Antimicrobial Activity of Some Trigonella Species

Authors

  • Rakhee Shyam Dangi Assistant Professor India Cell andMolecular Biology.
  • Dasharath Oulkar National research Centre for Grapes, Solapur road Pune Maharashtra India.
  • Prashant Dhakephalkar National research Centre for Grapes, Solapur road Pune Maharashtra India.
  • Sanjay Kumar Singh National research Centre for Grapes, Solapur road Pune Maharashtra India.
  • Kaushik Banerjee National research Centre for Grapes, Solapur road Pune Maharashtra India.
  • Dattatray Naik National research Centre for Grapes, Solapur road Pune Maharashtra India.
  • Shubhada Tamhankar National research Centre for Grapes, Solapur road Pune Maharashtra India.
  • Suryaprakasa Rao National research Centre for Grapes, Solapur road Pune Maharashtra India.

Keywords:

Antimicrobial activity;, Trigonella suavissima Lindl, T. spicata Sm, Saponins, Sapogenin

Abstract

The genus Trigonella includes many medicinal and aromatic plant species used in traditional as well as veterinary medicines for different diseases, alone or in combination with other remedies. The crude methanol extracts of 15 Trigonella species were assayed for antimicrobial activity against four medicinally important multidrug resistant clinical isolates, five plant pathogenic bacteria and five fungi. Three species showed a broad spectrum of antibacterial activity inhibiting all the test bacteria. Acidified ethanolic extract of the most promising species, T. suavissima was purified by column chromatography. Characterization of a partially purified ethyl acetate fraction by LC/ESI/MS showed the presence of 7’4’ dihydroxy flavone and (2E)-3-{5-[4-(Ethoxycarbonyl)phenyl]furan-2-yl}prop-2-enoate) Three species showed strong antifungal activity against Aspergillus niger and Fusarium solani. The saponin extract of T. spicata showed a selective activity against A. niger and the sapogenin extract against F. solani. A polyhydroxylated alkaloid was isolated from the sapogenin extract of T. spicata using chromatographic techniques with structural characterization done by LC/ESI/MS, 13C and 1H NMR. The study reveals that Trigonella species are potential sources of natural compounds that may act as antimicrobial agents. It represents the most extensive survey of antimicrobial activity in Trigonella done to date.

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Published

31-03-2016

How to Cite

1.
Rakhee Shyam Dangi, Dasharath Oulkar, Prashant Dhakephalkar, Sanjay Kumar Singh, Kaushik Banerjee, Dattatray Naik, Shubhada Tamhankar, Suryaprakasa Rao. Antimicrobial Activity of Some Trigonella Species. ijp [Internet]. 2016 Mar. 31 [cited 2024 Oct. 22];8(1):80-94. Available from: https://ijp.arjournals.org/index.php/ijp/article/view/449

Issue

Vol. 8 No. 1 (2016): Jan-Mar

Section

Original Research Articles