The Protective Effects of Curcuminand Caffeic acid alone or in combination onNicotine-induced Lung Injury in Rats


  • Sylvia A. Boshra Biochemistry Department, Faculty of Pharmacy, October 6 University, 6th of October City, Egypt


Nicotine, lung, curcumin, caffeic acid, N-acetylcysteine, oxidative stress biomarkers


The present study was performed to explore the protective effects ofcaffeic acid (20 mg/ and curcumin (50mg/k.g.b.w.) on nicotine-induced lung injury alone and in combination.Their effect was compared to N-acetylcysteine (500mg/k.g.b.w.) as known modulator of oxidative stress. Nicotine treatment (0.6mg/kg/day, i.p, for 21 consecutivedays) resulted in a significantincrease (p<0.05) in plasmaalanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C) and well as plasma and lung thiobarbituric acid reactive substances (TBARS), nitric oxide(NO) and tumor necroses factor-α (TNF-α) concomitant with significant decline in non-enzymatic antioxidant like reduced glutathione(GSH) and in enzymatic antioxidants like catalase (CAT) andsuperoxide dismutase (SOD) as well as high density lipoprotein cholesterol (HDL-C). Furthermore, nicotine treatment caused severe injury indicated bythe histopathological examination of lung tissue compared to normal control group. Oral treatment with caffeic acid alone orcurcuminalone or in combination as well as N-acetylcysteine alone prevented the elevation in plasma ALT, AST, LDH,TC, TG, LDL-C, NO, TNF-α and TBARSlevels concomitant with an increments in the HDL-C, reduced glutathione GSH and antioxidant enzymes (CAT and SOD) and amelioration inhistopathological changes and injury induced by nicotine. Lung protection was prominent in curcuminand N-acetylcysteine alone more than caffeic acid alone or caffeic acid and curcuminin combination. Moreover, curcumin has the potential to be used in a combination therapy with caffeic acid, with decreasing the therapeutic dose of caffeic acid and therefore its side-effects.


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How to Cite

Sylvia A. Boshra. The Protective Effects of Curcuminand Caffeic acid alone or in combination onNicotine-induced Lung Injury in Rats. ijp [Internet]. 2016 Jun. 30 [cited 2023 Nov. 29];8(2):238-4. Available from:



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