Evaluation of central nervous system depressant activity of methanolic and petroleum ether extract of Manilkara zapota leaves (in vivo)


  • Manirujjaman Southeast University, Bangladesh .
  • Trudi Collet Queensland University of Technology. Australia


CNS depressant, hole cross open field, Manilkara zapota leaves


The purpose of this study was to examine the central nervous system (CNS) -depressant activity of methanolic and petroleum ether extracts of leaves obtained from Manilkara zapota (Sapotaceae) in vivo. CNS-depressant action was evaluated by observing the effects of plant extracts on both exploratory and spontaneous locomotor activity in mice using open field and hole cross tests respectively. The extracts were found to exhibit CNS-depressant activity in a dose-dependent manner. In the open field test, both the methanolic and petroleum ether extracts reduced the exploratory behavior of mice significantly (p<0.05). Although the extracts reduced locomotor activity in the hole cross test, the results were found to be insignificant. Overall, these findings indicate that both types of extracts derived from M. zapota leaves possess CNS-depressant activity.


Islam MR, Parvin MS, Islam MS, Hasan SMR, Islam ME. Antioxidant Activity of the Ethanol Extract of Manilkara zapota Leaf. J Sci Res.2012; 4: 193-202. 2. Manirujjaman, Sultana F, Chowdhury MAR, Shimu MC, Hossain MT, Haque MIU. In Vivo assay of Antidiarrhoeal activity of Methanolic and Petroleum ether extracts of Manilkara Zapota Leaves. Int. J. Drug Dev. & Res. 2013; 5 (4): 164-171. 3. Hossain H, Howlader MSI, Dey SK, Hira A, Ahmed A. Antinociceptive and antidiarrheal properties of the ethanolic extract of Manilkara zapota (Linn.) Bark. Int J Pharm Sci Res. 2012; 3: 4791-4795. 4. Mohiddin HMYB, Chin W, Holdsworth D. Traditional medicinal plants of Brunei, Darussalam Part III. Sengkurong. Int J Pharmacog. 1992; 30: 105-108. 5. Chanda SV, Nagani KV. Antioxidant Capacity of Manilkara zapota Leaves Extracts Evaluated by Four in vitro Methods. Nat and Sci. 2010; 8: 797-802. 6. Nair R, Sumitra C. Antimicrobial Activity of Terminalia catappa, Manilkara zapota and Piper betel Leaf Extract. Indian J Pharm Sci. 2008; 70: 390–393. 7. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983; 16: 109. 8. Gupta BD, Dandiya PC, Gupta ML. A psychopharmacological analysis of behavior in rat. Jpn J Pharmacol. 1971; 21: 293. 9. Takagi K,Watanbe M, Satio H. Studies on the spontaneous moment of animals by the hole cross test. Jpn J Pharmacol. 1971; 2: 797. 10. Rakotonirina VS, Bum EN, Rakotonirena A, Bopelet M. Sedative properties of the decoction of the rhizom of Cyperus anticulatives. Fitoterapia. 2001; 72: 22-29. 11. Dorr M, Joycee D, Porsolt RD, Steinberg H, Summerfield A, Tomkiewiez M. Persistence of dose-related behaviour in mice. Nature.1971; 231:121-123. 12. Mansur RM, Martz W, Carlini EA. Effects of acute and chronic administration of Cannabis satis and (-) 9-trans tetrahydro cannabinaol on the behaviour of rats in open field arena. Psychopharmacol. 1980; 2: 5-7. 13. Ozturk Y, Aydini S, B¬eis R, Baser KHC, Berberoglu H. Effect of Hypericum pericum L. and Hypericum calycinum L. extracts on the central nervous system in mice. Phytomed.1996; 3: 139-146. 14. Nagarjun NS, Soundari PG, Kumaresan PT. CNS depressant activity of Dalbergia malaberica. Indian Drugs. 2003; 40: 716–717. 15. Ali MS, Nasrin M, Dash PR. Evaluation of Analgesic and CNS Depressant Activities of Grewia Paniculata in Swiss albino Mice. A J Food and Nutrition. 2015; 3 (1): 21-27. 16. Kolawole OT, Makinde JM, Olajide OA 2007. Central nervous depressant activity of Russelia equisetiformis. N J Phy Sci. 2007; 22: 59-63.




How to Cite

Manirujjaman, Trudi Collet. Evaluation of central nervous system depressant activity of methanolic and petroleum ether extract of Manilkara zapota leaves (in vivo). ijp [Internet]. 2016 Sep. 30 [cited 2023 Nov. 30];8(3):308-11. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/472



Original Research Articles