B.Jayakar, Uvarani M, Thangathirupathi A. Quantitative phytochemical estimation and antioxidant studies on aerial parts of Naravelia Zeylanica. Int J Pharm Sci Drug Res. 2011; 2(2): 52-56.
Harbone, J B. Methods of Extraction and isolation In: Phytochemical methods, Chapman and Hall, London, 1991; 60-66.
Sekar N, Kanthasamy S, William S, Subramanium S, Govindasamy S. Insulinic action of vanadate in diabetic rats. Pharmacol Res. 1990; 22(2): 207-17.
Ramachandran S, Asokkumar K, Uma Maheswari M, Ravi TK, Sivashanmugam AT, Saravanan S, Rajasekaran A, Dharman J. Investigation of antidiabetic, antihyperlipidemic, and in vivo antioxidant properties of Sphaeranthus indicus Linn.in type 1 diabetic rats: An identification of possible biomarkers. Evid Based Complement Alternate Med. 2010; 24-31.
Kumar A, Ilavarasan R, Jayachandran T, Deecaraman M, Aravindan P, Padmanabhan N, Krishan MR. Anti-diabetic activity of Syzygium cumini and its isolated compound against streptozotocin-induced diabetic rats. J Med Plant Res. 2008; 2(9): 246-249.
Hossain MS, Ahmed M, Islam A; Hypolipidemic and hepatoprotective effects of different fractions of methanolic extract of Momoridica charantia (Linn.) in alloxan induced diabetic rats. Int J Pharma Sci Res. 2011; 2(3): 601-607.
Chikhi I, Allali1 H, El Amine Dib M, Medjdoub H, Tabti B. Antidiabetic activity of aqueous leaf extract of Atriplex halimus L (Chenopodiaceae) in streptozotocin-induced diabetic rats. Asian Pac J Trop Dis. 2014; 4(3): 181–184.
Brandstrup N, Kirk JE, Bruni C, The hexokinase and phosphoglucoisomerase activities of aortic and pulmonary artery tissue in individuals of various ages. J Gerontol, 1957; 12(2): 166-171.
Zak B, Epstein E, Baginski ES. Determination of liver microsomal glucose-6-phosphatase. Ann Clin Lab Sci, 1997; 7(2): 169-177.
Ells HA, & Kirkman HN. A colorimetric method for assay of erythrocytic glucose-6-phosphate dehydrogenase. Proc Soc Exp Biol Med. (New York, N.Y.), 1961; 106(3): 607-609.
Huijing, F. A rapid enzymic method for glycogen estimation in very small tissue samples. Clinica Chimica Acta. 1970; 30(3): 567–572.
Bansal R, Ahmad N, Kidwai JR. Alloxan-glucose interaction:effect on incorporation of 14C-leucine into pancreatic islets of rat. Acta Diabetol Lat. 1980; 17: 135–143.
Tanko Y, Yerima M, Mahdi MA, Yaro AH, Musa KY, Mohammed A. Hypoglycemic activity of methanolic stem bark Adasonnia digitata extract on blood glucose levels of streptozocin-induced diabetic wistar rats. Int J Appl Res Nat Prod. 2008; 1(2): 34-36.
Hazra M, Kundsen S, Bhattacharya S, Haldar KP, Gupta M, Mazumder KU. Evaluation of hypoglycemic and antihyperglycemic effects of Luffa cylindrica fruit extract in rats. J Adv Pharma Edu Res. 2011; 2: 138-146.
Andrade Cetto, A., Wiedenfeld, H., Revilla,MC., Serigo, IA. Hypoglycemic effect of Equisetum myriochoetum aerial parts on streptozotocin-diabetic rats. J Ethanopharmacol. 2000; 72(1-2): 129-133.
Baquer NZ, Gupta D, Raju J. Regulation of metabolic pathways in liver and kidney during experimental diabetes: Effects of antidiabetic compounds. Indian J Clin Biochem. 1998; 13: 63-80.
Ananda PK, Kumarappan CT, Sunil C, Kalaichelvan VK. Effect of Biophytum sensitivum on streptozotocin and nicotinamide-induced diabetic rats. Asian Pac J Trop Biomed. 2012; 2(1): 31-5.
Punitha IS, Rajendran K, Shirwaikar A, Shirwaikar A. Alcoholic stem extract of Coscinium fenestratum regulates carbohydrate metabolism and improves antioxidant status in streptozotocin-nicotinamide induced diabetic rats. Evid Based Complement Alternat Med. 2005; 2(3): 375-81.
Juarez-Rojop IE, Diaz-Zagoya JC, Ble-Castillo JL, Miranda-Osorio PH, Castell-Rodriguez AE, Tovilla-Zarate CA, Rodríguez-Hernández A, Aguilar-Mariscal H, Ramón-Frías T and Bermúdez-Ocaña DY. Hypoglycemic effect of Carica papaya leaves in streptozotocin-induced diabetic rats. BMC Complement Altern Med. 2012; 12: 236.
Jothivel N, Ponnusamy SP, Appachi M, Singaravel S, Rasalingam D, Deivasigamani K, Thangavel S. Anti-diabetic activity of methanolic extract of Costus pictus D.Don in Alloxan – induced diabetic rats. J Health Sci. 2007; 53(6): 655-663.
Manasa Barlanka and Venu Gopal Y. Naravelia zeylanica: a review. Int J Pharm. 2013; 3(1): 241-246. 35. Yin J, Ye J, Jia W. Effects and mechanisms of berberine in diabetes treatment. Acta Pharmaceutica Sinica B. 2012; 2(4): 327–334.
