Study of Phytochemical screening,Total Phenolic and Flavonoid Content and Antioxidant Activity of Pulicaria glutinosa growing in Saudi Arabia


  • Jehan Yahya Al-humaidi Chemistry Department, College of Science, Princess Nuora Bint Saudi Arabia.


Antioxidant, DPPH, Flavonoids, Phenolic, P. glutinosa


Phytochemical screening of P. glutinosa extracts revealed the presence of alkaloids, terpenes, sponins, flavonids, anthraquinone and glycosides. Quantitative determination of total phenolics, total flavonoids, and various in vitro antioxidant activities (DPPH, ABTS metal chelation effect in the Fe2+-ferrozin test system) of the various extracts from the P. glutinosa was carried out. Phenol and flavonoid content of the extract were measured by folin ciocalteu and AlCl3 assays. The Phenol and flavonoid content of the extracts varied between 18.33 – 199.44 mg/g and 14.64 – 164.73 mg/g, respectively. The results showed that the butanol extract of P. glutinosa, which contain highest amount of phenol and flavonoid compounds, exhibited the greatest antioxidant activity in scavenging of DPPH free radical and ABTS radical cation assays. In other hands, results showed that aq. Methanol, butanol and water extracts studied are in metal-chelating assay lower active than EDTA and higher than ascorbic acid as a synthetic antioxidant.


. Alghaithy AA, El-Beshbishy HA, AbdelNaim AB, Nagy AA, Abdel-Sattar EM

Anti inflammatory effects of the

chloroform extract of Pulicaria guestii

ameliorated the neutrophil infiltration

and nitric oxide generation in rats.

Toxicol. Ind. Health. 2011;27: 899‒910.

. Al-Hazimi HMG, Al-Khathlan HZ).

Chemistry of Variuos Pulicaria spceies

(Asteraceae). Jour. Chem. Soc. Pak.

;14: 233-240.

. Al-Jaber HI, Abrouni K, Al-Qudah MA,

Abu Zarga MH New terpenes from

Salvia palaestina Benth. and Salvia

syriaca L. growing wild in Jordan. J

Asian Nat. Prod. Res. 2012;14: 618-25.

. Al-Qudah MA, Al-Ghoul AM, Trawenh

IN, Al-Jaber HI, Al Shboul TM, Abu

Zarga MH, Abu Orabi ST. Antioxidant

activity and chemical composition of

essential oils from Jordanian Ononis

Natrix L. and Ononis Sicula Guss.

JBAPN. 2014;4:52–61

. Al-Qudah MA, Al-Jaber HI, Abu Zarga

MH, Abu Orabi ST Flavonoid and

phenolic compounds from Salvia

palaestina L. growing wild in Jordan and

their antioxidant activities.

Phytochemistry, 2014;99:115-120.

. Ayoola GA, Coker HAB, Adesegun SA,

Adepoju-Bello AA, Obaweya K, Ezennia

EC, Atangbayila TO. Phytochemical

Screening and Antioxidant Activities of

Some Selected Medicinal Plants Used

for Malaria Therapy in Southwestern

Nigeria. Trop. J. Pharm. Res. 2008;


. Banso A, Adeyemo S. Phytochemical

screening and antimalarial assessment

of Abutilon mauritianum, Bacopa

monnifera and Datura stramonium.

Biokrmidtri. 2006;18:39-44

. Cai YZ, Sun M, Corke H. Antioxidant

activity of betalains from plants of the

Amaranthaceae. J. Agric. Food Chem.

;51: 2288–2294.

. Chlopicka J, Pasko P, Gorinstein S,

Jedryas A, Zagrodzki P. Total phenolic

and total flavonoid content, antioxidant

activity and sensory evaluation of

pseudocereal breads. LWT – Food Sci.

Tech. 2012;46: 548–555.

. Christine AW, Jeffrey BH, Jenny G,

Renee JG, Geoffrey CK, John E.

Variation in lipophilic and vacuolar

flavonoids among European Pulicaria

species. Phytochemistry. 2003;64: 275–

. Das B, Ravinder RM, Ramu R,

Ravindranath N, Harish H,

Ramakrishna KV, Koteswar RY,

Harakishore K, Murthy US. Clerodane

diterpenoids from Pulicaria wightiana.

Phytochemistry. 2005;66: 633–638.

. Dinis TCP, Madeira VMC, Almeida

MLM. Action of phenolic derivatives

(acetoaminophen, salycilate and 5-

aminosalycilate) as inhibitors of

membrane lipid peroxidation and as

peroxyl radical scavengers. Arch.

Biochem. Biophys. 1994;315: 161–169

. Ezoubeiri A, Gadhi CA, Fdil N,

Benharref A, Jana M, Vanhaelen M.

Isolation and antimicrobial activity of two

phenolic compounds from Pulicaria

odora L. J. Ethnopharmacol. 2005;99:


. Farooq, M. Neuro-protective activity of

Pulicaria glutinosa in oxidative stressinduced neurotoxicity in zebrafish

embryos. Int. J. Agric. Biol., 2015;17:


. Ghouila H, Beyaoui A, Jannet HB,

Hamdi B, Salah AH, Mighri Z. Isolation

and structure determination of

pulicazine, a new sesquiterpene lactone

from the Tunisian Pulicaria laciniata

( Kral.) Thell. Tetrahedron

Letters. 2009;50: 1563–1565.

. Joshi A, Bhobe M, Saatarkar A.

Phytochemical investigation of the roots

of Grewia microcos Linn. J. Chem.

Pharm. Res. 2013;5: 80–87.

. Khan M, Khan M, Adil SF, Tahir MN,

Tremel W, Alkhathlan Alkhathlan HZ,

Al-Warthan A, Siddiqui MRH. Green

synthesis of silver nanoparticles

mediated by Pulicaria glutinosa extract.

Int. J. Nanomedicine. 2013;8: 1507–

. Liu LL, Yang JL, Shi YP.

Phytochemicals and biological activities

of Pulicaria species. Chem.

Biodivers.2010;7: 327‒349.

. Marco JA, Sanz JF, Albiach R.

Caryophyllene derivatives from

Pulicaria dysenterica. Phytochemistry.

;31: 2409–2413.

. Mossa JS, Muhammad I, El-Feraly FS,

Huffor CD, Mcphail DR, McPhail AT.

Bisabolene and guaiane

sesquiterpenes from Pulicaria glutinosa.

Phytochemistry, 1992;31: 575-578.

. Muhammad I, El-Feraly FS, Mossa JS,

Ramadan AF. Terpenoids from

Pulicaria glutinosa. Phytochemistry.

;31: 4245–4248.

. Nickavar B, Mojab F. Antibacterial

activity of Pulicaria dysenterica extracts.

Fitoterapia. 2003;74: 390‒393.

. Singh P, Sharma MC, Joshi KC,

Bolhamann F. Diterpenes derived from

clerodanes from Pulicaria angustifolia.

Phytochemistry.1985;24: 190–192.

. Stavri M, Mathew KT, Gordon A,

Shnyder SD, Falconer RA, Gibbons S.

Guaianolide sesquiterpenes from

Pulicaria crispa (Forssk.) Oliv.

Phytochemistry.2008;69: 1915‒1918.

. Williams CA, Harborne JB, Greenham J.

Geographical variation in the surface

flavonoids of Pulicaria dysenterica.

Biochem. Syst. Ecol. 2000;28: 679‒

. Williams CA, Harborne JB, Greenham

JR, Grayer RJ, Kite GC, Eagles J.

Variations in lipophilic and vacuolar

flavonoids among European Pulicaria

species. Phytochemistry. 2003;64:


. Yamaguchi F, Ariga T, Yoshimura Y,

Nakazawa H. Antioxidative and antiglycation activity of garcinol from

Garcinia indica fruit rind. J. Agric. Food

Chem. 2000;48: 180–185.

. Zhao H, Fan W, Dong J, Lu J, Chen J,

Shan L. Evaluation of antioxidant

activities and total phenolic contents of

typical malting barley varieties. Food

Chemistry. 2008;107: 296.




How to Cite

Jehan Yahya Al-humaidi. Study of Phytochemical screening,Total Phenolic and Flavonoid Content and Antioxidant Activity of Pulicaria glutinosa growing in Saudi Arabia. ijp [Internet]. 2016 Mar. 31 [cited 2024 Jul. 15];8(1):62-8. Available from:



Original Research Articles