Antimicrobial Activity of Some Trigonella Species

Authors

  • Rakhee Shyam Dangi Assistant Professor India Cell andMolecular Biology.
  • Dasharath Oulkar National research Centre for Grapes, Solapur road Pune Maharashtra India.
  • Prashant Dhakephalkar National research Centre for Grapes, Solapur road Pune Maharashtra India.
  • Sanjay Kumar Singh National research Centre for Grapes, Solapur road Pune Maharashtra India.
  • Kaushik Banerjee National research Centre for Grapes, Solapur road Pune Maharashtra India.
  • Dattatray Naik National research Centre for Grapes, Solapur road Pune Maharashtra India.
  • Shubhada Tamhankar National research Centre for Grapes, Solapur road Pune Maharashtra India.
  • Suryaprakasa Rao National research Centre for Grapes, Solapur road Pune Maharashtra India.

Keywords:

Antimicrobial activity;, Trigonella suavissima Lindl, T. spicata Sm, Saponins, Sapogenin

Abstract

The genus Trigonella includes many medicinal and aromatic plant species used in traditional as well as veterinary medicines for different diseases, alone or in combination with other remedies. The crude methanol extracts of 15 Trigonella species were assayed for antimicrobial activity against four medicinally important multidrug resistant clinical isolates, five plant pathogenic bacteria and five fungi. Three species showed a broad spectrum of antibacterial activity inhibiting all the test bacteria. Acidified ethanolic extract of the most promising species, T. suavissima was purified by column chromatography. Characterization of a partially purified ethyl acetate fraction by LC/ESI/MS showed the presence of 7’4’ dihydroxy flavone and (2E)-3-{5-[4-(Ethoxycarbonyl)phenyl]furan-2-yl}prop-2-enoate) Three species showed strong antifungal activity against Aspergillus niger and Fusarium solani. The saponin extract of T. spicata showed a selective activity against A. niger and the sapogenin extract against F. solani. A polyhydroxylated alkaloid was isolated from the sapogenin extract of T. spicata using chromatographic techniques with structural characterization done by LC/ESI/MS, 13C and 1H NMR. The study reveals that Trigonella species are potential sources of natural compounds that may act as antimicrobial agents. It represents the most extensive survey of antimicrobial activity in Trigonella done to date.

References

. Mohana DC, Raveesha KA. Antibacterial activity of Caesalpinia coriaria (Jacq.) Willd. against plant pathogenic Xanthomonas pathovars: an ecofriendly approach. J Agric Technol. 2006; 2: 317-327.

. Dangi R: Diversity in genus Trigonella: Molecular and chemical characterization. PhD thesis. Pune University, Biotechnology Department; 2013.

. Acharya SN, JE Thomas, Basu SK. Fenugreek: an “old world” crop for the “new world”. Biodiversity. 2007; 7: 27-30.

. Bhatti MA, MTJ Khan, Ahmed B, Jamshaid M. Antimicrobial activity of Trigonella foenum-graecum seeds. Fitoterapia. 1996; 67: 372-374.

. De M, De AK, Banerjee AB. Antimicrobial screening of some Indian spices. Phytother Res. 1999; 13: 616-618.

. Wagh P, Rai M, Deshmukh SK, Durate MCT. Bio-activity of oils of Trigonella foenum-graecum and Pongamia pinnata. Afr J Biotechnol. 2007; 6: 1592-1596.

. Jain SC, Agrawal M, Sharma RA. The genus Trigonella – phytochemistry and biology. Anc Sci Life. 1996; 16: 108-117.

. Balodi B, Rao RR. The genus Trigonella L. (Fabaceae) in North-West Himalaya. J Econ Tax Bot. 1991; 15: 185-194.

. Petropoulos GA. Fenugreek. The genus Trigonella. Taylor and Francis, London and New York, 2002; pp: 225.

. Rajpal V. Standardization of Botanicals (testing and extraction methods of medicinal herbs) Eastern Publishers, New Delhi, India, 2002; pp: 39-46.

. Bylka W, Matlawska I, Pilewski NA. Natural flavonoids as antimicrobial agents. J Am Nutraceut Assoc. 2004; 7: 24-31.

. Pepeljnjak S, Kalodera Z, Zovko M. 2005. Antimicrobial activity of flavonoids from Pelargonium radula (Cav.) L 'Hérit. Acta. Pharm. 2005; 55: 431-435.

. Kim JH, Park ES, Shim JM, Kim MN, Moon WS, Chung KH, Yun JS. Antimicrobial activity of p-Hydroxyphenyl acrylate derivatives. J Agric Food Chem. 2004; 52: 7480-7483.

. Hussain M, Hanif M, Ali S, Butcher R, Mirza T, Vanderveer D, Aziz-ur-Rehman. In vitro biological studies and structural elucidation of fluoro-substituted phenyl acrylic acids. Drug Dev Ind Pharm. 2010; 36: 1079 -1087.

. Saleem M, Nazir M, Ali MS, Hussain H, Lee YS, Riaz N, Jabbar A. Antimicrobial natural products: an update on future antibiotic drug candidates. Nat Prod Rep. 2010; 27: 238-242.

. Osbourn A. Saponins and plant defence-a soap story. Trends Plant Sci., 1996; 1: 1-6.

. Morrissey JP, Osbourn AE. Fungal resistance to plant antibiotics as a mechanism of pathogenesis. Microbiol Mol Biol Rev. 1999; 63: 708-724.

. Morrissey JP, Wubben JP, Osbourn AE. 2000. Stagonospora avenae secretes multiple enzymes that hydrolyze oat leaf saponins. Mol Plant Microbe Interact. 2000; 13: 1041–1052.

. Greimel P, Spreitz J, Stütz AE, Wrodnigg TM. Iminosugars and relatives as antiviral and potential anti-infective agents. Curr Top Med Chem. 2003; 3: 513- 518

. Stütz AE. Iminosugars as Glycosidase Inhibitors: Nojirimycin and Beyond. New York, Wiley-VCH, 1999.

. Compain P, Marti OR. Design, synthesis and biological evaluation of iminosugar-based glycosyltransferase inhibitors. Curr Top Med Chem. 2003; 3: 541-560.

. Moriyama H, Tsukida T, Inoue Y, Yokota K, Yoshino K, Kondo H et al., Azasugar-based MMP/ADAM inhibitors as antipsoriatic agents. J Med Chem. 2004; 47: 1930-1938.

. Schramm VL, Tyler PC. Immino- sugar- based nucleosides. Curr Top Med Chem. 2003; 3: 525-540.

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Published

31-03-2016

How to Cite

1.
Rakhee Shyam Dangi, Dasharath Oulkar, Prashant Dhakephalkar, Sanjay Kumar Singh, Kaushik Banerjee, Dattatray Naik, Shubhada Tamhankar, Suryaprakasa Rao. Antimicrobial Activity of Some Trigonella Species. ijp [Internet]. 2016 Mar. 31 [cited 2024 Dec. 27];8(1):80-94. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/449

Issue

Vol. 8 No. 1 (2016): Jan-Mar

Section

Original Research Articles