Anti-diabetic effect of aqueous fruit extract of Borassus aethiopum (Mart.) in alloxan-induced diabetic rats

Authors

  • Christopher Larbie ORCID iD Kwame Nkrumah University of Science and Technology Ghana.
  • Jeremiah Issaka Department of Biochemistry and Biotechnology, KNUST, Kumasi Ghana.
  • Marina Tandoh Department of Biochemistry and Biotechnology, KNUST, Kumasi Ghana.

Keywords:

Borassus aethiopum, Alloxan, Diabetes, hepatoprotection

Abstract

Introduction: Diabetes mellitus (DM) is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The chronic hyperglycemia is associated with long-term damage, dysfunction, and failure of various organs, especially the eyes, kidneys, nerves, heart, and blood vessels and is among the top ten causes of death in the world. Borassus aethiopum Mart. (family Arecaceae) is a plant species of Borassus palm found widely across Africa. It serves an important source of food, providing edible fruits, and nuts, and also has a number of pharmacological uses that have been reported in some parts of the world. Objective: The current study was aimed at assessing the phytochemical constituents as well as the antidiabetic and hypolipidaemic effect of fruit extract of B. aethiopum in alloxan-induced diabetic rats. Materials and Methods: The fruits extract was prepared (FEB) and phytochemical constituents evaluated using standard methods. The antidiabetic and hypolipidaemic properties in alloxanized rats assessed for 7 and 28 days. Normoglycaemic and alloxan-induced diabetic rats were treated with FEB at doses of 100 mg, 250 mg and 500 mg/kg body weight. Observations on body weight, relative organ weight, haematological and biochemical parameters were measured in both acute and sub-chronic circumstances. Results: The presence of tannins, saponins, glycosides, triterpenoids and alkaloids were detected. Fasting blood glucose was reduced significantly (p<0.05) in diabetic rats in acute study at a dose of 500 mg/kg body weight and at 250 mg and 500 mg/kg body weight in sub-chronic studies. WBC and PLT levels were significantly increased after treatment with 500 mg/kg body weight. Urea and ALT levels also reduced significantly in both acute and sub-chronic studies. Conclusion: The aqueous fruit extract of B. aethiopum is antidiabetic. It was also found to be nephron- and hepato-protective as well as boosting the immunity of the animals.

References

. Kaku K. Pathophysiology of Type 2 Diabetes and its treatment policy.Japan Medical Association Journal, 2010; 53(1): 41–46.

. International Diabetes Federation. IDF Diabetes Atlas Sixth Edition. 2014.

. World Health Organisation. Global status report on noncommunicable disease, 2011.

. Thévenod F. Pathophysiology of diabetes mellitus type 2: Roles of obesity, insulin resistance and β-cell dysfunction. Front Diabetes. Basel, Karger, 2008;19:1–18.

. World Health Organisation. Management of diabetes mellitus. Standards of care and clinical practice guidelines. 1994.

. Okeke EC. The use and chemical content of some indigenous Nigerian spices. Journal of Herbs, Spices and Medicinal Plants 1998;5(4):51-63.

. WHO. The World Health Report 2008.

. Haynes J and McLaughlin J. Edible palms and their uses. Florida, USA: University of Florida, Institute of Food and Agricultural Sciences 2000.

. Agbo NG and Simard RE. Characteristics of juice from palmyrah palm (Borassus) fruit. Plant Foods For Human Nutrition, 1992; 42(1):55-70.

. Mshana N. R, Abbiw DK, Addae-Mensah I, Adjanohoun E, Ahyi M R, Enow- Orock EG, Gbile Z O, Noamesi BK, Odei M A, Adunlami H, Oteng –Yeboah AA, Sarpong K, Sofowora A, Tackie A N. Traditional medicine and pharmacopoeia.: Contribution to the revision of ethnobotanical and floristic studies in Ghana. OAU/STRC 2000.

. Amoateng P, Kumah DB and Koffuor G. Antioxidant and free radical scavenging properties of an aqueous ripe fruit extract of Borassus aethiopum. West African Journal of Pharmacology, 2010; 26:8-14.

. Koffi E K, Ezoua P, Sidibe D and Agbo NG. Sensory analysis of the fruit juice of palmyrah palm (Borassus aethiopum): a decision making tool. African Journal of Food Agriculture Nutrition and Development, 2010;10(7): 2818-2833

. Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis 3rd edition. Chapman and Hall, London, 1998. 235.

. Trease GE and Evans WC. Pharmacognosy, 12th Edition, Balliere-Tindall, London. 1989; 241 – 260.

. Sofowora A. Phytochemical screening of medicinal plants and traditional medicine in Africa. 2nd Edition Spectrum Books Limited, Nigeria, 1993;150 – 156

. Joy KL and Kuttan R. Antidiabetic activity of Picrorrhiza kurrroa extract. Journal of Ethnopharmacology, 1999; 67:143-8.

. Bennett W L, Maruthur N M , Singh S, Segal J B, Wilson L M, Chatterjee R, Marinopoulous S S, Puhan M A, Ranasinghe P, Block L, Nicholson WK, Hutfless S, Bass E B and Bolen S. Comparative effectiveness and safety of medications for type 2 diabetes: an update including new drugs and 2-drug combinations. Annals of Internal Medicine, 2011: 154(9):602-613.

. Pradeep G, Anil K A, Lakash MS and Singh GK. Antidiabetic and antihyperlipidemic effect of Borassus flabellifer in streptozotocin (STZ) induced diabetic rats. World Journal of Pharmacy and Pharmaceutical Sciences 2015;4(1):1172-1184.

. Sarkodie AJ, Squire AS, Kretchy A I, Bekoe OE, Domozoro YFC, Ahiagbe M JK, Adjei E, Edoh AD, Amponsah K I, Sakyiama M, Lamptey K V, Affedzi-Obresi S, Duncan L J, Debrah P, N’guessan BB, and Nyarko K. A Borassus aethiopum, A Potential Medicinal Source of Antioxidants, Anti-Inflammatory and Antimicrobial Agents. Herbal Medicine. 2015;1: 1-3

. Okonta J M and Aguwa C N. Evaluation of hypoglycaemic activity of glycosides and alkaloids extracts of Picralima nitida Stapf (Apocynaceae) seeds. International Journal of Pharmacolology, 2007; 3(6):505 – 509

. Ali A, Alhadji D, Tchiegang C and Saïdou C. Physico-chemical properties of palmyra palm (Borassus aethiopum Mart.) fruits from Northern Cameroon. African Journal of Food Science, 2010;4(3): 115-119.

. Yorshikawa M, Murakami T, Matsuda H, Yamahara J, Murakami N and Kitagawa I. Bioactiye Saponins and Glycosides.III. Horse Chestnut. (1): The Structures, Inhibitory Effects on Ethanol Absorption, and Hypoglycemic Actiyity of EscinsIa, Ib, IIa, IIb, and IIIa from the Seeds of Aescultts hippocastanum L. Pharmaceutical Society of Japan Bulletin 1996; 44(8):1454-1464

. Soetan K O, Akinrinde A S and Ajibade TO. Preliminary studies on the haematological parameters of cockerels fed raw and processed guinea corn (Sorghum bicolor) Proceedings of 38th Annual Conference of Nigerian Society for Animal Production. 2013;49-52.

. Isaac LJ, Abah G, Akpan B and Ekaette, IU. Haematological properties of different breeds and sexes of rabbits Proceedings of the 18th Annual Conference of Animal Science Association of Nigeria 2013;.24-27.

. Purves WK, Sadava D, Orians G H and Heller HC. Life: The science of Biology (7th Ed.). Sinauer Associates and W. H. Freeman. 2003;954

. Crook M. A Clinical chemistry and metabolic medicine.7th edition.Hodder A rnold, London. 2006; 426.

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Published

30-09-2016

How to Cite

1.
Christopher Larbie, Jeremiah Issaka, Marina Tandoh. Anti-diabetic effect of aqueous fruit extract of Borassus aethiopum (Mart.) in alloxan-induced diabetic rats. ijp [Internet]. 2016 Sep. 30 [cited 2024 Dec. 7];8(3):384-97. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/483

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