Hepatoprotective Activity of Aqueous Extract of Sesbania grandiflora Linn Leaves against Carbon Tetrachloride induced Hepatotoxicity in Albino Rats

Authors

  • Dayananda Bhoumik Department of Pharmacy, College of Health Sciences Mekelle University, Mekelle, Ethiopia
  • Arunabha Mallik BANSAL COLLEGE OF PHARMACY,BHOPAL
  • Abera Hadgu Berhe Department of Pharmacy, College of Health Sciences Mekelle University, Mekelle, Ethiopia

Keywords:

Hepatoprotective effect, Histopathological Studies, Biochemical Parameter, Carbon Tetrachloride, Sesbania grandiflora

Abstract

The present study was conducted to evaluate the hepatoprotective activity of aqueous extract of leaves of Sesbania grandiflora Linn (AESG) at the dose of 250 and 500 mg/kg body weight per oral using Carbon tetrachloride induced liver damage in wistar albino rats. Aqueous extract showed significant (p<0.05) hepatoprotective effect by lowering the serum levels of various biochemical parameters such as serum glutamic oxaloacetate transaminase (SGOT), serum glutamic pyruvates transaminase (SGPT), alkaline phospatase (ALP), total bilirubin (TBL), total cholesterol (CHL) and by increasing the levels of total protein (TPTN) and albumin (ALB), in the selected model. These biochemical observations were inturn confirmed by histopathological examinations of liver sections and were comparable with the standard hepatoprotective drug Silymarin (100 mg/kg body weight i.p.) which served as a reference control. It was concluded from the result that the aqueous extract of Sesbania grandiflora L. possesses hepatoprotective activity against Carbon tetrachloride induced hepatotoxicity in rats.

References

. Arias PS, Bessey AA, Lowery DM and Brock M. Lipid peroxidation Biochemistry measurement and significance in liver cell injury. Indian. J. Exp. Biol., 1989;45: 87-89.

. Micheale P, Cynthiya Ju. Mechanism of Drug induced liver injury. The AAPS Journal.2006;1: 48-54.

. Cpherson, G. E. 1921. Comparison of development in dodder & morning glorry. Botanical glazette, 71, 392-398.

. Kirthikar KR, Basu BD. Indian Medicinal Plants, Vol III. Dehradun: Bishen Singh Mahendra Pal Singh, 1998: 735-736.

. Asima Chatterjee, Satyesh Chandra Pakrashi. The Treatise on Indian Medicinal Plants, Vol II. New Delhi: Publication and Information Directorate, 1992: 118.

. Prajapati, Purohit. A Handbook of Medicinal Plant A to Z. section II, 473.

. Rastogi RP. Compendium of Indian Medicinal Plants. Vol. I, 1960: 371.

. Das KC, Tripati AK. A new flavanol glycoside from Sesbania grandiflora (Linn). Fitoterapia 1998;69(5):477-478.

. Warrier PK, Nambiar VPK, Ramankutty C. Indian Medicinal Plant, vol 5. Madras: Oriental Longman Ltd, 1996: 116-117.

. Nadkarni AK, Indian Materia Medica. Bombay Popular Prakashan, 2nd ed, 2009: 52-54.

. Sreelatha S, Padma PR, et al. Evaluation of anticancer activity of ethanol extract of Sesbania grandiflora (Agati Sesban) against Ehrlich ascites carcinoma in Swiss albino mice. J Ethnopharmacol 2011; 134(1):984-987.

. Ramesh T, Sureka C, et al. Sesbania grandiflora diminishes oxidative stress and ameliorates antioxidant capacity in liver and kidney of rats exposed to cigarette smoke. J Physiol Pharmacol 2010; 61(4):467-476.

. Ramesh T, Mahesh R, et al. Cardioprotective effects of Sesbania grandiflora in cigarette smoke-exposed rats. J Cardiovasc Pharmacol 2008; 52(4):338-343.

. Doddola S, Pasupulati H. Evaluation of Sesbania grandiflora for antiurolithiatic and antioxidant properties. J Nat Med 2008; 62(3):300-307.

. Pari L, Uma A. Protective effect of Sesbania grandiflora against erythromycin estolate-induced hepatotoxicity. Therapie 2003; 58(5):439-443.

. Kasture VS, Deshmukh VK. Anxiolytic and anticonvulsive activity of Sesbania grandiflora leaves in experimental animals. Phytotherapy Research. 2002; 16(5):455-460.

. P Karthikeyan, V Suresh, A Suresh. Wound healing activity of Sesbania grandiflora (L.) poir. Bark. Int J of Pharm. Research & Development 2011; 3(2): 87-93.

. Sunil S. Jalalpurea, Kallanagoda R. Alagawadia, Channabasappa S. Mahajanshetty. In Vitro Anthelmintic Property of Various Seed Oils. Iranian J of Pharmaceutical Research 2006; 4: 281-284.

. Kokate CK. Practical Pharmacognosy, 4th edition, New Delhi: Vallabh Prakashan; 1999: 149-156.

. Khandelwal KR. Practical Pharmacognosy techniques and experiments. 2nd edition, Pune: Nirali Prakashan; 2000: 149-156.

. Ecobichon, DJ. The basis of toxicology testing. 2nd edn, Newyork, CRC press, 1999; 43-46.

. Gupta M, Mazumder UK, Kumar TS., Gomathi P, Kumar RS. Antioxidant and hepatoprotective effects of Bauhinia racemosa against paracetamol and carbon tetrachloride induced liver damage in rats. Iranian J Pharmacology and Therapeutics 2004; 3:12-20.

. Mc Comb RB, Bowers GN. Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum. Clin Chem., 1972; 18(2):97-104.

. Jendrassik L, Grof P. Quantitative determination of total and direct bilirubin in serum and plasma. Biochem., 1938; 297:81-9.

. Richmond W. Preparation and properties of a cholesterol oxidase from Nocardia species and its application to the enzymatic assay of total cholesterol in serum. Clin Chem., 1973; 19(12):1350-6.

. Peters T. Proposals for standardisation of total protein assays. Clin Chem., 1968; 14:1147-59.

. Webster D. A study of intraction of bromocresol green with isolated serum globulin fractions. Clin Chim Acta., 1974; 53:109-15.

. Galighor AE, Kozloff EN. Essentials of practical microtechnique 2nd edn, Lea and Febiger, NewYork, 1976.

. Dunnet CW. New tables for multiple comparisions with a control. Biometrics, 1964; 20:482 -91.

. Venukumar MR, Latha MS. Hepato protective effect of the methanolic extract of orchids in Carbon tetrachloride treated male rats. J. Pharmacol., 2002; 34:2.

. Recknagel RO, Glender EA, Walter RL. Mechanism of Carbon tetrachloride toxicity. Pharmal ther., 1989; 43:139-54.

. Farooq S, Ahmed I, Pathak GK. In protective role of koflet (an Ayurvedic preparation) against cellular toxicity caused by Carbon tetrachloride and flyash. J Ethnopharmacol., 1997; 53:109-116.

. Mitra SK, Venkataranganna MV, Sundaram R, Gopumadhavan S. Protective effects of HD-03, A herbal formulation, against various hepatotoxic agents in rats. Ethnopharmacol J., 1998; 63:181-86.

Downloads

Published

30-06-2016

How to Cite

1.
Dayananda Bhoumik, Arunabha Mallik, Abera Hadgu Berhe. Hepatoprotective Activity of Aqueous Extract of Sesbania grandiflora Linn Leaves against Carbon Tetrachloride induced Hepatotoxicity in Albino Rats. ijp [Internet]. 2016 Jun. 30 [cited 2024 Dec. 5];8(2):294-9. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/470

Issue

Section

Original Research Articles